White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus

Li, Xiaoliang; Zhang, Chulong; Fang, Weihuan; Lin, Fu‐Cheng

doi:10.1631/jzus.b0720009

Cited by 45 publications

(17 citation statements)

References 12 publications

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“…The soft-shelled turtle Pelodiscus sinensis is a commercially important aquatic reptile species because of its high nutritional and medicinal value in Asian countries including China, Japan, and Korea (Li et al, 2008;Gong et al, 2011). In China, it is considered to be a rich delicious food with medical benefits.…”

Section: Introductionmentioning

confidence: 99%

De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

Wang

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:The soft-shelled turtle Pelodiscus sinensis is a high-profile turtle species because of its nutritional and medicinal value in Asian countries. However, little is known about the genes that are involved in formation of their nutritional quality traits, especially the molecular mechanisms responsible for unsaturated fatty acid and collagen biosynthesis. In the present study, the transcriptomes from six tissues from Pelodiscus sinensis were sequenced using an Illumina paired-end sequencing platform. We obtained more than 47 million sequencing reads and 73 954 unigenes with an average size of 754 bp by de-novo assembly. In total, 55.19% of the unigenes (40 814) had significant similarity with proteins in the National Center of Biotechnology Information (NCBI) non-redundant protein database and Swiss-Prot database (E-value <10 −5 ). Of these annotated unigenes, 9 156 and 11 947 unigenes were assigned to 52 gene ontology categories (GO) and 25 clusters of orthologous groups (COG), respectively. In total, 26 496 (35.83%) unigenes were assigned to 242 pathways using the Kyoto Encyclopedia of Genes and Genomes pathway database (KEGG). In addition, we found a number of highly expressed genes involved in the regulation of P. sinensis unsaturated fatty acid biosynthesis and collagen formation, including desaturases, growth factors, transcription factors, and extracellular matrix components. Our data represent the most comprehensive sequence resource available for the Chinese soft-shelled turtle and could provide a basis for new research on this turtle as well as the molecular genetics and functional genomics of other terrapins. To our knowledge, we report for the first time, the large-scale RNA sequencing (RNA-Seq) of terrapin animals and would enrich the knowledge of turtles for future research.

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Section: Introductionmentioning

confidence: 99%

De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

Wang

et al. 2013

J. Zhejiang Univ. Sci. B

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“…The Chinese soft-shelled turtle (Trionyx sinensis) belongs to reptile, living in fresh water and known as a health-promoting food in Asian countries including China, Japan and Korea [16]. Along with rapid development of large-scale farmed culture in southeastern Chinese provinces Zhejiang, Jiangsu and Fujian, farms raising the Chinese soft-shelled turtles have suffered serious economic losses due to infectious diseases caused by microbial pathogens such as Aeromonas hydrophila [17].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and functional analysis of MyD88 in Chinese soft-shelled turtle Trionyx sinensis

Zhu

Chen

et al. 2011

Fish & Shellfish Immunology

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“…Fusarium semitectum Berk. & Ravenel was isolated from Texas tortoises Gopherus berlandieri (Rose et al 2001), and Paecilomyces lilacinus Samson was isolated from Chinese soft-shelled turtles (Trionyx sinens) (Li et al 2008). In 1996 Aphanomyces infection in juvenile soft-shelled turtles imported from Singapore.…”

Section: Introductionmentioning

confidence: 99%

Aphanomyces sinensis sp. nov., isolated from juvenile soft-shelled turtle, Pelodiscus sinensis, in Japan

et al. 2011

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A species of Aphanomyces was isolated from juvenile soft-shelled turtles, Pelodiscus sinensis, cultured in Japan. Typically, an infected turtle showed small whitish maculae on the carapace. Many hyphae were observed in the epidermis. The hyphae were isolated using glucoseyeast (GY) agar plates. The morphological characteristics were very similar to those of Aphanomyces laevis, but a clear nuclear spot was observed in the center of the oospore in the strains isolated from the soft-shelled turtles. The optimal growth temperature for the isolates was 25-30°C and the optimum pH was 6-9. Experimental infection tests with isolates produced small whitish maculae on the carapace, and soft-shelled turtles artificially infected with the zoospores showed high mortality, especially in the highdose group. Phylogenetic analysis based on the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) indicated that the isolates from the soft-shelled turtles were unidentified species of Aphanomyces. As a result, the strain was described as a new species, Aphanomyces sinensis.

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White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus

Cited by 45 publications

References 12 publications

De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

De-novo characterization of the soft-shelled turtle Pelodiscus sinensis transcriptome using Illumina RNA-Seq technology

Molecular characterization and functional analysis of MyD88 in Chinese soft-shelled turtle Trionyx sinensis

Aphanomyces sinensis sp. nov., isolated from juvenile soft-shelled turtle, Pelodiscus sinensis, in Japan

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