Whole-Brain Map of Long-Range Monosynaptic Inputs to Different Cell Types in the Amygdala of the Mouse

Fu, Jiayu; Yu, Xiaodan; Zhu, Yi; Xie, Shi-Ze; Tang, Mengyu; Yu, Bin; Li, Xiaoming

doi:10.1007/s12264-020-00545-z

Cited by 39 publications

(26 citation statements)

References 64 publications

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“…However, due to methodological limitations, rabies-based tracing approaches might introduce some variations while quantifying, for example, brain-wide afferent input weights under different experimental conditions [ 17 ]. Indeed, the results from Fu et al [ 15 ], and those from our Vglut2-Cre and Thy1-Cre mice (Figs. 3 – 6 and S5 – 8 , Tables S1 – 4 ) share the similarity in input weight of general afferent stimulus categories, while differ somewhat in input weight of a few specific afferents.…”

Section: Discussionsupporting

confidence: 70%

“…The amygdala sends efferent signals to multiple brain areas to orchestrate defensive behaviors, and the main amygdalar output pathways seem to go through BNST, vHPC, mPFC and NAc to control initiation, acquisition, evaluation and decision-making of survival behaviors, respectively [ 1 , 6 ]. The previous studies have made significant effort to identify the sources of synaptic inputs to amygdalar neurons and/or the projection targets of these cells [ 1 , 4 , 15 , 16 ]. However, technical and/or design limitations preclude these studies from linking the afferent inputs with projection targets of specific amygdalar neuronal groups.…”

Section: Resultsmentioning

confidence: 99%

“…The new insights of BLA input-output organization shed light on how the amygdala converts input stimuli to output behavioral commands. Accumulating anatomical research, including the most recent studies, has consistently documented that the amygdala receives neural projections essentially from every brain area [ 1 , 4 , 15 , 16 ]. However, these studies stop short of defining the relationship of amygdalar input and output neuronal circuits.…”

Section: Discussionmentioning

confidence: 99%

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Organizational principles of amygdalar input-output neuronal circuits

et al. 2021

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The amygdala, one of the most studied brain structures, integrates brain-wide heterogeneous inputs and governs multidimensional outputs to control diverse behaviors central to survival, yet how amygdalar input-output neuronal circuits are organized remains unclear. Using a simplified cell-type- and projection-specific retrograde transsynaptic tracing technique, we scrutinized brain-wide afferent inputs of four major output neuronal groups in the amygdalar basolateral complex (BLA) that project to the bed nucleus of the stria terminals (BNST), ventral hippocampus (vHPC), medial prefrontal cortex (mPFC) and nucleus accumbens (NAc), respectively. Brain-wide input-output quantitative analysis unveils that BLA efferent neurons receive a diverse array of afferents with varied input weights and predominant contextual representation. Notably, the afferents received by BNST-, vHPC-, mPFC- and NAc-projecting BLA neurons exhibit virtually identical origins and input weights. These results indicate that the organization of amygdalar BLA input-output neuronal circuits follows the input-dependent and output-independent principles, ideal for integrating brain-wide diverse afferent stimuli to control parallel efferent actions. The data provide the objective basis for improving the virtual reality exposure therapy for anxiety disorders and validate the simplified cell-type- and projection-specific retrograde transsynaptic tracing method.

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Section: Discussionsupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Organizational principles of amygdalar input-output neuronal circuits

et al. 2021

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“…Immunostaining was then performed as previously described [ 32 , 33 ]. Mice were transcardially perfused with 4% paraformaldehyde (PFA), postfixed at 4 °C overnight, cryoprotected in 30% sucrose, and embedded in optimum cutting temperature (OCT) compound.…”

Section: Methodsmentioning

confidence: 99%

Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors

Liu

et al. 2021

Mol Brain

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The rodent whisker-barrel cortex system has been established as an ideal model for studying sensory information integration. The barrel cortex consists of barrel and septa columns that receive information input from the lemniscal and paralemniscal pathways, respectively. Layer 5a is involved in both barrel and septa circuits and play a key role in information integration. However, the role of layer 5a in the development of the barrel cortex remains unclear. Previously, we found that calretinin is dynamically expressed in layer 5a. In this study, we analyzed calretinin KO mice and found that the dendritic complexity and length of layer 5a pyramidal neurons were significantly decreased after calretinin ablation. The membrane excitability and excitatory synaptic transmission of layer 5a neurons were increased. Consequently, the organization of the barrels was impaired. Moreover, layer 4 spiny stellate cells were not able to properly gather, leading to abnormal formation of barrel walls as the ratio of barrel/septum size obviously decreased. Calretinin KO mice exhibited deficits in exploratory and whisker-associated tactile behaviors as well as social novelty preference. Our study expands our knowledge of layer 5a pyramidal neurons in the formation of barrel walls and deepens the understanding of the development of the whisker-barrel cortex system.

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“…Immunostaining was then performed as previously described [32,33]. Mice were transcardially perfused with 4% paraformaldehyde (PFA), post xed at 4°C overnight, cryoprotected in 30% sucrose, and embedded in optimum cutting temperature (OCT) compound.…”

Section: Immunostaining and Morphological Analysismentioning

confidence: 99%

Loss of Calretinin in L5a Impairs the Formation of the Barrel Cortex Leading to Abnormal Behaviors

Su¹,

Liu²,

Yu³

et al. 2021

Preprint

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The rodent whisker-barrel cortex system has been established as an ideal model for studying sensory information integration. The barrel cortex consists of barrel and septa columns that receive information input from the lemniscal and paralemniscal pathways, respectively. L5a is involved in both barrel and septa circuits and play a key role in information integration. However, the role of L5a in the development of the barrel cortex remains unclear. Previously, we found that Calretinin is dynamically expressed in L5a. In this study, we analyzed Cr KO mice and found that the dendritic complexity and length of L5a pyramidal neurons were significantly decreased after Cr ablation. The membrane excitability and excitatory synaptic transmission of L5a neurons were increased. Consequently, the organization of the barrels was impaired. Moreover, L4 spiny stellate cells were not able to properly gather, leading to abnormal formation of barrel walls as the ratio of barrel/septum size obviously decreased. Cr KO mice exhibited deficits in exploratory and whisker-associated tactile behaviors as well as social novelty preference. Our study expands our knowledge of L5a pyramidal neurons in the formation of barrel walls and deepens the understanding of the development of the whisker-barrel cortex system.

show abstract

Whole-Brain Map of Long-Range Monosynaptic Inputs to Different Cell Types in the Amygdala of the Mouse

Cited by 39 publications

References 64 publications

Organizational principles of amygdalar input-output neuronal circuits

Organizational principles of amygdalar input-output neuronal circuits

Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors

Loss of Calretinin in L5a Impairs the Formation of the Barrel Cortex Leading to Abnormal Behaviors

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