Whole-body multispetral photoacoustic imaging of adult zebrafish

Abstract

The zebrafish, an ideal vertebrate for studying developmental biology and genetics, is increasingly being used to understand human diseases, due to its high similarity to the human genome and its optical transparency during embryonic stages. Once the zebrafish has fully developed, especially wild-type breeds, conventional optical imaging techniques have difficulty in imaging the internal organs and structures with sufficient resolution and penetration depth. Even with established mutant lines that remain transparent throughout their life cycle, it is still challenging for purely optical imaging modalities to visualize the organs of juvenile and adult zebrafish at a micro-scale spatial resolution. In this work, we developed a non-invasive three-dimensional photoacoustic imaging platform with an optimized illumination pattern and a cylindrical-scanning-based data collection system to image entire zebrafish with micro-scale resolutions of 80 μm and 600 μm in the lateral and axial directions, respectively. In addition, we employed a multispectral strategy that utilized excitation wavelengths from 690 nm to 930 nm to statistically quantify the relative optical absorption spectrum of major organs.

Seed-Mediated Synthesis of Tunable-Aspect-Ratio Gold Nanorods for Near-Infrared Photoacoustic Imaging

“…Photoacoustic imaging is a non-invasive imaging modality offering an increased in vivo imaging depth and spatial resolution compared to other traditional optical imaging methods [ 37 – 40 ]. We found that AuNR-PEG with high NIR absorbance could be used as a great contrasting agent in photoacoustic imaging (Fig.…”

Three‐dimensional NMR microscopy of zebrafish specimens

“…Because of this pressing need, a gamut of modalities have been tried and used to image adult zebrafish: optical projection tomography (OPT), 1,2 photoacoustic tomography (PAT), 3,4 optical multispectral 3D tomography, 5 time‐gated optical projection tomography (TGOPT), 6 optical coherence tomography (OCT), 7 contrast‐enhanced X‐ray micro‐computed tomography, 8 synchrotron X‐ray micro‐computed tomography (SR‐CT), 9 ultrasound, 10 and NMR microscopy (MRM) ex vivo 11 and in vivo. 12–16…”

Assessing the development and treatment of rheumatoid arthritis using multiparametric photoacoustic and ultrasound imaging

“…The generated PA signals are collected by a 10 MHz cylindrically focused US transducer (V311, Olympus Inc.) and amplified at ~66 dB using a customized amplifier. The lateral and axial resolutions of the system were estimated to be 80 and 600 μm using phantom experiments, respectively . For US imaging, a pulse/receiver (5073PR, Olympus Inc.), synchronized with the output trigger of the OPO laser, drives a 7.5 MHz spherical‐focused transducer (V320, Olympus Inc.) to generate acoustic waves, records and amplifies the reflected acoustic signals.…”