White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District

Abstract

Vaname shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture Vaname shrimp (L. vannamei) traditionally. The occurrence of a decrease in production due to White Spot Syndrome Virus (WSSV) virus attacks that cause a lot of losses for farmers, thus it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp (L. vannamei) farms in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds sites). Detection of WSSV was using Nested PCR with shrimp bodyparts taken are swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs, i.e. Bangil 0-15%, Kraton 0% and Rejoso 0-15%.

Challenges and prospects of Viral Envelope protein VP28‐based control strategies to combat white spot syndrome virus in penaeid shrimps: a review

“…WSSV was reported from different geographical regions such as China, Japan, Taiwan, Bangladesh, Thailand, Indonesia, Saudi Arabia, Africa, America and India (Selvin 2010;Jory & Cabrera 2012;Alam 2016). Having an extensive geospatial distribution, WSSV has a broader host range of as many as 43 arthropod species, either hosts or carriers includes shrimp, crayfish, crabs and lobsters (Meng et al 2009;Pazir et al 2011;Usman & Andayani 2018). WSSV is known to infect cells of ectodermal and mesodermal origin (van Hulten et al 2000;Afsharnasab et al 2009).…”

“…2009; Pazir et al . 2011; Usman & Andayani 2018). WSSV is known to infect cells of ectodermal and mesodermal origin (van Hulten et al 2000; Afsharnasab et al .…”