Which Biomarkers Are Effective for Identifying Th2-Driven Inflammation in Asthma?

Diamant, Zuzana; Tufvesson, Ellen; Bjermer, Leif

doi:10.1007/s11882-013-0376-6

Cited by 11 publications

(9 citation statements)

References 109 publications

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“…Such a signaling cascade shares high similarities with the T H 2 cytokine-mediated allergic responses in human asthma [7], [48], [49]. In our studies, levels of IL-4, IL-5, and IL-13 all strikingly increased in the OVA-stimulated mice, indicating that our model reliably activated the T H 2 type of immune responses.…”

Section: Discussionsupporting

confidence: 68%

Protective Effects of the Polyphenol Sesamin on Allergen-Induced TH2 Responses and Airway Inflammation in Mice

et al. 2014

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Allergic asthma is a lifelong airway condition that affects people of all ages. In recent decades, asthma prevalence continues to increase globally, with an estimated number of 250,000 annual deaths attributed to the disease. Although inhaled corticosteroids and b-adrenergic receptor agonists are the primary therapeutic avenues that effectively reduce asthma symptoms, profound side effects may occur in patients with long-term treatments. Therefore, development of new therapeutic strategies is needed as alternative or supplement to current asthma treatments. Sesamin is a natural polyphenolic compound with strong anti-oxidative effects. Several studies have reported that sesamin is effective in preventing hypertension, thrombotic tendency, and neuroinflammation. However, it is still unknown whether sesamin can reduce asthma-induced allergic inflammation and airway hyperresponsiveness (AHR). Our study has revealed that sesamin exhibited significant anti-inflammatory effects in ovalbumin (OVA)-induced murine asthma model. We found that treatments with sesamin after OVA sensitization and challenge significantly decreased expression levels of interleukin-4 (IL-4), IL-5, IL-13, and serum IgE. The numbers of total inflammatory cells and eosinophils in BALF were also reduced in the sesamin-treated animals. Histological results demonstrated that sesamin attenuated OVA-induced eosinophil infiltration, airway goblet cell hyperplasia, mucus occlusion, and MUC5AC expression in the lung tissue. Mice administered with sesamin showed limited increases in AHR compared with mice receiving vehicle after OVA challenge. OVA increased phosphorylation levels of IkB-a and nuclear expression levels of NF-kB, both of which were reversed by sesamin treatments. These data indicate that sesamin is effective in treating allergic asthma responses induced by OVA in mice.

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Section: Discussionsupporting

confidence: 68%

Protective Effects of the Polyphenol Sesamin on Allergen-Induced TH2 Responses and Airway Inflammation in Mice

et al. 2014

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“…Asthmatic patients typically have elevated levels of Th2 cytokines in their BALF and bronchial mucosa [ 7 ]. It is well known that Th2-activated cells orchestrate pulmonary immune responses and mediate the inflammation of lung tissues, as well as airway hyperresponsiveness [ 8 – 10 ]. Th1-activated cells also inhibit the inflammation of asthmatic airways [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Loki zupa (Luooukezupa) decoction reduced airway inflammation in an OVA-induced asthma mouse model

