White spot syndrome virus (WSSV) prevalence associated with disease resistance among wild populations of black tiger shrimp,<i>Penaeus monodon</i>(Fabricius)

Dutta, Sourav; Chakrabarty, Usri; Mallik, Ajoy; Mandal, Nripendranath

doi:10.1111/are.12193

Cited by 12 publications

(10 citation statements)

References 32 publications

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“…Dutta et al . () reported the usefulness of real‐time PCR for the detection of WSSV at low viral load and also predicted the possibility of detection of WSSV infection at initial stage and could take immediate preventive measure of WSSV in cultured shrimp. Therefore, real‐time PCR may be used to detect WSSV‐infected PL of shrimp before stocking to the farm.…”

Section: Resultsmentioning

confidence: 96%

“…The gross clinical sign of this disease is the appearance of white spots on cuticle, and as a result, rapid reduction in food consumption and abnormal movement appear (Dutta et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…WSSV has a wide host range such as shrimps, prawn, crabs and crayfish, and transmission occurs both vertically from infected mother shrimp to postlarvae (PL) and horizontally through water, through live feed Artemia and by cannibalism of moribund shrimp (S anchez-Paz 2010). The gross clinical sign of this disease is the appearance of white spots on cuticle, and as a result, rapid reduction in food consumption and abnormal movement appear (Dutta et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Hossain

Nandi

Siddique

et al. 2014

Lett Appl Microbiol

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White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the coastal regions in Bangladesh. A total of 60 shrimp samples, collected from the 25 shrimp farms of different coastal regions (Satkhira, Khulna, Bagerhat and Cox's Bazar), were analysed during 2013-2014 by conventional PCR using VP28 and VP664 gene-specific primers; 39 of 60 samples were found WSSV positive. SYBR green real-time PCR using 71-bp amplicon for VP664 gene correlated well with conventional PCR data. The prevalence rates of WSSV among the collected 60 samples were Satkhira 79%, Khulna 50%, Bagerhat 38% and Cox's Bazar 25%. Sequencing of WSSV-positive PCR amplicons of VP28 showed 99% similarity with WSSV NCBI Ref/Seq Sequences. Molecular analysis of the VP28 gene sequences of WSSV revealed that Bangladeshi strains phylogenetically affiliated to the strains belong to India. This work concluded that WSSV infections are widely distributed in the coastal regions cultured shrimp in Bangladesh.

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Section: Resultsmentioning

confidence: 96%

“…The gross clinical sign of this disease is the appearance of white spots on cuticle, and as a result, rapid reduction in food consumption and abnormal movement appear (Dutta et al . ).…”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence and distribution of W hite S pot S yndrome V irus in cultured shrimp

Hossain

Nandi

Siddique

et al. 2014

Lett Appl Microbiol

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“…Previously, for the first time in P. monodon , a 71 bp microsatellite DNA marker was reported to be significantly present in disease‐susceptible shrimps (Mukherjee and Mandal, ). Later on, WSSV challenge experiment also showed that 1.21 × 10 3 fold higher WSSV propagation occurred in disease‐susceptible shrimps establishing the significant link between this marker and disease susceptibility (Dutta et al., ). Additionally, a 457 bp DNA marker developed by RAPD‐SCAR method was also found in P. monodon , which is mostly present in the disease‐resistant shrimp.…”

Section: Introductionmentioning

confidence: 99%

Study on the Distribution of Disease-Resistant Shrimp Identified by DNA Markers in Respect to WSSV Infection in Different Seasons Along the Entire East Coast of India Aiming to Prevent White Spot Disease inPenaeus monodon

Mallik

Chakrabarty

Dutta

et al. 2014

Transbound Emerg Dis

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White spot disease caused by white spot syndrome virus (WSSV) is responsible for harming shrimp aquaculture industry and results in a pandemic throughout the world. Undeniably, the knowledge on geographic distribution, transmission, virulence, and seasonal prevalence of this disease alongside information on the distribution of disease-resistant shrimps may be helpful to understand important aspects of disease biology. This study was intended to estimate WSSV prevalence by qualitative and quantitative PCR method among the Penaeus monodon samples collected from four different places namely Digha, West Bengal; Chilika, Orissa; Visakhapatnam, Andhra Pradesh; and Chennai, Tamil Nadu at three different seasons in the period of 2011-2013 from east coast of India. Along with this, the disease-resistant prevalence was also investigated using earlier developed 71 bp microsatellite and 457 bp RAPD-SCAR DNA marker among the collected shrimps. Qualitative PCR depicted that the cumulative WSSV prevalence at four places was the lowest (0%) at pre-monsoon, whereas, it was the highest (21.2%) during post-monsoon season. Quantitative real-time PCR showed the average copy number of WSSV to be the highest (~10(3) copy μg(-1) shrimp genomic DNA) at post-monsoon season. Additionally, estimated disease-resistant prevalence was the highest in Visakhapatnam (79%) and lowest in Digha (21%). It is well known to all that a trait cannot be identified using a single genetic pattern. This study will significantly contribute insight to develop specific pathogen-resistant (SPR) seeds of P. monodon simultaneously using two DNA markers that would be a cost-effective and safer approach towards disease prevention instead of conventional trends of seed generation from unselected wild broodstock.

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“…The prevalence of WSSV has been reported widely among wild and cultured shrimp. It has been reported that the WSSV prevalence in wild Penaeus monodon was high (56.2%) in Chennai, Tamil Nadu followed by Digha, West Bengal (10.9%), and Visakhapatnam, Andhra Pradesh (0.6%) in India (Dutta et al, 2013). In another study, the prevalence of WSSV was reported to be only about 3.6% in wild invertebrates such as crabs, blue, white and brown shrimps and vertebrates in the environment surrounding shrimp farms along the Pacific coast of Mexico (Macías-Rodríguez et al, 2014).…”

Section: Introductionmentioning

confidence: 99%