White lupin (<i>Lupinus albus</i>) variation for adaptation to severe drought stress

Annicchiarico, Paolo; Romani, Massimo; Pecetti, L.

doi:10.1111/pbr.12642

Cited by 23 publications

(21 citation statements)

References 26 publications

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“…Affected regulatory pathways include, among others, the flowering time control (Nelson et al, 2010). The rapid flowering of domesticated germplasm may favor drought escape and adaptation for spring sowing in higher latitudes (Annicchiarico et al, 2010(Annicchiarico et al, , 2018Berger et al, 2017). However, the observed extension of the vegetation period has raised the demand for germplasm with intermediate phenology and cross-environment adaptation.…”

Section: Pal Allele Carrying Intermediate Phenology and Light Vernalimentioning

confidence: 99%

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin (Lupinus angustifolius L.)

et al. 2020

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Narrow-leafed lupin (Lupinus angustifolius L.) is a moderate-yielding legume crop known for its high grain protein content and contribution to soil improvement. It is cultivated under photoperiods ranging from 9 to 17 h, as a spring-sown (in colder locations) or as an autumn-sown crop (in warmer regions). Wild populations require a prolonged cold period, called vernalization, to induce flowering. The key achievement of L. angustifolius domestication was the discovery of two natural mutations (named Ku and Jul) conferring vernalization independence. These mutations are overlapping deletion variants in the promoter of LanFTc1, a homolog of the Arabidopsis thaliana FLOWERING LOCUS T (FT) gene. The third deletion, named here as Pal, was recently found in primitive germplasm. In this study, we genotyped L. angustifolius germplasm that differs in domestication status and geographical origin for LanFTc1 alleles, which we then phenotyped to establish flowering time and vernalization responsiveness. The Ku and Jul lines were vernalization-independent and early flowering, wild (ku) lines were vernalization-dependent and late flowering, whereas the Pal line conferred intermediate phenotype. Three lines representing Ku, Pal, and ku alleles were subjected to gene expression surveys under 8-and 16-h photoperiods. FT homologs (LanFTa1, LanFTa2, LanFTc1, and LanFTc2) and some genes selected by recent expression quantitative trait loci mapping were analyzed. Expression profiles of LanFTc1 and LanAGL8 (AGAMOUSlike 8) matched observed differences in flowering time between genotypes, highlighted by high induction after vernalization in the ku line. Moreover, these genes revealed altered circadian clock control in Pal line under short days. LanFD (FD) and LanCRLK1 (CALCIUM/CALMODULIN-REGULATED RECEPTOR-LIKE KINASE 1) were negatively responsive to vernalization in Ku and Pal lines but positively responsive or variable in ku, whereas LanUGT85A2 (UDP-GLUCOSYL TRANSFERASE 85A2) was significantly suppressed by vernalization in all lines. Such a pattern suggests the opposite regulation of these gene pairs in the vernalization pathway. LanCRLK1 and LanUGT85A2 are homologs of A. thaliana genes involved in the FLOWERING LOCUS C (FLC) vernalization

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Section: Pal Allele Carrying Intermediate Phenology and Light Vernalimentioning

confidence: 99%

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin (Lupinus angustifolius L.)

et al. 2020

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“…Introduction of preferred alleles on one-by-one basis can be inefficient, due to epistatic interactions between major components of flowering regulatory network [ 90 ]. Phenotyping studies revealed that white lupin germplasm collection has variability of many agronomic traits, including, besides time to flowering, winter survival, pod fertility, pod wall proportion, number of leaves on the main stem, several yield related traits and anthracnose resistance [ 11 , 12 , 13 , 29 , 91 , 92 , 93 , 94 ]. Despite relatively small genotype sample size (83 landraces and eight varieties), genomic prediction for grain yield, winter plant survival and onset of flowering provided prospective output, manifested by model-based predictive ability values as high as 0.84–0.86 [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…For many years, wide use of lupins for human consumption was hampered by their bitter taste caused by a high alkaloid content, but this issue has been fixed in modern cultivars [ 9 ]. Considering the agronomic characteristics of white lupin, there is still high potential for further yield increases and drought tolerance improvement by exploitation of existing germplasm resources [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Control of Early Flowering in White Lupin (Lupinus albus L.)

