White-spot syndrome virus diagnostics in frozen shrimp stocks imported to Mexico

Reyes‐López, Miguel Ángel; Salazar-Marroquín, E. L.; Oliva-Hernández, Amanda Alejandra; Salinas-López, N.; Narváez-Zapata, José Alberto

doi:10.1080/11358120902907030

Cited by 6 publications

(10 citation statements)

References 17 publications

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“…6). It has been earlier reported that WSSV DNA was detected by PCR in frozen cold stored shrimp products (Nunan et al 1998;Durand et al 2000;McColl et al 2004;Reville et al 2005;Hasson et al 2006;Reyes-Ló pez et al 2009). The detection of WSSV DNA in cold stored shrimps by the single step PCR with the primer pair of 1s5 & 1a16 showed that this primer set is suitable for the detection of degraded WSSV DNA, as reported by East et al (2005).…”

Section: Discussionmentioning

confidence: 91%

Effect of processing treatments on the white spot syndrome virus DNA in farmed shrimps (Penaeus monodon)

Reddy

Jeyasekaran

Shakila

2011

Letters in Applied Microbiology

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Aims: To investigate the effect of processing treatments on the destruction of white spot syndrome virus (WSSV) DNA in WSSV‐infected farmed shrimps (Penaeus monodon). Methods and Results: The presence of WSSV was tested by single step and nested polymerase chain reaction (PCR). The primers 1s5 & 1a16 and IK1 & IK2 were used for the single step PCR and primers IK1 & IK2–IK3 & IK4 were used for the nested PCR. Various processing treatments such as icing, freezing, cooking, cooking followed by slow freezing, cooking followed by quick freezing, canning, and cold storage were employed to destroy the WSSV DNA. Of the processing treatments given, cooking followed by quick freezing was efficient in destroying WSSV DNA in WSSV‐infected shrimp products. Canning, and cooking followed by slow freezing process had some destructive effect on the WSSV DNA, as WSSV DNA in such processed shrimp products was detected only by nested PCR. Icing, slow freezing, quick freezing, and cooking processes had no effect on the destruction of WSSV DNA. A gradual increase in the destruction of WSSV DNA was observed as the cold storage period increased. Conclusion: The results indicated that cooking followed by quick freezing process destroy the WSSV DNA. Significance and Impact of the Study: WSSV can be destroyed by cooking followed by quick freezing and this combined process can reduce the disease transmission risks from commodity shrimps to native shrimps.

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Section: Discussionmentioning

confidence: 91%

Effect of processing treatments on the white spot syndrome virus DNA in farmed shrimps (Penaeus monodon)

Reddy

Jeyasekaran

Shakila

2011

Letters in Applied Microbiology

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“…The detection level of WSSV by nested PCR was found to be very high with 73% incidence in the farmed frozen shrimp products when compared to single step PCR ( Table 2). The primers (IK1-IK2 & IK3-IK4) used in nested PCR yielded an amplicon of 310 bp (Figure 3) Many authors have also suggested the use of nested PCR to increase the accuracy of viral diagnostics particularly WSSV from shrimps [6,11,24]. A normal harvest would not give any indication of infection status of shrimp products with respective important shrimp viral pathogens [15] and in such cases usually the nested PCR give positive results for the target viral pathogens.…”

Section: Detection Of Wssv In Farmed Frozen Shrimp Productsmentioning

confidence: 99%

“…But, [10] Reville et al have detected WSSV in only 4.7% of shrimp products in supermarkets of Massachusetts, USA. In Mexico [6] Lopez et al reported the incidence of WSSV in frozen shrimp products as 32%. The variation in prevalence of WSSV in shrimp products observed by different workers might be due to the differences in the pattern of outbreaks of WSSV in shrimp farms at different point of time of sampling.…”

