Whole Blood Glucose: What are We Actually Measuring?

Wiener, K

doi:10.1177/000456329503200101

Cited by 51 publications

(16 citation statements)

References 26 publications

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“…The mean (95% confidence interval) is -0·66 (-0'24 to -1'08)mmol/L, respectively, and limits and agreement are -3·8 to + 2·5 mmol/L, Mean blood glucose varied between 5·5-6·4mmol/L in the haematocrit range 0·25-D·64 and from 18,5-21,1 mrnol/L within the haematocrit range 0·24-D·57. At sample volumes of 9, 8, 7 and 6 ilL, mean glucose levels of 16,6, 15·7, 17·1, 17'Ommol/L and 4·5,[3][4][3][4] and 3·9 mrnol/L respectively were recorded on the two samples tested. In both cases totally discrepant results were obtained below 6 J.LL, although an error message did not appear until 3 J.LL was applied.…”

Section: Resultsmentioning

confidence: 99%

An Assessment of the Boehringer Advantage Blood Glucose Meter

1997

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Additional key phrases: blood glucose: Advantage meterBlood glucose meters have been in use in the North Down and Ards Hospital Trust for the last 8 years. Our initial assessments'< had shown the Boehringer Reflolux 11 to be the instrument of choice. Since then the Reflolux 11 has been replaced by the Boehringer Accutrend. Although this instrument was found to be reliable when used correctly.l in practice we have found problems due to insufficient sample being applied to the strip or blood collecting on the inside of the instrument. The Advantage glucose meter appears to overcome these difficulties. METHODSThe Advantage glucose meter is a small hand held battery powered meter using dry chemistry reagent strips containing electrodes. Each vial of strips is coded and contains a calibration key which is snapped into place in the back of the meter before use. The number which then flashes on the instrument corresponds to the number on the side of the vial. The strip is inserted into the meter leaving a yellow coloured test window exposed. A minimum 9 J.LL blood sample applied to this area ensures no yellow is visible. The reaction is based on the oxidation of blood glucose by glucose dehydrogenase linked to reduction of ferri-to ferrocyanide. Electrons generated in this reaction pass down the electrodes and are measured in the instrument. The read time is 40 s and the stated range is 0·56-33·3mmol/L. The manufacturers state that results are not affected by haematocrit in the range 20--65% at a glucose level < 11·1 mmol/L and 25-55% at glucose levels> 11·1 mmol/L or by bilirubin up to a level of 342 J.Lmol/L. The instrument can operate capillary, venous fluoride oxalate, heparin but not ethylene-diaminetetraacetic acid (EDTA) samples.Precision was determined in the laboratory by replicate analysis of two venous, fluoride oxalate blood samples with medium and high levels of The effect of haematocrit was studied using two fluoride oxalate samples with blood glucose levels of 5·9 and 21·1 mmol/L, These were each centrifuged to separate plasma from cells and then recombined in different proportions to give blood samples with a haematocrit range of 0·09--64 and 0·09-D·58, respectively, on each of which blood glucose was measured in duplicate.Minimum sample volume was studied using decreasing amounts (9-2 J.LL) of fluoride oxalate blood samples with medium and high levels of blood glucose assayed in duplicate to determine at what level an error message appeared.Interference from bilirubin was studied using a reconstituted bilirubin standard (Sigma Chemical Co Ltd, Poole, Dorset BHI7 7BR, UK). RESULTSThe within batch coefficients of variation at the blood glucose levels of 0,98, 1'9, 6·6 and 21·1 mmol/L were 18-4 (n= 12), 8·1 (n= 12), 3·5 (n=20) and 4·1% (n = 20), respectively.A difference plot! of the Apec and Advantage is given in Fig. 1. The mean (95% confidence interval) is -0·66 (-0'24 to -1'08)mmol/L, respectively, and limits and agreement are -3·8 to + 2·5 mmol/L, Mean blood glucose varied between 5·5-6·4mmol/L in t...

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Section: Resultsmentioning

confidence: 99%

An Assessment of the Boehringer Advantage Blood Glucose Meter

1997

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“…The instrument is calibrated using an aqueous glucose standard solution. 35 Similar to measurements obtained by PBGMs, hematocrit may affect glucose values obtained by this method. 53…”

Section: Portable Blood Glucose Metersmentioning

confidence: 67%

“…When a drop of blood is applied to the surface, plasma soaks through into the reagent layer and red cells are retained on the surface. 35 Depending on the particular device, it may use colorimetric or amperometric detection systems to measure glucose concentration.…”

Section: Portable Blood Glucose Metersmentioning

confidence: 99%

Blood Glucose Monitoring and Glycemic Control

Reineke¹

2012

Advanced Monitoring and Procedures for Small Animal Emergency and Critical Care

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“…Molality is the same in whole blood or plasma; thus, methods based on measuring activity provide equivalent results with either specimen type. 20,21 In contrast, methods based on measuring concentration (amount of glucose per unit specimen volume) do not provide equivalent results because there is more water in plasma than in erythrocytes. Likewise, it is important to keep in mind that measurements of concentration depend on the assumption that the amount of water in the calibrators and in the specimens are identical.…”

Section: Current Issues Associated With Point-of-care Testing Measurementioning

confidence: 99%

Bedside Blood Glucose Testing Systems

Sluss¹

2006

Point of Care: The Journal of Near-Patient Testing &Amp; Technology

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Bedside glucose monitoring is an essential component of maintaining blood glucose control in patients. A wide range of devices designed for point-of-care testing or home use are currently available for bedside monitoring of blood glucose levels. This article outlines the principles of blood glucose measurement by such devices, which are designed on the basis of the action of glucose-digesting enzymes. An appreciation of the mechanisms that underlie enzyme systems for measuring glucose is important in the selection and use of bedside glucometers.

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Whole Blood Glucose: What are We Actually Measuring?

Cited by 51 publications

References 26 publications

An Assessment of the Boehringer Advantage Blood Glucose Meter

An Assessment of the Boehringer Advantage Blood Glucose Meter

Blood Glucose Monitoring and Glycemic Control

Bedside Blood Glucose Testing Systems

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