Whole-body cryostimulation impact on blood serum thermal denaturation profiles of cross-country skiers

Abstract

Blood plasma and serum are important diagnostic materials as they clearly reflect an individual’s metabolism. The study has evaluated the effect of whole-body cryostimulation (WBC) on the blood serum of professional cross-country skiers. The experiment involved eight athletes (two women and six men) who underwent a series of ten WBC treatments. Aqueous solutions of human blood serum samples before WBC procedures, after one treatment and after a series of ten treatments were measured by means of differential scanning calorimetry (DSC), a relatively novel diagnostic tool. DSC results showed rather little impact of cryostimulation on heat capacity changes accompanying the process of thermal denaturation of blood serum proteins in elite athletes. However, the statistically significant reduction in the intensity of the serum denaturation transition in its low temperature range has been observed after ten WBC treatments. The results have been interpreted by changes in the serum proteome profile, notably in the ratio of ligated to unligated albumin molecules. As a side result, the relationships between the relative change in body fat mass after ten WBC treatments and the levels of alpha2-globulins and beta2-globulins fractions have been found.

Differential scanning calorimetry reveals that whole-body cryostimulation in cross-country skiers can modify their response to physical effort

“…It should be also noted that none of the participants was overweight. In our earlier study [63], a decrease of % fat content after 10 WBC was found but mainly for participants with the low level of body fat mass.…”

Modification of blood serum DSC profiles by sauna treatments in cross-country skiers during the exercise cycle

“…The thermal denaturation of the proteins contained in the blood sera is observed in serum DSC profile as complex endothermic transition. At first sight, at least two or three peaks can be distinguished in this transition for healthy people, depending on the serum proteome and the solvent used [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. Mean DSC curves of aqueous solutions of human blood sera samples collected from participants of our study at three time points (before sauna treatments, after 1 sauna treatment and after 10 sauna treatments) are shown in Fig.…”

“…Differential scanning calorimetry (DSC) has found wide application in medical and biological sciences [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. Attempts to use the DSC method for clinical purposes have been made for several years [15][16][17][18][19][20][21][22][23][24][25][26][27].…”

“…DSC profiles of blood serum, plasma, synovial fluid, cerebrospinal fluid are very useful because they can be a valuable source of information about alterations in these physiological fluids caused by different diseases or treatments. DSC seems to be a very informative technique also in sports medicine [28][29][30][31][32].…”

Whole-Body Cryostimulation Improves Inflammatory Endothelium Parameters and Decreases Oxidative Stress in Healthy Subjects

“…WBC procedures have a vast scope of application; apart from post-traumatic rehabilitation of injured locomotor system, they are also in the training of professional athletes. A significant factor is a decrease in the intensity of post-traumatic pain and edema, to which athletes are especially exposed during their training, as well as a subjective improvement in general condition and better exercise tolerance [ 2 , 12 , 13 , 14 ].…”