White spot syndrome virus VP37 interacts with VP28 and VP26

Liu, Qinghui; Ma, Cuiyan; Chen, Wenbo; Zhang, Xiuli; Liang, Yan; Dong, Shuanglin; Huang, Jie

doi:10.3354/dao02050

Cited by 27 publications

(27 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such homologous genes have been found in WSSV VP28, VP26 and VP24 [11], and further studies demonstrated that VP24 interacts with VP28 and is involved in virus infection [15]. We have reported that VP37 interacts with VP28 and VP26 [8]. Until now, there is little information concerning the role of VP292.…”

Section: Discussionmentioning

confidence: 94%

“…The other procedures were the same as above. Recombinant VP28 was constructed as previously reported [8].…”

Section: Wssv Envelop and Nucleocapsid Protein Preparedmentioning

confidence: 99%

“…The desired recombinant protein was purified using a column of Ni-nitriloacetic acid resins under denatured conditions and renatured by successive 12 h incubations with 6, 4, 2 and 0 M guanidine-HCl in buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 25 mM dithiothreitol, 0.1 % Tween-20, 10 % glycerol, pH 7.5). Purified VP292 was labeled with digoxigenin (DIG) [8].…”

Section: Wssv Envelop and Nucleocapsid Protein Preparedmentioning

confidence: 99%

“…Envelope proteins are important for virus infection and assembly. In previous studies, it has been shown that the function of envelope proteins was involved in virus infection and some WSSV structural proteins interact with other structure proteins [1,2,8,15].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

VP292 of White spot syndrome virus Interacts with VP26

Liu

Huang

2012

Indian J. Virol.

View full text Add to dashboard Cite

Interactions between virus structural proteins are suggested to be crucial for virus assembly. Many steps in the process of white spot syndrome virus (WSSV) assembly and maturation remain unclear. In this paper, we discovered a new interaction of WSSV VP292. Temporaltranscription analysis showed that VP292 is expressed in the late stage of WSSV infection. Western blot and matrixassisted laser desorption ionization MS assays showed that VP292 interacts with VP26, a major envelope protein. Farwestern blot provided further evidence for interaction between VP292 and VP26. These results collectively demonstrated that VP292 anchors to the envelope through interaction with VP26.

show abstract

Section: Discussionmentioning

confidence: 94%

“…The other procedures were the same as above. Recombinant VP28 was constructed as previously reported [8].…”

Section: Wssv Envelop and Nucleocapsid Protein Preparedmentioning

confidence: 99%

Section: Wssv Envelop and Nucleocapsid Protein Preparedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

VP292 of White spot syndrome virus Interacts with VP26

Liu

Huang

2012

Indian J. Virol.

View full text Add to dashboard Cite

show abstract

“…Since VP24 is a major viral protein and also interacts with other structural proteins, it has been speculated that groups of these proteins form a basic frame work for the virus upon which the other proteins build up [18]. Liu et al [27] attempted to study the morphology of WSSV by interaction study. The overlay assay and the subsequent His-pull down assay confirmed the interaction of VP37 with VP26 and VP28.…”

Section: Protein Interaction Studies With White Spot Syndrome Virus (mentioning

confidence: 99%

Host and Virus Protein Interaction Studies in Understanding Shrimp Virus Gene Function

Otta

2012

Indian J. Virol.

View full text Add to dashboard Cite

Protein-protein interaction studies have been widely used in several fields to characterize an unknown protein. This in turn helps to find out several pathways to understand a complex mechanism or discover a drug for treatment. Among the methods, yeast two-hybrid has widely been used in human, animal and plant research studies. This aspect of research has also been found useful in understanding the shrimp virus gene function. With respect to White spot syndrome virus, interaction studies have been applied to elucidate virus structure, understand the mode of entry of the virus, mechanism of virus replication and also to discover some of the host anti-viral proteins. Interaction studies on other shrimp viruses are scanty and only few reports available on Yellow head virus and Taura syndrome virus. All these findings are still in preliminary stage and lot more studies are necessary to have the clear picture. Protein interaction research on other shrimp viruses are still lacking. Considering all these, it appears that this field of research has a wide scope to understand the virulence mechanism of shrimp viruses where very little information is available till date.

show abstract

Effect of VP28 DNA vaccine on white spot syndrome virus in Litopenaeus vannamei

Liu

Lin

et al. 2010

Aquacult Int

View full text Add to dashboard Cite

White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. Considering the global environmental, the economic and sociological importance of shrimp farming, and the constraints of high intensity cultivation, development of novel control measures against the outbreak of WSSV become inevitable. In this study, we have explored the protective efficacy of DNA vaccination and tissue distribution of the recombinant plasmid in immunized Litopenaeus vannamei. The VP28 gene was cloned in the eukaryotic expression vector pVAX1, and the construct vector was named as lpv28. The protective effect of lpv28 against WSSV was evaluated in L. vannamei by injecting lpv28 construct and later challenging with WSSV. Expression of these proteins from the recombinant plasmids was confirmed in vitro by RT-PCR and Western blot analysis. The result of vaccination trials showed that a survival rate in shrimp vaccinated with lpv28 was 52.5% at most compared to control groups (100% mortality). The immunological parameters analyzed in the vaccinated and control groups showed that the vaccinated groups owned a high level of lysozyme, alkaline phosphatase, and total superoxide dismutase when compared to the control group. Furthermore, protein expression analysis indicated that VP28 can be detected in gill, muscle and head soft tissue of the shrimps in the immunized group after 14th day injection. Thus, the result indicated that DNA vaccination strategy has a potential utility against WSSV.

show abstract

White spot syndrome virus VP37 interacts with VP28 and VP26

Cited by 27 publications

References 26 publications

VP292 of White spot syndrome virus Interacts with VP26

VP292 of White spot syndrome virus Interacts with VP26

Host and Virus Protein Interaction Studies in Understanding Shrimp Virus Gene Function

Effect of VP28 DNA vaccine on white spot syndrome virus in Litopenaeus vannamei

Contact Info

Product

Resources

About