White Tea (Camellia sinensis Kuntze) Exerts Neuroprotection against Hydrogen Peroxide-Induced Toxicity in PC12 Cells

Abstract

Tea is a popular beverage whose consumption is associated with prevention of certain disorders. The objective of the study was to investigate the potential neuroprotective effect of white tea extract (WTE) on hydrogen peroxide induced toxicity in PC12 cells. Cells were treated with various doses of WTE (10-250 μg/ml) before exposition to 250 μM hydrogen peroxide and cell survival was determined through the MTT and LDH assays. Oxidative stress was quantified in the cells after treatments as intracellular reactive oxygen species (ROS) production and the antioxidant activity of the extract was assessed in a cell free system in terms of free radical scavenging capacity. Results showed that WTE has a significant protective effect in the PC12 cell line against hydrogen peroxide as cell survival was significantly superior in WTE-treated cells compared to hydrogen peroxide-treated cells. A reduction on intracellular oxidative stress as well as radical scavenging properties were produced by WTE. Results suggest that WTE protects PC12 cells against H(2)O(2)-induced toxicity, and that an antioxidant mechanism through ROS scavenging may be in part responsible for cells neuroprotection.

Antioxidant, antihemolytic, and inhibitory activities of endemicPrimula heterochromaagainst Fe2+-induced lipid peroxidation and oxidative stress in rat brainin vitro

“…Previous reports exhibit that electron donor materials can scavenge DPPH radicals by electron or hydrogen donation and change the color of solutions from violet to yellow (López & Calvo, 2011). Iron chelation therapy is a consequential method to diminish iron ion in iron mediated diseases and so improve life quality and generally survival in different iron mediated diseases such as thalassemia major.…”

“…For example, in Parkinson disease, chelated iron cannot contribute to oxidative stress and restrain dopaminergic midbrain neurons decay (Jomova et al, 2010). In DPPH and iron chelating models, aqueous fractions show better activity than other fractions and may correlate with its higher phenolic content (López & Calvo, 2011).…”

White tea (Camellia sinensis) extract reduces oxidative stress and triacylglycerols in obese mice

“…tea, white tea has been used to reduce oxidative stress and to treat obesity and associated diseases. However, most studies using white tea investigated its composition (ALCAZAR et al, 2007;MALINOWSKA et al, 2008;UNACHUKWU et al, 2010; VAN DER HOOFT et al, 2012), its antitumorigenic effect (ANGER; PETRE; CRANKSHAW, 2005;KUMAR et al, 2012;WANG et al, 2008), or its antioxidant action (ALMAJANO; VILA; GINES, 2011;CALZUOLA;GIANFRANCESCHI;MARSILI, 2006;KOUTELIDAKIS et al, 2009;LOPEZ;CALVO, 2011;PEREZ-JIMENEZ et al, 2011;SEERAM et al, 2008;THRING;HILI;NAUGHTON, 2009NAUGHTON, , 2011. Nonetheless, despite its popular use, there are no in vivo studies relating white tea and obesity associated with oxidative stress.…”

White tea (Camellia sinenesis (L.)) ethanol extracts attenuate reserpine-induced gastric ulcers in mice

“…Generally, tea products are divided into non-fermented tea, including green and white tea, partially fermented tea, such as oolong tea, and completely fermented black tea. White tea is prepared from the young leaves and buds of the C. sinenesis plant plucked only during the early spring before buds are fully opened (15). White tea contains higher levels of tea polyphenols, caffeine, and catechins, including epigallocathechins (EGC), epigallocatechins gallate (EGCG), epicatchin gallate (ECG), and epicatchin gallate (ECG) than green tea.…”