White clover fractions as protein source for monogastrics: dry matter digestibility and protein digestibility‐corrected amino acid scores

Stødkilde, Lene; Damborg, Vinni Kragbæk; Jørgensen, Henry; Lærke, Helle Nygaard; Jensen, Søren Krogh

doi:10.1002/jsfa.8744

Cited by 45 publications

(43 citation statements)

References 39 publications

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“…Compared to soybean meal commonly used in feed (Lagos and Stein, 2017), both ryegrass and red clover protein had well-balanced amino acid composition with favorable content of essential amino acids (Figure 8). Previous studies with proteins extracted from white clover, red clover and grass and precipitated by traditional methods obtained similar results and also documented good digestibility of the produced protein concentrates (Stødkilde et al, 2018(Stødkilde et al, , 2019. The effect of lignosulfonate on digestibility therefore remains to be investigated.…”

Section: Juice Clearing and Purity Of Protein Concentratementioning

confidence: 76%

“…The soluble protein is separated from the juice and turned into a protein concentrate. Feeding trials with protein concentrate obtained from white clover, red clover, perennial grass and alfalfa have documented a high digestibility and a wellbalanced amino acid composition, which makes it a promising protein source for monogastrics (Stødkilde et al, 2018(Stødkilde et al, , 2019.…”

Section: Introductionmentioning

confidence: 99%

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Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Cour

Schjøerring

Jørgensen

2019

Front. Sustain. Food Syst.

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Biorefining of green biomass to produce proteins for feed and food provides an important challenge in relation to development of a future sustainable and climateneutral agriculture. In order to make it a viable value chain, all parts of the process need to be optimized. This includes careful selection of the source of biomass, the procedure used for protein extraction as well as the separation and recovery of proteins from the press juice. We here focus on recovery of proteins from juice made from screw press fractionation of four green biomasses, viz. ryegrass, red clover, ryegrasswhite clover mixture and spinach. Separating out protein from the juice is a key step and several different methods have routinely been applied. We demonstrate that lignosulfonates aid in the precipitation of proteins in the juice. The optimal loading of lignosulfonate was 0.6-0.7 g per g crude protein in the juice. For ryegrass and red clover juice, this loading resulted in 10-25% increase in precipitated protein compared to the classical precipitation by heat treatment or acidification. For spinach, up to 60% increase in protein recovery was obtained by lignosulfonate addition. We conclude that lignosulfonate-assisted precipitation holds potential for improving the protein yield in biorefining of green biomass.

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Section: Juice Clearing and Purity Of Protein Concentratementioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Cour

Schjøerring

Jørgensen

2019

Front. Sustain. Food Syst.

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“…Previously, protein concentrates with slightly higher TP contents were produced from grass clover (35% DM) or red clover (40% DM) in laboratory setups . Moreover, protein concentrates produced by acid precipitation with sulfuric acid (pH = 4.0) at lab scale from the whole white clover plant presented CP content of 45% DM and TP contents of 39% DM, while higher CP content of 53% DM and higher TP content of 44% DM were achieved when the protein concentrate was produced only from white clover leaves . The protein concentrate presented a low pH (4.2) and had some lactic acid (6.1% DM), glucose (1.4% DM), and a considerable amount of inorganics (10.6% DM).…”

Section: Resultsmentioning

confidence: 99%

“…The chemical composition of the protein concentrate produced organically from grass clover biomass in the demon- clover plant presented CP content of 45% DM and TP contents of 39% DM, while higher CP content of 53% DM and higher TP content of 44% DM were achieved when the protein concentrate was produced only from white clover leaves. 34 The protein concentrate presented a low pH (4.2) and had some lactic acid (6.1% DM), glucose (1.4% DM), and a considerable amount of inorganics (10.6% DM). The concentration of each amino acid was slightly lower compared with the protein concentrates produced from clover grass and red clover in laboratory setups, 14 as shown in Table 4.…”

Section: Composition and Amino Acid Profile Of The Organic Protein Comentioning

confidence: 97%

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Santamaría-Fernández

Ambye‐Jensen

Damborg

et al. 2018

Biofuels Bioprod Bioref

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There is an increasing interest in green biorefineries for the utilization of green biomass to provide a variety of valuable products. Proteins can be extracted from green biomasses such as alfalfa, clover, or grass and separated into protein‐rich concentrates suitable for feeding to monogastric animals. Press cake suitable for feeding ruminant animals is also produced. Full implementation of such a green biorefinery requires knowledge and experience of production at demonstration scale. Moreover, product evaluation in animal‐feeding trials requires a large amount of product. There has been collaboration in Denmark between several partners from industry and academia to assess the practicality and scalability of a green biorefining plant producing protein concentrate and press cake silage from organic grass clover at the scale of ca. 10 tons per hour fresh biomass input. Lactic acid fermentation of the pressed green juice was successfully performed to precipitate proteins and stabilize the juice. Despite minor difficulties, the demonstration‐scale biorefining plant was operative for four days, processed 444 tons of grass clover, and resulted in the overall production of 1 ton of dried protein concentrate, 7 tons of protein paste, and 223 tons of press cake silage. The protein concentrate was rich in essential amino acids and was tested in feeding trials with poultry; the press cake silage, rich in processed fibers and fiber‐associated proteins, was tested in feeding trials with dairy cows. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…The CP content in the final protein extract and protein digestibility have been shown to be affected by DM and CP content of the unprocessed plant (Pirie, 1978). Accordingly, Stødkilde et al (2018), Stødkilde et al (2019) showed in a rat model that protein extraction improved digestibility compared to the unprocessed plant. The AA composition in the protein extract was well balanced from a nutritional point of view, and the content of methionine was superior to the content in soybean and soybean meal (National Research Council, 2012;SEGES Svineproduktion, 2018) and to earlier findings on white clover, red clover and lucerne (Damborg et al, 2018;Stødkilde et al, 2018Stødkilde et al, , 2019.…”

Section: Composition Of Protein Extract Produced From Grass-clovermentioning

confidence: 99%

Biorefined grass‐clover protein composition and effect on organic broiler performance and meat fatty acid profile

Stødkilde

Ambye‐Jensen

Jensen

2020

Animal Physiology Nutrition

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The organic poultry sector is rapidly growing in certain parts of the European Union, and a 14% yearly increase was registered in the organic poultry head numbers between 2005 and 2015 (European Commission, 2016). Optimising organic feed formulations with respect to the animals amino acid (AA) requirement, without feeding a surplus of protein, is a challenge, as supplementation with free AA is not allowed in the organic production system (Burley, Anderson, Patterson, & Tillman, 2016). Moreover, the permission to include 5% non-organic feed in organic feed formulations, used mainly for balancing the AA composition of the feed, will be phased out in the European Union. This will cause an increased need for alternative protein sources with the desired AA composition for optimal

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White clover fractions as protein source for monogastrics: dry matter digestibility and protein digestibility‐corrected amino acid scores

Abstract: A high digestibility of white clover protein was found irrespective of the physical fractionation. Together with a well-balanced amino acid composition, this makes white clover a promising protein source for monogastrics. © 2017 Society of Chemical Industry.

Cited by 45 publications

References 39 publications

Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Enhancing Protein Recovery in Green Biorefineries by Lignosulfonate-Assisted Precipitation

Demonstration‐scale protein recovery by lactic acid fermentation from grass clover – a single case of the production of protein concentrate and press cake silage for animal feeding trials

Biorefined grass‐clover protein composition and effect on organic broiler performance and meat fatty acid profile

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