White Lupin: A Model System for Understanding Plant Adaptation to Low Phosphorus Availability

Uhde‐Stone, Claudia

doi:10.1007/978-3-319-55729-8_13

Cited by 11 publications

(13 citation statements)

References 173 publications

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“…In fact, the -P plants in this study presented a 42% higher shoot to root ratio than C plants ( Figure 3A ). As expected, white lupin plants produced huge amounts of cluster roots to cope with P deficiency ( Uhde-Stone, 2017 ) ( Figure 3E ). Cluster root formation is very complex and probably modulated by signaling hormones ( e.g.…”

Section: Discussionsupporting

confidence: 74%

“…auxin, nitric oxide), sucrose and miRNAs ( e.g. miR399) ( Liu and Vance, 2010 ; Wang et al., 2015 ; Uhde-Stone, 2017 ; Zhou et al., 2019 ). Additionally, cluster roots can be also formed in plants with sufficient P nutrition, although those cluster roots are not fully functional ( Meng et al., 2013 ; Wang et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…White lupin ( Lupinus albus L., Fabaceae family) is a non-mycorrhizal plant, which is well known to exude huge amounts of carboxylates, especially under P starvation ( Mimmo et al., 2011 ; Marschner, 2012 ; Valentinuzzi et al., 2015 ). Therefore, this species serves often as a model plant in studies of root induced mobilization of sparingly soluble soil P sources ( Uhde-Stone, 2017 ). Under P deficiency some plant species of the Proteaceae family develop special root types, the so-called proteoid roots (or cluster roots), which are short lateral rootlets with a bottlebrush-like appearance ( Dinkelaker et al., 1989 ).…”

Section: Introductionmentioning

confidence: 99%

“…Under P deficiency some plant species of the Proteaceae family develop special root types, the so-called proteoid roots (or cluster roots), which are short lateral rootlets with a bottlebrush-like appearance ( Dinkelaker et al., 1989 ). These cluster roots are extremely efficient in enhancing soil P availability due to the increase of the roots surface area by more than 100-fold and to the exudation of huge concentrations of protons, carboxylates and phosphatases, particularly in comparison with non-proteoid roots ( Skene, 2000 ; Uhde-Stone, 2017 ). Furthermore, cluster roots also release large concentrations of flavonoids ( e.g.…”

Section: Introductionmentioning

confidence: 99%

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Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots

et al. 2020

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The reliable quantification of root exudation and nutrient uptake is a very challenging task, especially when considering single root segments. Most methods used necessitate root handling e.g. root dissecting/cutting. However, there is a knowledge gap on how much these techniques affect root physiology. Thus, this study aimed at assessing the effect of different root handling techniques on the phosphate (P i ) uptake and carboxylate exudation of white lupin roots. White lupin plants were grown hydroponically in a full and P i -deficient nutrient solution for 60 days. Phosphate uptake and carboxylate exudation of cluster and non-cluster roots were measured using custom made cells 1, 4, and 8 h after the onset of light. Three different experimental set-ups were used: i) without cutting the root apparatus from the shoots, nor dissecting the root into smaller root sections — named intact plant (IP); ii) separating the roots from the shoots, without dissecting the root into smaller sections — named intact root (IR); iii) separating the roots form the shoots and dissecting the roots in different sections—named dissected roots (DR). The sampling at 8 h led to the most significant alterations of the root P i uptake induced by the sampling method. Generally, roots were mainly affected by the DR sampling method, indicating that results of studies in which roots are cut/dissected should be interpreted carefully. Additionally, the study revealed that the root tip showed a very high P i uptake rate, suggesting that the tip could act as a P i sensor. Citrate, malate and lactate could be detected in juvenile, mature and senescent cluster root exudation. We observed a significant effect of the handling method on carboxylate exudation only at sampling hours 1 and 8, although no clear and distinctive trend could be observed. Results here presented reveal that the root handling as well as the sampling time point can greatly influence root physiology and therefore should not be neglected when interpreting rhizosphere dynamics.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots

et al. 2020

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“…Legumes have evolved conserved acquisition and internal transport strategies for P i detected in P i -deficient soils in order to maintain nodule P i -homeostasis and enable efficient SNF ( Figure 2 ). These include decreased plant growth rates, modification of carbon metabolism, increased secretion of organic anions and phosphatases, changes in root architecture, expansion of root surface areas, and enhanced expression of P i transporters ( Sulieman and Tran, 2015 ; Considine et al, 2017 ; Kleinert et al, 2017 ; Uhde-Stone, 2017 ). Because of the diminishing reserves of inexpensive P i fertilizers, plant acclimation to P i deficiency has become a topic of considerable interest to plant researchers.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics and Transcriptomics in Legumes Under Phosphate Deficiency in Relation to Nitrogen Fixation by Root Nodules

et al. 2018

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Phosphate (Pi) deficiency is a critical environmental constraint that affects the growth and development of several legume crops that are usually cultivated in semi-arid regions and marginal areas. Pi deficiency is known to be a significant limitation for symbiotic nitrogen (N2) fixation (SNF), and variability in SNF is strongly interlinked with the concentrations of Pi in the nodules. To deal with Pi deficiency, plants trigger various adaptive responses, including the induction and secretion of acid phosphatases, maintenance of Pi homeostasis in nodules and other organs, and improvement of oxygen (O2) consumption per unit of nodule mass. These molecular and physiological responses can be observed in terms of changes in growth, photosynthesis, and respiration. In this mini review, we provide a brief introduction to the problem of Pi deficiency in legume crops. We then summarize the current understanding of how Pi deficiency is regulated in legumes by changes in the transcriptomes and metabolomes found in different plant organs. Finally, we will provide perspectives on future directions for research in this field.

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The genome evolution and low-phosphorus adaptation in white lupin

Zhang

Yuan

et al. 2020

Nat Commun

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White lupin (Lupinus albus) is a legume crop that develops cluster roots and has high phosphorus (P)-use efficiency (PUE) in low-P soils. Here, we assemble the genome of white lupin and find that it has evolved from a whole-genome triplication (WGT) event. We then decipher its diploid ancestral genome and reconstruct the three sub-genomes. Based on the results, we further reveal the sub-genome dominance and the genic expression of the different sub-genomes varying in relation to their transposable element (TE) density. The PUE genes in white lupin have been expanded through WGT as well as tandem and dispersed duplications. Furthermore, we characterize four main pathways for high PUE, which include carbon fixation, cluster root formation, soil-P remobilization, and cellular-P reuse. Among these, auxin modulation may be important for cluster root formation through involvement of potential genes LaABCG36s and LaABCG37s. These findings provide insights into the genome evolution and low-P adaptation of white lupin.

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White Lupin: A Model System for Understanding Plant Adaptation to Low Phosphorus Availability

Cited by 11 publications

References 173 publications

Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots

Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots

Metabolomics and Transcriptomics in Legumes Under Phosphate Deficiency in Relation to Nitrogen Fixation by Root Nodules

The genome evolution and low-phosphorus adaptation in white lupin

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