White Cell and Thrombocytie Disorders

Arachidonic acid in the reticuloendothelial network

“…However, network analysis will yield new insights into the investigation of arachidonic acid activity in both dietary and acute experiments. Such approaches are already being implemented in the analysis of phagocyte activation [35,36]. This analysis has identified ultrastructural changes associated with Ca2+ mobilization and immunological activation.…”

“…Network approaches may therefore be especially useful in the analysis of the multifactorial changes associated with pathophysiological events such as trauma, cancer and cardiovascular disease, conditions in which arachidonic acid may play a mediatory role [1,36]. T o carry out network analysis, calculations of arachidonic acid flux similar to those defined for PtdIns(4,5)P2 by Stephens et al [ 141 must be associated with parameters of cell activation and cell-cell inactivation.…”

Medical and surgical applications of FELs

“…[12] allows the assessment of such effects on a large number of cells in very short time by analysing intracellular fluorescent markers, tracing and measuring simultaneously 6 key metabolites. A self classifying software, developed by VALET [13] identifies typical damage patterns and correlates them to biochemical pathways as a function of the external stimuli, e.g. the incident laser radiation.…”