Rychel-Bielska

Surma

Bielski

et al. 2021

IJMS

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White lupin (Lupinus albus L.) is a pulse annual plant cultivated from the tropics to temperate regions for its high-protein grain as well as a cover crop or green manure. Wild populations are typically late flowering and have high vernalization requirements. Nevertheless, some early flowering and thermoneutral accessions were found in the Mediterranean basin. Recently, quantitative trait loci (QTLs) explaining flowering time variance were identified in bi-parental population mapping, however, phenotypic and genotypic diversity in the world collection has not been addressed yet. In this study, a diverse set of white lupin accessions (n = 160) was phenotyped for time to flowering in a controlled environment and genotyped with PCR-based markers (n = 50) tagging major QTLs and selected homologs of photoperiod and vernalization pathway genes. This survey highlighted quantitative control of flowering time in white lupin, providing statistically significant associations for all major QTLs and numerous regulatory genes, including white lupin homologs of CONSTANS, FLOWERING LOCUS T, FY, MOTHER OF FT AND TFL1, PHYTOCHROME INTERACTING FACTOR 4, SKI-INTERACTING PROTEIN 1, and VERNALIZATION INDEPENDENCE 3. This revealed the complexity of flowering control in white lupin, dispersed among numerous loci localized on several chromosomes, provided economic justification for future genome-wide association studies or genomic selection rather than relying on simple marker-assisted selection.

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“…Modern breeding programs mainly focus on yield stabilization, resistance to abiotic stresses, biochemical structure associated with seed quality, resistance to diseases (mainly to anthracnose) and late maturing [ 30 , 77 , 78 ].…”

Section: Breeding Of Lupinsmentioning

confidence: 99%

“…The main research work has been focused on identification of genetic material that can be utilized in breeding programs. In Italy 21 landraces, one commercial variety and two breeding lines were screened under severe drought stress and favourable conditions through a phenotyping platform that revealed substantial variation in white lupin available genetic material [ 134 ]. Two yellow lupin ( L. luteus ) cultivars were tested under drought conditions in order to examine how manipulation of polyamine biosynthesis by using inhibitors of their biosynthesis can influence lupin drought tolerance but the results are not straightforward [ 135 ].…”

Section: Breeding Of Lupinsmentioning

confidence: 99%

The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches

Abraham

Ganopoulos

Madesis

et al. 2019

IJMS

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Livestock production in the European Union EU is highly dependent on imported soybean, exposing the livestock farming system to risks related to the global trade of soybean. Lupin species could be a realistic sustainable alternative source of protein for animal feeding. Lupinus is a very diverse genus with many species. However, only four of them—namely, L. albus, L. angustifolius, L. luteus and L. mutabilis—are cultivated. Their use in livestock farming systems has many advantages in relation to economic and environmental impact. Generally, lupin grains are characterized by high protein content, while their oil content is relatively low but of high quality. On the other hand, the presence of quinolizidine alkaloids and their specific carbohydrate composition are the main antinutritional factors that prevent their use in animal feeding. This research is mainly related to L. albus and to L. angustifolius, and to a lesser extent, to L. lauteus and L. mutabilis. The breeding efforts are mostly focused on yield stabilization, resistance to biotic and abiotic stresses, biochemical structure associated with seed quality and late maturing. Progress is made in improving lupin with respect to the seed quality, as well as the tolerance to biotic and abiotic stress. It has to be noted that modern cultivars, mostly of L. albus and L. angustifolius, contain low levels of alkaloids. However, for future breeding efforts, the implementation of marker-assisted selection and the available genomic tools is of great importance.

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White lupin (Lupinus albus) variation for adaptation to severe drought stress

Cited by 23 publications

References 26 publications

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin (Lupinus angustifolius L.)

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin (Lupinus angustifolius L.)

Quantitative Control of Early Flowering in White Lupin (Lupinus albus L.)

The Use of Lupin as a Source of Protein in Animal Feeding: Genomic Tools and Breeding Approaches

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