Whole blood treated with riboflavin and ultraviolet light: quality assessment of all blood components produced by the buffy coat method

Abstract

Treatment of WB with riboflavin and UV before production of components by the BC method shows a negative impact on all three blood components. PLT units produced from PI-treated WB exhibited less damage compared to PLT component treatment.

Efficiency of riboflavin and ultraviolet light treatment against high levels of biofilm‐derived Staphylococcus epidermidis in buffy coat platelet concentrates

“…Therefore, it is not surprising to detect platelet activation after Mirasol treatment. Similar patterns of reduced in vitro PC quality (including platelet degranulation as response to ADP) after Mirasol treatment have been well documented .…”

Redox Proteomics and Platelet Activation: Understanding the Redox Proteome to Improve Platelet Quality for Transfusion

“…To assess the suitability of the UVB/riboflavin treatment for whole blood, the viability of the different components was tested in vitro. Schubert et al found significant deterioration in plasma coagulation factor activation and increased RBC aging in components derived from whole blood that was PI-treated [144]. Interestingly, platelets showed fewer lesions than PI-treated PCs.…”

Hemostatic properties and protein expression profile of therapeutic apheresis plasma treated with amotosalen and ultraviolet A for pathogen inactivation

“…Moreover, if IBS treatment induces discrete protein modifications such as rupture of hydrogen or ionic bonds, these modifications will not be detected on a 2D gel, although they could have a dramatic impact on the function of the protein. To access less abundant proteins, another possibility would be to combine the 2D‐DIGE method with differential off‐gel proteomic approaches like isobaric tagging for relative and absolute quantification (iTRAQ), stable isotope–labeled amino acids, or isotope‐coded affinity tags, which are all based on a differential analysis of labeled peptides and hence are more sensitive than a differential protein analysis …”