White light diffraction phase microscopy with slightly off-axis blind two-step phase-shifting

Ma, Lihong; Zhang, Jiaheng; Xin, 杨鑫 Yang; Li, Yong; Jin, Weimin

doi:10.1016/j.optcom.2019.124563

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…It's obvious that the carrier frequency can be easily adjusted by tilting M2 to satisfy the sampling conditions for the interferogram [25,26]. In this case, the real, conjugate and DC orders of the interferogram can be separated completely in the Fourier spectrum.…”

Section: Principlementioning

confidence: 99%

“…2006, Popescu proposed the diffraction phase microscopy (DPM) [21] by combining a 4f optical system with a diffraction grating at input plane and a hole-array at the Fourier plane, which is a very famous and advantage PDI. Then, several improved DPMs [22][23][24][25][26] are also proposed. However, due to the only utilization of 0th and 1st diffraction orders, the grating causes a large power loss.…”

Section: Introductionmentioning

confidence: 99%

“…The proposed module employs off-the-shelf optical elements to build the amplitudedivision interferometric module, resulting in a compact, simple, easy and low-cost implementation. Compared to current common-path off-axis iQPMs [22][23][24][25][26][27][28][29][30], the proposed method can freely regulate each channel to achieve the ideal frequency and/or visibility of the interferogram, but does not require special optical skills and/ or complex alignment. Due to the single-shot off-axis acquisition, the proposed method can retain both temporally and spatially sensitive measured capabilities, which is mainly limited by the measurement rate and resolution of the employed camera [31,32].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quasi-common-path off-axis interferometric quantitative phase microscopy based on amplitude-division

et al. 2023

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A quasi-common-path off-axis interferometric quantitative phase microscopy (iQPM) is proposed using amplitude-division in this paper. Utilizing a quasi-common-path interferometric module, our method divides the object beam into two copies using a beam-splitter and two mirrors after the output of the microscope, while spatially filters one of the copies to act as the reference beam, and thus yields off-axis interference at the camera plane. The compact module is built using simple optical elements without the requirements of special optical skills and/or complex alignment. In contrast to the previous common-path off-axis iQPMs, the proposed method can flexibly adjust each channel to improve the visibility and/or frequency of the interferogram. Several experimental results are presented to demonstrate the validity and stability of the proposed iQPM.

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Section: Principlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quasi-common-path off-axis interferometric quantitative phase microscopy based on amplitude-division

et al. 2023

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“…As a QPI technique, white light diffraction phase microscopy (WDPM) enables 3D phase profile measurements of RBCs with single shot measurement and low speckle noise [8,[20][21][22][23]. WDPM is a slightly off-axis interferometry which has the properties of high temporal phase stability and spatial phase sensitivity with single shut image [24].…”

Section: Introductionmentioning

confidence: 99%

White light diffraction phase microscopy for imaging of red blood cells for different storage times

Kocahan,

Çelebioğlu,

Uyanık

2024

Phys. Scr.

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In this study, the effects of different storage times on the surface morphology of red blood cells (RBCs) were investigated using white light diffraction phase microscopy (WDPM). Blood samples collected from 10 volunteer and stored for 56 days, were imaged on WDPM at every 7 days without any sample preparation. To obtain the phase profiles of RCBs, first the sample and then the reference interferograms were obtained from the experimental setup. Then, surface profiles were calculated from these interferogram images using Fourier transform (FT). With the experiment performed every 7 days, 10 RBC phase information were obtained from each sample and surface profiles were created. From these profiles, 7 parameters related with RBC morphology (average cell thickness – ACT; mean corpuscular volume – MCV; projected surface area – PSA; total surface area – SA; diameter – D; mean corpuscular haemoglobin – MCH; surface area to volume ratio - SAV) have been calculated. Therefore, changes in the morphology of RBCs during storage were evaluated quantitatively. Additionally, phase calibration target was used to confirm the accuracy of our experimental setup system. From the interferogram images, the depth of the phase target (GRP 9 and element 3) was obtained as 309 nm, in accordance with the produced depth. By this way, the reliability of the WDPM setup is demonstrated. This study suggests that the morphology of RBCs can be quantitatively obtained in a reliable manner at higher resolution with WDPM without sample preparation.

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“…Optical interferometer [1][2][3][4][5][6][7][8][9] is a promising tool for quantitative phase imaging [4][5][6][7][8][9][10], and has been applied wildly in the measurement of micro structures, biological specimens and surface profiles. According to the coincidence of the object beam path and the reference beam path, the interferometer can be categorized into two types: separated-path interferometer [1][2][3] and common-path interferometer [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Single shot point-diffraction interferometer by a plate beamsplitter

Shan,

Yin,

Zhong

et al. 2024

Phys. Scr.

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A single shot point-diffraction interferometer is proposed in this paper, in which one plate beamsplitter is assembled with one mirror and one pinhole array to duplicate an object beam and generate one object beam and one reference beam. One mirror is placed perpendicularly at the rear of the optical 4f system, while one thin plate beamsplitter is placed ahead of the mirror with an inclined angle. With the help of the half-transmission and half-reflection of the plate beamsplitter, one object beam is duplicated into to copies. Owning to the different angles of the plate beamsplitter and the mirror with respect to the optical axis, after Fourier transformed by the second Lens of the 4f system and reflected by the cube beamsplitter, the two copies are separated in the spectral domain. Then, one object beam and one reference beam are generated by the pinhole array placed at the spectral plane. The object and reference beams are Fourier transformed by the third Lens and interferes to generate an off-axis interferogram at the camera. By adjusting the inclined angle of the plate beamsplitter, the carrier frequency of the interferogram can be modulated easily. Therefore, this interferometer has a simple optical setup, easy optical implementation and outstanding measurement ability with high precision, measurement efficiency and stability. Several experimental results will be provided to demonstrate the effectiveness of the proposed system.

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White light diffraction phase microscopy with slightly off-axis blind two-step phase-shifting

Cited by 10 publications

References 24 publications

Quasi-common-path off-axis interferometric quantitative phase microscopy based on amplitude-division

Quasi-common-path off-axis interferometric quantitative phase microscopy based on amplitude-division

White light diffraction phase microscopy for imaging of red blood cells for different storage times

Single shot point-diffraction interferometer by a plate beamsplitter

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