Wei

Abduwaki

et al. 2016

Chin Med

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BackgroundLoki zupa (Luooukezupa) decoction, consisting of the roots of Hyssopuscuspidatus Boriss (Shenxiangcao) and Irishalophila Pall root (Yuanweigen), is commonly used in Uygur medicine to treat asthma. However, the mode of action of this material has yet to be elucidated. This study aims to investigate the effects of Loki zupa decoction on the airway inflammation of an ovalbumin (OVA)-induced asthma mouse model.MethodsMice were divided into normal control (NC), asthma (A), high, medium and low doses of Loki zupa decoction (L 14.0, L 7.0, L 3.5), water extract (LW), n-butanol extract (LN), ethyl acetate extract (LE) and dexamethasone (DEX) groups. Antiasthmatic model was induced by OVA sensitization and challenged using BALB/c mice. Airway hyperresponsiveness (AHR) toward methacholine (Mch) was assessed using Buxco equipment. Lung inflammation was measured by hematoxylin and eosin staining and bronchoalveolar lavage fluid (BALF) cell count and classification. Inflammatory cytokines in BALF and serum were analyzed by Bio-Plex assay, and mRNA levels were investigated by qPCR analysis. The roots of H. Boriss (250 g) and I. Pall (250 g) were decocted, concentrated and diluted to 14.0, 7.0 and 3.5 g crude herb/kg body weight. The LW, LN and LE of the Loki zupa decoction were prepared and diluted to a dose equivalent to 7 g of crude herb/kg body weight.ResultsLoki zupa decoction and its extracts significantly attenuated the AHR towards Mch (all P < 0.05). Treatment with Loki zupa decoction and its extracts relieved the infiltration of inflammatory cells in and around the airways, and reduced the total white blood cell (all P < 0.05), neutrophil (all P < 0.05), monocyte (all P < 0.05) and eosinophil (all P < 0.05) counts in the BALF. The BALF samples collected from the mice treated with the Loki zupa decoction and its extracts had lower levels of IL-1β (all P < 0.05), TNF-α (all P < 0.05), IL-2 (all P < 0.05), IL-4 (P = 0.047) and IL-5 (all P < 0.05). The serum samples of these mice also had lower IL-1β (all P < 0.05), TNF-α (all P < 0.05), IL-4 (all P < 0.05) and IL-5 (all P < 0.05) levels and higher levels of IFN-γ (P < 0.001) compared with the OVA-induced asthma mouse model. qPCR analysis revealed that Loki zupa decoction and its extracts inhibited mRNA expression of IL-4 (all P < 0.05), IL-5 (all P < 0.05) and IL-13 (all P < 0.05) and promoted mRNA expression of IFN-γ (all P < 0.05) in asthmatic mice.ConclusionLoki zupa decoction reduced AHR, attenuated airway inflammation, promoted Th1 and suppressed Th2 cell functions in an OVA-induced asthma mouse model.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-016-0094-9) contains supplementary material, which is available to authorized users.

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“…However, uncontrolled T‐cell responses can cause tissue and organ damage. For example, overshoot of Th1 and Th17 cells can be associated with autoimmune and inflammatory diseases, while Th2 cell over‐activation can lead to allergic disorders . Activated immune responses are kept under control by a regulatory network, with regulatory T (Treg) cells at the forefront, which express suppressory molecules, capable of controlling activated effector T cells .…”

Section: Introductionmentioning

confidence: 99%

“…For example, overshoot of Th1 and Th17 cells can be associated with autoimmune and inflammatory diseases, 20,21 while Th2 cell over-activation can lead to allergic disorders. 22 Activated immune responses are kept under control by a regulatory network, with regulatory T (Treg) cells at the forefront, which express suppressory molecules, capable of controlling activated effector T cells. 23 Interleukin-22 (IL-22) produced by a distinct Th22 cell subset, 18 or by Th17 cells in combination with IL-17, 24 has been reported in inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

Changes in immunological profile as a function of urbanization and lifestyle

et al. 2014

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Summary Differences in lifestyle and break with natural environment appear to be associated with changes in the immune system resulting in various adverse health effects. Although genetics can have a major impact on the immune system and disease susceptibility, the contribution of environmental factors is thought to be substantial. Here, we investigated the immunological profile of healthy volunteers living in a rural and an urban area of a developing African country (Senegal), and in a European country (the Netherlands). Using flow cytometry, we investigated T helper type 1 (Th1), Th2, Th17, Th22 and regulatory T cells, as well as CD4+ T‐cell and B‐cell activation markers, and subsets of memory T and B cells in the peripheral blood. Rural Senegalese had significantly higher frequencies of Th1, Th2 and Th22 cells, memory CD4+ T and B cells, as well as activated CD4+ T and B cells compared with urban Senegalese and urban Dutch people. Within the Senegalese population, rural paritcipants displayed significantly higher frequencies of Th2 and Th22 cells, as well as higher pro‐inflammatory and T‐cell activation and memory profiles compared with the urban population. The greater magnitude of immune activation and the enlarged memory pool, together with Th2 polarization, seen in rural participants from Africa, followed by urban Africans and Europeans suggest that environmental changes may define immunological footprints, which could have consequences for disease patterns in general and vaccine responses in particular.

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Which Biomarkers Are Effective for Identifying Th2-Driven Inflammation in Asthma?

Cited by 11 publications

References 109 publications

Protective Effects of the Polyphenol Sesamin on Allergen-Induced TH2 Responses and Airway Inflammation in Mice

Protective Effects of the Polyphenol Sesamin on Allergen-Induced TH2 Responses and Airway Inflammation in Mice

Loki zupa (Luooukezupa) decoction reduced airway inflammation in an OVA-induced asthma mouse model

Changes in immunological profile as a function of urbanization and lifestyle

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