Whole Blood Transcriptome Analysis Reveals the Correlation between Specific Immune Cells and Septicemic Melioidosis

Xu, Ke; Xu, Dahua; Peng, Hua; Quan, Yunfan; Li, Jun; Li, Yin; Li, Xuexia; ShenTian,; Li, Kongning; Xia, Qianfeng

doi:10.1155/2021/6166492

Cited by 1 publication

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“…Many patients with melioidosis have abnormal immune conditions, suggesting that the onset and development of the disease are mainly determined by the host's immune status [ 12 – 14 ]. This has been exemplified by twofold.…”

Section: Introductionmentioning

confidence: 99%

Septicemic Melioidosis Detection Using Support Vector Machine with Five Immune Cell Types

Lian

Quan

et al. 2021

Disease Markers

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Melioidosis, caused by Burkholderia pseudomallei (B. pseudomallei), predominantly occurs in the tropical regions. Of various types of melioidosis, septicemic melioidosis is the most lethal one with a mortality rate of 40%. Early detection of the disease is paramount for the better chances of cure. In this study, we developed a novel approach for septicemic melioidosis detection, using a machine learning technique—support vector machine (SVM). Several SVM models were built, and 19 features characterized by the corresponding immune cell types were generated by Cell type Identification Estimating Relative Subsets Of RNA Transcripts (CIBERSORT). Using these features, we trained a binomial SVM model on the training set and evaluated it on the independent testing set. Our findings indicated that this model performed well with means of sensitivity and specificity up to 0.962 and 0.979, respectively. Meanwhile, the receiver operating characteristic (ROC) curve analysis gave area under curves (AUCs) ranging from 0.952 to 1.000. Furthermore, we found that a concise SVM model, built upon a combination of CD8+ T cells, resting CD4+ memory T cells, monocytes, M2 macrophages, and activated mast cells, worked perfectly on the detection of septicemic melioidosis. Our data showed that its mean of sensitivity was up to 0.976 while that of specificity up to 0.993. In addition, the ROC curve analysis gave AUC close to 1.000. Taken together, this SVM model is a robust classification tool and may serve as a complementary diagnostic technique to septicemic melioidosis.

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Section: Introductionmentioning

confidence: 99%