Whole blood fatty acid composition at birth: From the maternal compartment to the infant

Agostoni, Carlo; Galli, Claudio; Riva, Enrica; Risé, Patrizia; Colombo, Claudio; Giovannini, Marcello; Marangoni, Franca

doi:10.1016/j.clnu.2011.01.016

Cited by 24 publications

(15 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transfer of DHA across the placenta involves specific binding and transfer proteins facilitating the higher concentration of DHA in fetal compared with maternal plasma (4). AA-concentrations are also higher in the fetus, whereas concentrations of linoleic acid (LA) and α-linolenic acid (ALA) in maternal and infant blood differ much less (5,6). The phenomenon of increasing LCPUFA in the fetal department has been described as "biomagnification" (7), but could also be interpreted as a natural consequence of a dual liver system (i.e., the combined PUFA-metabolism of the mother and the fetus/infant).…”

Section: Maternal-fetal Transfer Of Arachidonic Acidmentioning

confidence: 99%

Dietary arachidonic acid in perinatal nutrition: a commentary

Lauritzen

Fewtrell²,

Agostoni

2014

Pediatr Res

Self Cite

View full text Add to dashboard Cite

Section: Maternal-fetal Transfer Of Arachidonic Acidmentioning

confidence: 99%

Dietary arachidonic acid in perinatal nutrition: a commentary

Lauritzen

Fewtrell²,

Agostoni

2014

Pediatr Res

Self Cite

View full text Add to dashboard Cite

“…3). In addition, microwaveassisted transesterification yields fatty acid concentrations 4 Fatty acid methyl ester (FAME) yield from 200 nmol sphingomyelin standard following direct transesterification with 1 % sulfuric acid in methanol. *Indicates FAME significantly different than 3-h control values as determined by Tukey's HSD post hoc test following a significant F value by One-way ANOVA.…”

Section: Microwave-assisted Transesterification Of Isolated Sphingomymentioning

confidence: 99%

“…Dried blood spots (DBS) collected by fingertip prick provide fatty acid determinations similar to venous whole blood sampling [1,2], and in recent years have gained in acceptance and been adopted for use in a variety of populations including infants [3,4], the elderly [5] and military personnel [6], among others [2,[7][8][9]. In addition, the % sum of eicosapentaenoic acid (EPA, 20:5n-3) + docosahexaenoic acid (DHA, 22:6n-3) in whole blood can be used as a predictor for risk of sudden cardiac death [10].…”

Section: Introductionmentioning

confidence: 99%

Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Metherel

Henao

Ciobanu

et al. 2015

Lipids

View full text Add to dashboard Cite

Dried blood spots (DBS) by fingertip prick collection for fatty acid profiling are becoming increasingly popular due to ease of collection, minimal invasiveness and its amenability to high‐throughput analyses. Herein, we assess a microwave‐assisted direct transesterification method for the production of fatty acid methyl esters (FAME) from DBS. Technical replicates of human whole blood were collected and 25‐μL aliquots were applied to chromatography strips prior to analysis by a standard 3‐h transesterification method or microwave‐assisted direct transesterification method under various power (variable vs constant), time (1–5 min) and reagent (1–10 % H2SO4 in methanol) conditions. In addition, a standard method was compared to a 5‐min, 30‐W power microwave in 1 % H2SO4 method for FAME yield from whole blood sphingomyelin, and sphingomyelin standards alone and spiked in whole blood. Microwave‐assisted direct transesterification yielded no significant differences in both quantitative (nmol/100 µL) and qualitative (mol%) fatty acid assessments after as little as 1.5‐ and 1‐min reaction times, respectively, using the variable power method and 5 % H2SO4 in methanol. However, 30‐W power for 5 min increased total FAME yield of the technical replicates by 14 %. This increase appears largely due to higher sphingomyelin‐derived FAME yield of up to 109 and 399 % compared to the standard method when determined from whole blood or pure standards, respectively. In conclusion, microwave‐assisted direct transesterification of DBS achieved in as little as 1‐min, and 5‐min reaction times increase total fatty acids primarily by significantly improving sphingomyelin‐derived fatty acid yield.

show abstract

“…However, they do not only reflect intakes of n-3 LCPUFAs during pregnancy, but also their absorption, metabolism and incorporation into plasma fractions, cells or tissues (which is determined by genetic background and health/disease status), as well as the intakes of other fatty acids in the diet. In addition, at the end of pregnancy, a general decline in n-3 LCPUFAs takes place in the maternal compartment, which is largely independent of differences in dietary habits and ethnic origin (Otto et al, 1997), together with a progressive transfer of maternal n-3 LCPUFAs to the umbilical cord and then the infant's blood (Agostoni et al, 2011). Thus, markers of maternal or cord blood n-3 LCPUFA may reflect only in part intakes of seafood during pregnancy (Silva et al, 2014).…”

Section: Biomarkers Of N-3 Lcpufamentioning

confidence: 99%

Scientific Opinion on health benefits of seafood (fish and shellfish) consumption in relation to health risks associated with exposure to methylmercury

Products¹

2014

EFSA Journal

136

View full text Add to dashboard Cite

Following a request from the European Commission to address the risks and benefits as regards fish/seafood consumption related to relevant beneficial substances (e.g. nutrients such as n‐3 long‐chain polyunsaturated fatty acids) and the contaminant methylmercury, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a Scientific Opinion on health benefits of seafood consumption in relation to health risks associated with exposure to methylmercury. In the present Opinion, the NDA Panel has reviewed the role of seafood in European diets and evaluated the beneficial effects of seafood consumption in relation to health outcomes and population subgroups that have been identified by the FAO/WHO Joint Expert Consultation on the Risks and Benefits of Fish Consumption and/or the EFSA Panel on Contaminants in the context of a risk assessment related to the presence of mercury and methylmercury in food as relevant for the assessment. These included the effects of seafood consumption during pregnancy on functional outcomes of children's neurodevelopment and the effects of seafood consumption on cardiovascular disease risk in adults. The Panel concluded that consumption of about 1‐2 servings of seafood per week and up to 3‐4 servings per week during pregnancy has been associated with better functional outcomes of neurodevelopment in children compared to no consumption of seafood. Such amounts have also been associated with a lower risk of coronary heart disease mortality in adults and are compatible with current intakes and recommendations in most of the European countries considered. These associations refer to seafood per se andinclude beneficial and adverse effects of nutrients and non‐nutrients (i.e. including contaminants such as methylmercury) contained in seafood. No additional benefits on neurodevelopmental outcomes and no benefit on coronary heart disease mortality risk might be expected at higher intakes.

show abstract

Whole blood fatty acid composition at birth: From the maternal compartment to the infant

Cited by 24 publications

References 9 publications

Dietary arachidonic acid in perinatal nutrition: a commentary

Dietary arachidonic acid in perinatal nutrition: a commentary

Microwave Energy Increases Fatty Acid Methyl Ester Yield in Human Whole Blood Due to Increased Sphingomyelin Transesterification

Scientific Opinion on health benefits of seafood (fish and shellfish) consumption in relation to health risks associated with exposure to methylmercury

Contact Info

Product

Resources

About