Whole blood pumping with a microthrottle pump

Abstract

We have previously reported that microthrottle pumps (MTPs) display the capacity to pump solid phase suspensions such as polystyrene beads which prove challenging to most microfluidic pumps. In this paper we report employing a linear microthrottle pump (LMTP) to pump whole, undiluted, anticoagulated, human venous blood at 200 μl min(-1) with minimal erythrocyte lysis and no observed pump blockage. LMTPs are particularly well suited to particle suspension transport by virtue of their relatively unimpeded internal flow-path. Micropumping of whole blood represents a rigorous real-world test of cell suspension transport given blood's high cell content by volume and erythrocytes' relative fragility. A modification of the standard Drabkin method and its validation to spectrophotometrically quantify low levels of erythrocyte lysis by hemoglobin release is also reported. Erythrocyte lysis rates resulting from transport via LMTP are determined to be below one cell in 500 at a pumping rate of 102 μl min(-1).

Curing Temperature Effects on the Tensile Properties and Hardness of γ −Fe2O3 Reinforced PDMS Nanocomposites

“…Notably, the ability of PDMS to undergo relatively large elastic deformation has been exploited for the development of several theranostic biomedical applications such as lab/tissue/organs-on-chip devices, point-of-care devices, and two/three-dimensional cell culture [3,4,9]. Examples include micropumps employing elastomeric displacement amplification [10,11], cell-based biochips using elastomeric substrates [12,13], and microfluidic channel for pressure monitoring [14,15].…”

Determining thermo‐mechanical properties of polydimethylsiloxanes from their strain‐induced spectral fingerprints

“…Polydimethylsiloxane (PDMS) has a wide range of applications ranging from mechanical, microfluidic, biomedical applications, and various other applications . The wide use of PDMS for various fields is due to its tunable mechanical properties that vary up to three orders of magnitude .…”

A “place n play” modular pump for portable microfluidic applications

“…1932-1058/2012/6(1)/014118/16/$30.00 V C 2012 American Institute of Physics 6, 014118-1 microfluidic applications by utilizing piezoelectric, [11][12][13][14] electrostatic, 15 pneumatic, 16,17 or magnetic effects. 18 Although these on-chip pumps increase the device portability, integrating the pumping components (e.g., diaphragms, actuators, valves, and heaters) on the device significantly increases the fabrication complexity and device cost.…”