Section: Detection Of Wssv In Farmed Frozen Shrimp Productsmentioning

confidence: 99%

“…The spread of exotic viruses in farmed shrimps can be attributed to several reasons like transportation of infected stocks, by birds acting as vectors and carrying the diseases and by importation & processing of frozen food products [2,3]. Several authors studied the presence of white spot syndrome virus (WSSV) in shrimps by using PCR [4][5][6]. It has been reported that the transmission of exotic viral pathogens to farmed and native shrimp stocks may be possible via frozen prawn products prepared and packaged for human consumption [7].…”

Section: Introductionmentioning

confidence: 99%

“…Viral disease diagnostics on imported fresh and frozen stocks have been hardly implemented and have only included stock samples produced in Latin America or Asia [3,10,11,6]. It is listed as a non-exotic disease in EC directive 2006/88 [12,13] examined the populations of Australian crustaceans for evidence of White Spot Syndrome virus (WSSV) and found neither mortalities, clinical signs of disease nor evidence of WSSV.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Infectious Salmon Anaemia Virus (ISAV) Ringtest: Validation of the ISAV Diagnositic Process using Virus-spiked Fish Tissues and ISAV TaqMan® Real-time RT-PCR

Kibenge¹,

Kibenge²,

Masaoud³

et al. 2010

J Aquac Res Development

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The incidence of white spot syndrome virus (WSSV) in the Indian farmed frozen shrimp products viz., whole, headless (HL) and peeled and undeveined (PUD) by polymerase chain reaction (PCR) was studied. The viability of WSSV in farmed frozen shrimps was investigated by conducting bio-inoculation studies. The products were screened for WSSV by both the single step (for the primer sets 1s5 & 1a16 and IK1 & IK2) and nested PCR (for the primers IK1 & IK2-IK3 & IK4). Single step PCR detected WSSV in 18% of samples, while the nested PCR detected WSSV in 73% of samples. The frozen material, which gave positive results for WSSV by PCR, was further confirmed for the viability of WSSV by the bio-inoculation studies. Mortality (100%) was observed within the 45 h of intra-muscular post injection (P.I) into the healthy wild live WSSV-free shrimps (Penaeus monodon). These results show that the WSSV survived the freezing process and the cold storage and could cause infection in native shrimp farms if such products are reprocessed in the importing countries.

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Diagnostic Molecular Investigation of White Spot Syndrome Virus Finds No Infection in Wild White Shrimp and Brown Shrimp along the Texas Gulf Coast

et al. 2021

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White spot syndrome virus (WSSV) is a virulent disease that disrupts shrimp farm operations throughout the world. While the United States has had only limited outbreaks of WSSV within the past several decades, it is important to ensure that this disease does not infect wild penaeid shrimp populations. In Texas, there is a potential for WSSV to spread to wild penaeid populations in the Gulf of Mexico via infected imported nonnative bait shrimp, imported broodstock, or wild crustacean hosts. Due to these potential threats, the Texas Parks and Wildlife Coastal Fisheries Division monitored WSSV in wild brown shrimp Farfantepenaeus aztecus and white shrimp Litopenaeus setiferus from seven major bay systems along the Texas coast during 2019. While no positive samples were detected from the collected shrimp, a power analysis illustrated a potential for low‐level WSSV prevalence within Texas shrimp populations that would not be detectable by this monitoring survey. Overall, WSSV does not appear to be a major threat in the Texas region of the Gulf of Mexico, but continual observation and monitoring of wild penaeid shrimp is necessary to protect this resource from future WSSV outbreaks.

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White-spot syndrome virus diagnostics in frozen shrimp stocks imported to Mexico

Cited by 6 publications

References 17 publications

Effect of processing treatments on the white spot syndrome virus DNA in farmed shrimps (Penaeus monodon)

Effect of processing treatments on the white spot syndrome virus DNA in farmed shrimps (Penaeus monodon)

Infectious Salmon Anaemia Virus (ISAV) Ringtest: Validation of the ISAV Diagnositic Process using Virus-spiked Fish Tissues and ISAV TaqMan® Real-time RT-PCR

Diagnostic Molecular Investigation of White Spot Syndrome Virus Finds No Infection in Wild White Shrimp and Brown Shrimp along the Texas Gulf Coast

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