Whole blood platelet aggregation determined by the ROTEM platelet equipment; reference intervals and stability

Nissen, Peter H.; Skipper, Mette Tiedemann; Hvas, Anne‐Mette

doi:10.1080/09537104.2019.1595562

Cited by 17 publications

(19 citation statements)

References 25 publications

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“…AUC reflects the overall platelet aggregation. 19,20 Recent studies have investigated the capability of different types of POC platelet function tests to predict the risk of postoperative bleeding; a nonstandardized approach in study design and reporting results, coupled with lack of consensus in definition of observed outcomes and various devices used, makes it difficult to pool the evidence on the exact role of POC platelet function testing in routine hemostatic management. We will assess platelet function with three activators at two different time points: before surgery and after heparin reversal with protamine and evaluate whether perioperative platelet function, as assessed by impedance aggregometry at two different time points, may reflect bleeding measured as the volume of postoperative chest tube drainage.…”

Section: Discussionmentioning

confidence: 99%

Role of Platelet Function Test in Predicting Postoperative Bleeding Risk after Coronary Artery Bypass Grafting: A Prospective Observational Study

Sharan

Kapoor

Choudhury

et al. 2021

Journal of Cardiac Critical Care TSS

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Patients undergoing cardiac surgery are at risk of excessive bleeding and its associated complications. Excessive bleeding during and after cardiac surgery has an incidence of ~20%. Massive bleeding and subsequent requirement for blood product administration and mediastinal reexploration are associated with significant morbidity and mortality. Postoperative, nonsurgical bleeding in cardiac surgical patients is often multifactorial. Platelet dysfunction, excessive fibrinolysis, hypothermia, preoperative anemia, and deficiency of coagulation factors or their dilution are all suggested etiologies of postoperative bleeding. In the Arachidonic Acid Thromboelastometry (ARATEM) test, platelets are activated with arachidonic acid; in Adenosine diphosphate Thromboelastometry (ADPTEM) test, platelets are activated with adenosine diphosphate; and in TRAPTEM test, platelets are activated with thrombin receptor-activating peptide 6. Measurement time is 6 minutes, and results are expressed in three different parameters: A6 (amplitude at 6 minutes, in Ohm); MS (maximum slope of the aggregation curve in Ohm/min), and AUC (area under the curve in Ohm.min). Algorithm-based point-of-care platelet function testing helped us to preemptively give the right blood component therapy, avoiding fibrinolytic bleeding in the postoperative period.

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Section: Discussionmentioning

confidence: 99%

Role of Platelet Function Test in Predicting Postoperative Bleeding Risk after Coronary Artery Bypass Grafting: A Prospective Observational Study

Sharan

Kapoor

Choudhury

et al. 2021

Journal of Cardiac Critical Care TSS

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show abstract

“…Several previous studies reported a trend toward hypercoagulability in females compared with males, [27] and part of this phenomenon could be addressed by higher PLT values and platelet aggregation in females than males. [23,28] However, these studies were undertaken in adults and older children population. Just a few studies investigated gender differences in neonatal coagulation.…”

Section: Discussionmentioning

confidence: 99%

Capillary blood reference intervals for platelet parameters in healthy full-term neonates in China

Cui

Hou

Feng

et al. 2020

Preprint

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Background No consensus has been reached on reference intervals for platelet parameters in full-term neonates. We aimed to establish neonatal capillary blood reference intervals for platelet parameters and evaluate influences of gender, gestational age and postnatal age on platelet parameters. Methods This study was implemented in 594 healthy full-term neonates from 12 to 84 hours of age, using SYSMEX XN-9000 haematology automatic analyser by means of capillary blood. Reference intervals for platelet parameters were defined by an interval of 2.5th − 97.5th percentiles. Results Capillary reference interval for platelet count was (152–464) × 109/L. No significance was found between gender-divided reference intervals for platelet parameters. The values of platelet count changed significantly across gestational age and postnatal age. Reference intervals for other platelet parameters were affected by these factors to a different extent. Conclusions We established capillary blood reference intervals for platelet parameters in the early phases after birth of full-term neonates in China for the first time.

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“…Sample storage times were chosen based on the available literature as well as clinical and logistic reasons. 24,29,34,36 Exact times varied ± 3 min—depending on order of analyses—given the time needed to perform each test step. At each sample storage time, analyses with ara-tem and adp-tem were performed in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Whole blood platelet impedance aggregometry with the ROTEM platelet device: comparison of 2 anticoagulants and storage times for the establishment of canine reference intervals

et al. 2021

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The ROTEM platelet device, a point-of-care whole blood platelet impedance aggregometer, is an add-on to the rotational thromboelastometry ROTEM delta device. The latter has been validated in dogs. We examined whether canine whole blood is suited for analysis with the ROTEM platelet device using adenosine-5′-diphosphate (ADP) and arachidonic acid (ARA) as agonists for platelet activation, and if there are significant differences between sample storage times and anticoagulants used. Subsequently, we determined canine reference intervals (RIs) for the ROTEM platelet device for ADP and ARA. In a pilot study, we examined whole blood from 7 dogs after 15-min and 60-min storage of lithium-heparinized samples and 40-min and 80-min storage of hirudinized samples. Statistical analysis showed no significant differences between ROTEM platelet device results for both ADP and ARA in lithium-heparin and hirudin anticoagulated canine whole blood. Lithium-heparinized blood samples analyzed after 15-min storage had the lowest coefficient of variation. RIs were determined for heparinized whole blood samples from 49 dogs after 15 min of storage.

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Whole blood platelet aggregation determined by the ROTEM platelet equipment; reference intervals and stability

Cited by 17 publications

References 25 publications

Role of Platelet Function Test in Predicting Postoperative Bleeding Risk after Coronary Artery Bypass Grafting: A Prospective Observational Study

Role of Platelet Function Test in Predicting Postoperative Bleeding Risk after Coronary Artery Bypass Grafting: A Prospective Observational Study

Capillary blood reference intervals for platelet parameters in healthy full-term neonates in China

Whole blood platelet impedance aggregometry with the ROTEM platelet device: comparison of 2 anticoagulants and storage times for the establishment of canine reference intervals

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