White light reflectance spectroscopy biosensing system for fast quantitative prostate specific antigen determination in forensic samples

Koukouvinos, Georgios; Metheniti, Aristea; Karachaliou, Chrysoula-Evangelia; Goustouridis, D.; Livaniou, Evangelia; Μισιακός, Κωνσταντίνος; Raptis, Ioannis; Kondili, A.; Miniati, P.; Petrou, Panagiota; Kakabakos, Sotirios

doi:10.1016/j.talanta.2017.07.074

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…In Figure 7a, the CRP calibration curves obtained using chips modified with 2% The stability of the anti-CRP antibodies immobilized onto chips modified with APTES following different protocols was also tested through the performance of repeated cycles of immunoreactions and surface regeneration, that is, removal of immune complexes formed during the assay. For surface regeneration, a 0.1 M glycine-HCl buffer, pH 2.5, was used, which has been determined as optimal for this particular antibody in previous publications (Koukouvinos et al, 2018;Koukouvinos, Metheniti, et al, 2017;Koukouvinos, Τsialla, et al, 2017). The results obtained from chips modified with 2% ( F I G U R E 7 (a) Typical CRP calibration curves obtained from chips modified with 2% (v/v) APTES in water (black squares), 5% (v/v) APTES in ethanol (red squares) and 5% (v/v) APTES in ethanol and glutaraldehyde (blue squares).…”

Section: Development Of Wlrs Sensor For the Immunochemical Determinmentioning

confidence: 99%

Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

et al. 2020

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Section: Development Of Wlrs Sensor For the Immunochemical Determinmentioning

confidence: 99%

Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

et al. 2020

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“…This significant reduction of the assay time was attributed to the shrinkage of the fluidic cell dimensions (form 100 μL to 20 μL) that allowed for quicker diffusion of the reagents towards the immobilized probe molecules. This fast PSA assay was implemented to determine PSA concentration in forensic cases samples, including swabs and fabrics collected by alleged crime scenes (mainly alleged rape cases) [ 56 ]. The results were compared with a semi-quantitative immunochromatographic strip currently used by forensic labs for PSA detection, and the presence or absence of semen was verified by microscopic detection of spermatozoa and male DNA identification through Y chromosome detection.…”

Section: System Development and Applicationsmentioning

confidence: 99%

Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform

et al. 2017

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The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low molecular weight analytes (e.g., pesticides), is described. At the same time, the passage from single to multi-analyte determinations, and from a laboratory prototype set-up to a compact device appropriate for on-site determination, is outlined. The improvements made on both the sensor and the optical set-up, and the concomitant advances in the analytical characteristics and the robustness of the assays performed with the different layouts, are also presented. Finally, the future perspectives of the system, aiming for the creation of a standalone instrument to be used by non-experts, will be discussed.

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“…Until now, several analytical techniques have been developed for determination of PSA and Myo individually. These include chromatography [8,9], mass spectroscopy [10,11], fluorescence spectroscopy [12,13], nuclear magnetic resonance spectroscopy [14,15], white light reflectance spectroscopy [16,17], capillary electrophoresis [18], chemiluminescence [19], enzyme-linked immunosorbent assays (ELISA) [20,21], electrochemiluminescence [22,23], radioimmunoassay [24,25], time-resolved immunofluorometric assay [26], surface plasmon fluorescence immunoassay [27], bioluminescent immunoassay [28], electrochemical [29], surface-enhanced Raman scattering [24,30] and microcantilever method [31]. Of these methods, biosensors especially electrochemical types can offer some advantages in contrast to the commonly used sensing tools, including simultaneous analysis of biomarkers, capability of miniaturization, lower cost and simplicity of analysis [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer

Karami¹,

Bagheri

Johari-Ahar

et al. 2019

Talanta

120

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A new dual-modality immunosensor based on molecularly imprinted polymer (MIP) and a nanostructured biosensing layer has fabricated for the simultaneous detection of two important markers including prostatespecific antigen (PSA) and myoglobin (Myo) in human serum and urine samples. In the first step, 3,3′-dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP) was self-assembled on a gold screen printed electrode (SPE). Then, the target proteins were attached covalently to the DSP-SPE. The imprinted cocktail polymer ((MIP (PSA, Myo)-SPE)) was synthesized at the SPE surface using acrylamide as monomer, N,N′-methylenebisacrylamide as a crosslinker, and PSA and Myo as the templates, respectively. The MIP-SPE was specific for the impedimetric sensing of PSA and Myo. After that, a nanocomposite (NCP) was synthesized based on the decorated magnetite nanoparticles with multi-walled carbon nanotube, graphene oxide and specific antibody for PSA (Ab). Then, NCP incubated with (MIP(PSA, Myo)-SPE. The modified electrodes and synthesized nanoparticles were characterized using electrochemical impedance spectroscopy, dynamic light scattering, surface plasmon resonance and scanning electron microscopy. The limits of detections were found to be 5.4 pg mL −1 and 0.83 ng mL −1 with the linear dynamic ranges of 0.01-100 and 1-20000 ng mL −1 for PSA and Myo, respectively. The ability of proposed biosensor to detect PSA and Myo simultaneously with high sensitivity and specificity offers a powerful opportunity for the new generation of biosensors. This dual-analyte specific receptors-based device is highly desired for the integration with lab-on-chip kits to measure a wide panel of biomarkers present at ultralow levels during early stages of diseases progress.

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White light reflectance spectroscopy biosensing system for fast quantitative prostate specific antigen determination in forensic samples

Cited by 11 publications

References 25 publications

Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

Functionalization of silicon dioxide and silicon nitride surfaces with aminosilanes for optical biosensing applications

Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform

Dual-modality impedimetric immunosensor for early detection of prostate-specific antigen and myoglobin markers based on antibody-molecularly imprinted polymer

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