2015
DOI: 10.4014/jmb.1408.08059
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Whole-Blood Gene-Expression Profiles of Cows Infected with Mycobacterium avium subsp. paratuberculosis Reveal Changes in Immune Response and Lipid Metabolism

Abstract: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic debilitating disease affecting ruminants worldwide. In the present study, we aimed to determine the major gene networks and pathways underlying the immune response to MAP infection using whole-blood cells, as well as provide the potential transcriptional markers for identifying the status of MAP infection. We analyzed the transcriptional profiles of whole-blood cells of cattle identified and grouped according… Show more

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Cited by 28 publications
(31 citation statements)
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“…JD manifests several stages of infection, such as silent, subclinical, clinical, and advanced cell infections, depending on the symptom evaluation and quantification of intestinal MAP (11,12). Additionally, the progression of mycobacterial infection has been thought to be related to Th1 / Th2 migration of the host (13).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…JD manifests several stages of infection, such as silent, subclinical, clinical, and advanced cell infections, depending on the symptom evaluation and quantification of intestinal MAP (11,12). Additionally, the progression of mycobacterial infection has been thought to be related to Th1 / Th2 migration of the host (13).…”
Section: Discussionmentioning
confidence: 99%
“…Th1 response decreases as the disease progresses, but the antibody response (Th2 response) to MAP increases (13). Thus, since the Th1 / Th2 shift is related to disease progression and bacterial excretion, many studies have determined the MAP infection stage based on detection of MAP and MAP-specific antibody in specimen such as feces, milk, and sera (12,14,15). DNA-detected with ELISA-negative, and was expected to be detectable at the early state of MAP infection.…”
Section: Discussionmentioning
confidence: 99%
“…Our previous studies also proposed several biomarkers that were up-regulated in MAP infected macrophages, mice, and cattle [21][22][23]. Transcriptional profiles of MAP-infected macrophage RAW 264.7 cells and a mouse model suggested five and three genes as prognostic biomarkers, respectively [21,22]. β-defensins were also suggested as prognostic biomarkers in subclinical animals of MAP-naturally infected cattle [23].…”
Section: Introductionmentioning
confidence: 91%
“…Moreover, six proteins (transferrin, gelsolin isoforms α & β, complement subcomponent C1r, complement component C3, amine oxidasecopper containing 3, and coagulation factor II) were proposed as biomarkers after they were found to increase by at least 2-fold in MAP-infected cattle, as were two proteins (coagulation factor XIII-B polypeptide, and fibrinogen γ chain and its precursor) that were reduced by nearly two-fold in MAP-infected cattle [20]. Our previous studies also proposed several biomarkers that were up-regulated in MAP infected macrophages, mice, and cattle [21][22][23]. Transcriptional profiles of MAP-infected macrophage RAW 264.7 cells and a mouse model suggested five and three genes as prognostic biomarkers, respectively [21,22].…”
Section: Introductionmentioning
confidence: 95%
“…Using proteomic and transcriptomic analyses, several putative biomarkers for early infection with MAP have been proposed (Casey et al., ; David, Barkema, Luo Guan, & De Buck, ; David, Barkema, Mortier, et al., ; Seth et al., ; Shin et al., ; Skovgaard et al., ; Thirunavukkarasu et al., ; You et al., ; Zhong, Taylor, Begg, & Whittington, ). Metabolomic profiling detected MAP infection earlier than other diagnostic methods, with individual metabolites distinguishing infected from non‐infected cattle (De Buck, Shaykhutdinov, Barkema, & Vogel, ).…”
Section: Testsmentioning
confidence: 99%