Whole-body autoradiographic study on the distribution of (U-14C)galactose in adult, pregnant, newborn and infant mice.

Shimono, Ryuichi

doi:10.1267/ahc.17.461

Cited by 3 publications

(5 citation statements)

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“…Vrba (36), Vrba et al (39) and Shimada et al (28) showed the rapid incorporation of 14C from 14C-glucose into free amino acids in rat and mouse brains. Our present findings may be consistent with the data of these reports and suggest that most of 14C may be taken up into the brain after being converted into glucose in certain organs (30) and then metabolized further into such free amino acids as glutamate, aspartate, GABA, alanine and glycine.…”

Section: Discussionsupporting

confidence: 93%

“…10, Amersham, UK) and corrected values were epressed as autoradiographic density (AD). AD value 0 was represented by the blank of 14C standards (30).…”

Section: Methodsmentioning

confidence: 99%

“…In the previous paper (30), the distribution of radioactive carbon of [U-14C] galactose in mice was investigated by whole-body autoradiography, which was performed before and after 6 % perchloric acid treatment of cryosection.…”

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confidence: 99%

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Whole-body autoradiographic study of (U-14C) galactose in mice and its biochemical analysis.

Shimono

1985

Acta Histochem. Cytochem

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Tissue and organ distribution and metabolic fate of radioactive carbon from [U-14C] galactose in mice were studied by whole-body autoradiography and biochemical analysis.Autoradiography was performed before and after 6 % perchloric acid treatment at various intervals after intravenous injection of [U-14C] galactose. At 5 min after injection, high radioactivities were observed in the kidney, small intestine and liver.The radioactivities in almost all organs were markedly decreased with the acid treatment.At 30 min after injection, the kidney and small intestine had relatively high radioactivities which were retained by the acid treatment.As for the biochemical analysis, at various intervals after intravenous injection of [U-14C] galactose, the mice were decapitated, and the blood, liver, kidney, small and large intestines, myocardium and brain were removed. These organs were fractionized into 6 % perchloric acid-soluble, -insoluble and lipid parts.Total radioactivity (cpm) per gram of wet weight was the highest in the kidney.The incorporation rates of the radioactivity into the acid-soluble fraction were high in all organs at early intervals, while at late intervals, the rates into the acid-insoluble fraction became relatively high in the kidney, small and large intestines.These results were consistent with those obtained from whole-body autoradiography.Acid-soluble fractions of the blood, kidney, liver and brain were analyzed by paper chromatography.The radioactive spots for galactose and glucose were observed in all organs examined, but the changes of radioactive values with the passage of time were different in each organ.Furthermore, the radioactive spots for RGlucose value 0.28 were detected in these organs.Galactose is C-4 epimer of glucose and is derived from lactose. Ingested lactose is absorbed in the small intestine and then hydrolyzed by 3-galactosidase (lactase) into glucose and galactose. It is generally accepted that galactose is conveyed to the liver, where it is converted to glucose by the Leloir pathway (18). In the previous studies, enzyme activities belonging to this pathway have been found in the liver (3, 5, 8, 33), kidney (3, 5, 8, 16, 20, 25, 34), intestine (23), brain (21,32,40) and myocardium (21) etc. On the other hand, it has been reported that galactose is an important constituent of the glycoproteins, glycolipids and mucopolysaccharides (1,4,10,13,14,15,22).

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Section: Discussionsupporting

confidence: 93%

“…10, Amersham, UK) and corrected values were epressed as autoradiographic density (AD). AD value 0 was represented by the blank of 14C standards (30).…”

Section: Methodsmentioning

confidence: 99%

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Whole-body autoradiographic study of (U-14C) galactose in mice and its biochemical analysis.

Shimono

1985

Acta Histochem. Cytochem

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“…10 Amersham International plc.) was also performed by the same method as described above in order to calibrate optical density and radioactivity (30). The optical densities of autoradiographs of untreated and treated sections and the 14C standards were measured with a microdensitometer (PDM-5, Konishiroku Photo Ind., Co., Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…The distribution of various labeled carbohydrates in the mammalian body under normal conditions has been studied in our laboratory (13,14,(29)(30)(31) 46) with the object of clarifying metabolic cooperation of tissues and organs in the body (41). As a series of this study, distribution of labeled L-fucose in mice was investigated.…”

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confidence: 99%