WHIRLY1 recruits the histone deacetylase HDA15 repressing leaf senescence and flowering in <i>Arabidopsis</i>

Huang, Dongmei; Lan, Wei; Ma, Weibo; Huang, Rulin; Lin, Wenfang; Li, Meng-Si; Chen, Chia-Yang; Wu, Keqiang; Miao, Ying

doi:10.1111/jipb.13272

Cited by 20 publications

(18 citation statements)

References 77 publications

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“…Our results indicate that WHIRLY1 may act as a scaffold protein in these complexes. WHIRLY1 physically interacts with histone deacetylase HDA15, as shown by yeast two-hybrid, bimolecular fluorescence complementation and co-immunoprecipitation, and both proteins co-localize to the region near the transcription start site of some nutrient-recycling-related genes [ 55 ]. This led to the assumption that WHIRLY1 indeed acts as a repressor via recruiting HDA15, which causes the deacetylation of associated histones.…”

Section: Discussionmentioning

confidence: 99%

WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications

Manh

Ost

Peiter

et al. 2023

IJMS

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WHIRLY1, a small plant-specific ssDNA-binding protein, dually located in chloroplasts and the nucleus, is discussed to act as a retrograde signal transmitting a stress signal from the chloroplast to the nucleus and triggering there a stress-related gene expression. In this work, we investigated the function of WHIRLY1 in the drought stress response of barley, employing two overexpression lines (oeW1-2 and oeW1-15). The overexpression of WHIRLY1 delayed the drought-stress-related onset of senescence in primary leaves. Two abscisic acid (ABA)-dependent marker genes of drought stress, HvNCED1 and HvS40, whose expression in the wild type was induced during drought treatment, were not induced in overexpression lines. In addition, a drought-related increase in ABA concentration in the leaves was suppressed in WHIRLY1 overexpression lines. To analyze the impact of the gain-of-function of WHIRLY1 on the drought-related reprogramming of nuclear gene expression, RNAseq was performed comparing the wild type and an overexpression line. Cluster analyses revealed a set of genes highly up-regulated in response to drought in the wild type but not in the WHIRLY1 overexpression lines. Among these genes were many stress- and abscisic acid (ABA)-related ones. Another cluster comprised genes up-regulated in the oeW1 lines compared to the wild type. These were related to primary metabolism, chloroplast function and growth. Our results indicate that WHIRLY1 acts as a hub, balancing trade-off between stress-related and developmental pathways. To test whether the gain-of-function of WHIRLY1 affects the epigenetic control of stress-related gene expression, we analyzed drought-related histone modifications in different regions of the promoter and at the transcriptional start sites of HvNCED1 and HvS40. Interestingly, the level of euchromatic marks (H3K4me3 and H3K9ac) was clearly decreased in both genes in a WHIRLY1 overexpression line. Our results indicate that WHIRLY1, which is discussed to act as a retrograde signal, affects the ABA-related reprogramming of nuclear gene expression during drought via differential histone modifications.

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Section: Discussionmentioning

confidence: 99%

WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications

Manh

Ost

Peiter

et al. 2023

IJMS

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“…The oeUPL3-GFP , oeUBP12-GFP , and oeGFP plants from the fifth to eighth rosette leaves of 6-wk-old plants were harvested and ground in liquid nitrogen. Total proteins were extracted from 1 g of materials and performed CoIP according to the description by Huang et al (2022) .…”

Section: Methodsmentioning

confidence: 99%

Ubiquitome profiling reveals a regulatory pattern of UPL3 with UBP12 on metabolic-leaf senescence

Lan

Zheng

et al. 2022

Life Sci. Alliance

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The HECT-type UPL3 ligase plays critical roles in plant development and stress protection, but understanding of its regulation remains limited. Here, the multi-omics analyses of ubiquitinated proteins in upl3 mutants were performed. A landscape of UPL3-dependent ubiquitinated proteins is constructed: Preferential ubiquitination of proteins related to carbon fixation represented the largest set of proteins with increased ubiquitination in the upl3 plant, including most of carbohydrate metabolic enzymes, BRM, and variant histone, whereas a small set of proteins with reduced ubiquitination caused by the upl3 mutation were linked to cysteine/methionine synthesis, as well as hexokinase 1 (HXK1) and phosphoenolpyruvate carboxylase 2 (PPC2). Notably, ubiquitin hydrolase 12 (UBP12), BRM, HXK1, and PPC2 were identified as the UPL3-interacting partners in vivo and in vitro. Characterization of brm, upl3, ppc2, gin2, and ubp12 mutant plants and proteomic and transcriptomic analysis suggested that UPL3 fine-tunes carbohydrate metabolism, mediating cellular senescence by interacting with UBP12, BRM, HXK1, and PPC2. Our results highlight a regulatory pattern of UPL3 with UBP12 as a hub of regulator on proteolysis-independent regulation and proteolysis-dependent degradation.

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“…A study showed that C2H2-type zinc finger MdZAT10 can accelerate the aging of apple leaves ( Yang et al., 2021 ). In Arabidopsis, knocking out HDA15 can accelerate leaf aging and promote plant flowering ( Huang et al., 2022 ). In safflower, CtCP1 promotes the hydrolysis of the CtbHLH41 protein, accelerating the transcriptional activity of related genes and leaf senescence ( Hong et al., 2022 ).…”

Section: Leaf Senescence and Horticultural Plant Yieldmentioning

confidence: 99%

Research progress on the relationship between leaf senescence and quality, yield and stress resistance in horticultural plants

Zhao

Wang

et al. 2022

Front. Plant Sci.

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Leaf senescence, the final stage of leaf development, is one of the adaptive mechanisms formed by plants over a long period of evolution. Leaf senescence is accompanied by various changes in cell structure, physiological metabolism, and gene expressions. This process is controlled by a variety of internal and external factors. Meanwhile, the genes and plant hormones involved in leaf aging affect the quality, yield and stress resistance in horticultural plants. Leaf senescence mediated by plant hormones affected plant quality at both pre-harvest and post-harvest stages. Exogenous plant growth regulators or plant hormone inhibitors has been applied to delay leaf senescence. Modification of related gene expression by over-expression or antisense inhibition could delay or accelerate leaf senescence, and thus influence quality. Environmental factors such as light, temperature and water status also trigger or delay leaf senescence. Delaying leaf senescence could increase chloroplast lifespan and photosynthesis and thus improve source strength, leading to enhanced yield. Accelerating leaf senescence promotes nutrient redistribution from old leaves into young leaves, and may raise yield under certain circumstances. Many genes and transcriptional factors involved in leaf senescence are associated with responses to abiotic and biotic stresses. WRKY transcriptional factors play a vital role in this process and they could interact with JA signalling. This review summarized how genes, plant hormones and environmental factors affect the quality, yield. Besides, the regulation of leaf senescence holds great promise to improving the resistance to plant biotic and abiotic stresses.

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WHIRLY1 recruits the histone deacetylase HDA15 repressing leaf senescence and flowering in Arabidopsis

Cited by 20 publications

References 77 publications

WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications

WHIRLY1 Acts Upstream of ABA-Related Reprogramming of Drought-Induced Gene Expression in Barley and Affects Stress-Related Histone Modifications

Ubiquitome profiling reveals a regulatory pattern of UPL3 with UBP12 on metabolic-leaf senescence

Research progress on the relationship between leaf senescence and quality, yield and stress resistance in horticultural plants

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