Whisker stimulation increases expression of nerve growth factor- and interleukin-1β-immunoreactivity in the rat somatosensory cortex

Hallett, Heather; Churchill, Lynn; Taishi, Ping; De, Alok; Krueger, James M.

doi:10.1016/j.brainres.2010.03.048

Cited by 40 publications

(34 citation statements)

References 67 publications

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“…There are several studies that demonstrated the expression of other IEGs or related proteins, such as c-Fos, in the S1BF. Manual whisker stimulation over the course of 8 days increased the number of growth factors within layers II–V in somatosensory cortex [32]. Also using immunohistochemistry, Filipkowski et al (2000) showed two novel approaches to induce whisker stimulation-initiated changes in gene expression of c-Fos in the S1BF: 1) manual whisker stimulation of one side of the rat’s myastacial pad for 20 minutes and 2) placing the rat in a novel wire cage in a dark sound-proof room for 20 minutes.…”

Section: Discussionmentioning

confidence: 99%

The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation

Khodadad

Adelson

Lifshitz

et al. 2015

Behavioural Brain Research

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Immediate early genes have previously demonstrated a rapid increase in gene expression after various behavioral paradigms. The main focus of this article is to identify a molecular marker of circuit activation after manual whisker stimulation or exploration of a novel environment. To this end, we investigated the dynamics of ARC transcription in adult male rats during whisker somatosensation throughout the whisker barrel circuit. At various time points, tissue was biopsied from the ventral posterior medial nucleus (VPM) of the thalamus, primary somatosensory barrel field (S1BF) cortex and hippocampus for quantification using real-time PCR and western blot. Our results show that there were no significant differences in ARC gene or protein expression in the VPM after both types of stimulation. However, manual whisker stimulation resulted in increased ARC gene expression at 15, 30, 60 and 300 minutes in the S1BF, and 15 minutes in the hippocampus (p<0.05). Also, exploration of a novel environment resulted in increased ARC mRNA expression at 15 and 30 minutes in the S1BF and at 15 minutes in the hippocampus (p<0.05). The type of stimulation (manual versus exploration of a novel environment) influenced the magnitude of ARC gene expression in the S1BF (p<0.05). These data are the first to demonstrate that ARC is a specific, quantifiable and input dependent molecular marker of circuit activation which can serve to quantify the impact of brain injury and subsequent rehabilitation on whisker sensation.

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Section: Discussionmentioning

confidence: 99%

The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation

Khodadad

Adelson

Lifshitz

et al. 2015

Behavioural Brain Research

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“…Waking neuronal activity drives the production of cytokines, establishing a link between local sleep intensity and prior waking activity. Expression of TNF-a and IL-1b is elevated in somatosensory cortex in response to whisker stimulation [56,57]. Both neurons and astrocytes are potential sources of cytokines in neocortex [26].…”

mentioning

confidence: 99%

Hypothalamic versus neocortical control of sleep

Szymusiak¹

2010

Current Opinion in Pulmonary Medicine

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The evidence for use-dependent modulation of neocortical activity during sleep is compelling and provides novel insights into sleep function. However, local changes in neocortex are generally expressed on a background of global sleep. It remains to be determined if events initiated in the cortex have global sleep-promoting effects and how neocortical and hypothalamic mechanisms of sleep control interact.

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“…IL1R is activated by IL1β, a known sleep regulatory molecule [1,5,6,7,8]. We show that this signaling mechanism must be intact in order for hypersomnolence to occur subsequent to METH-induced wakefulness.…”

Section: Discussionmentioning

confidence: 88%

Interleukin 1 receptor contributes to methamphetamine- and sleep deprivation-induced hypersomnolence

Schmidt

Wisor

2012

Neuroscience Letters

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Methamphetamine-induced wakefulness is dependent on monoamine transporter blockade. Subsequent to methamphetamine-induced wakefulness, the amount of time spent asleep and the depth of sleep are increased relative to baseline sleep. The mechanisms that drive methamphetamine-induced hypersomnolence are not fully understood. We recently observed that methamphetamine exposure elevates the expression of the sleep-promoting cytokine, interleukin-1 β in CD11b-positive monocytes within the brain. Here, we sought to determine whether activation of the interleukin 1 receptor (IL1R) drives the increase in the depth and amount of sleep that occurs subsequent to methamphetamine-induced wakefulness. IL1R-deficient mice and wild type control mice were subjected to systemic methamphetamine (1 and 2mg/kg) and saline treatments. The wake-promoting effect of methamphetamine was modestly potentiated by IL1R-deficiency. Additionally, the increase in time spent in NREMS subsequent to methamphetamine-induced wakefulness in wild type mice was abolished in IL1R-deficient mice. The increase in time spent asleep after 3 h of behaviorally enforced wakefulness was also abolished in IL1R-deficient mice. Increases in EEG slow wave activity triggered by methamphetamine and sleep deprivation were of equal magnitude in IL1R-deficient and wild type mice. These data demonstrate that IL1R activation contributes to hypersomnolence that occurs after sleep loss, whether that sleep loss is triggered pharmacologically by methamphetamine or through behavioral sleep deprivation.

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Whisker stimulation increases expression of nerve growth factor- and interleukin-1β-immunoreactivity in the rat somatosensory cortex

Cited by 40 publications

References 67 publications

The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation

The time course of activity-regulated cytoskeletal (ARC) gene and protein expression in the whisker-barrel circuit using two paradigms of whisker stimulation

Hypothalamic versus neocortical control of sleep

Interleukin 1 receptor contributes to methamphetamine- and sleep deprivation-induced hypersomnolence

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