Whole cell bioconversion of (+)‐valencene to (+)‐nootkatone by <i>Yarrowia lipolytica</i> using a three phase partitioning bioreactor

Palmerín-Carreño, D.M.; Castillo‐Araiza, Carlos O.; Rutiaga-Quiñones, Olga Miriam; Calvo, J. R. Verde; Trejo-Aguilar, Gloria; Dutta, Abhishek; Huerta‐Ochoa, Sergio

doi:10.1002/jctb.4702

Cited by 25 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…52 After 149 h of fermentation, the final titer of total terpenes was 4816.12 mg/L, with (+)-nootkatone obtaining the highest titer of 2386.44 mg/L, 10-fold higher than that via shake-flask fermentation (214.09 mg/L). It was encouraging that this reached the highest de novo synthesis (+)-nootkatone yield to our knowledge, and it also well exceeded the yields previously reported in engineered S. cerevisiae strains or in other (+)-nootkatone producers, e.g., S. cerevisiae, 9 E. coli, 53,54 Yarrowia lipolytica, 55,56 and Komagataella phaffii (previously named Pichia pastoris) 10 (Table 1). Notably, throughout the 149 h fermentation process, the yield of (+)-nootkatone was always higher than that of (+)-valencene and β-nootkatol.…”

Section: De Novo Production Of (+)-Nootkatone From Glucose By Metabol...supporting

confidence: 77%

See 1 more Smart Citation

Systematic Optimization of HPO-CPR to Boost (+)-Nootkatone Synthesis in Engineered Saccharomyces cerevisiae

Liu

Chen

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

As an important and expensive natural sesquiterpene compound in grapefruit, the interest in (+)-nootkatone is stimulated by its strong grapefruit-like odor and physiological activities, which induce efforts for its microbial production. However, the low catalytic efficiency of the cytochrome P450-P450 reductase (HPO-CPR) system is the main challenge. We developed a highthroughput screening (HTS) method using the principle of the color reaction between carbonyl compounds and 2,4dinitrophenylhydrazine (DNPH), which could rapidly screen the activity of candidate HPO mutants. After optimizing the pairing of HPO and CPR and through semirational design, the optimal mutant HPO_M18 with catalytic performance 2.54 times that of the initial was obtained. An encouraging (+)-nootkatone titer of 2.39 g/L was achieved through two-stage fed-batch fermentation after metabolic engineering and endoplasmic reticulum engineering, representing the highest titer reported to date. Our findings lay the foundation for the development of an economically viable bioprocess for (+)-nootkatone.

show abstract

Section: De Novo Production Of (+)-Nootkatone From Glucose By Metabol...supporting

confidence: 77%

“…cerevisiae, E. coli, , Yarrowia lipolytica, , and Komagataella phaffii (previously named Pichia pastoris) (Table ). Notably, throughout the 149 h fermentation process, the yield of (+)-nootkatone was always higher than that of (+)-valencene and β-nootkatol.…”

Section: Resultsmentioning

confidence: 99%

Systematic Optimization of HPO-CPR to Boost (+)-Nootkatone Synthesis in Engineered Saccharomyces cerevisiae

Liu

Chen

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…At this time, the maximum nootkatone concentration was 628.41 ± 18.60 mg·L −1 and the conversion rate was 68.30 ± 2.02% at the conditions of 32°C, 150 rpm, and pH 5.30. It is slightly higher than that in a two‐phase system using 0.2% (w/v) of CTAB, 2.0 mmol·L −1 of niacin, and 22.5 g·L −1 of biomass (619.8 mg·L −1 ) (Palmerin‐Carreno, et al., 2016).…”

Section: Resultsmentioning

confidence: 92%

“…The research on the transformation of nootkatone by Y. lipolytica was shown in the Table 1. Palmerín‐Carreno et al studied about microorganisms for bioconversion of (+)‐valencene to (+)‐nootkatone in different phase system (Table 1) (Palmerin‐Carreno, et al., 2016; Palmerín‐Carreño et al., 2015; Palmerin‐Carreno et al., 2016). Guo et al constructed synthetic pathway of (+)‐ nootkatone in Y. lipolytica by heterologous expression of CnVS and CYP706M1 (Table 1) (Guo et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

Catalytic condition optimization in the conversion of nootkatone from valencene byYarrowia lipolytica

Xiao

Ren

Fan

et al. 2021

J. Food Process. Preserv.

View full text Add to dashboard Cite

Yarrowia lipolytica was a widespread and promising object in biotechnology, which was used to increase the biotransformation of valencene into nootkatone in shake flasks in this study. The main objective of this work is to determine the optimum combination of transformation conditions in shake flasks on the basis of single factor process optimization experiment. The results showed that Y. lipolytica was cultured in medium for 36 hr (exponential phase) and the amount of added Y. lipolytica was 5% (v/v). The concentration of substrate valencene was 920 mg·L−1, and the transformation time was 36 hr after adding substrates. Dimethyl sulfoxide, a water‐soluble organic solvent, was used as a co‐solvent with a concentration of 0.8% (v/v). The maximum production of nootkatone was 628.41 ± 18.60 mg·L−1 and the conversion rate was 68.30 ± 2.02% at cultivation conditions of 32°C, 150 rpm, and PH 5.30. The results showed that optimizing the catalytic conditions can significantly increase the yield of nootkatone by Y. lipolytica. Practical applications Recently, natural nootkatone is increasingly important due to its flavoring and physiological effects, but the amount of nootkatone in plants is low and cannot meet the demand. Also, there were few studies about microbial transformation of valencene by Yarrowia lipolytica under certain catalytic conditions. We demonstrated that optimizing the catalytic conditions can significantly increase the yield of nootkatone. Therefore, it provides a basis for the subsequent use of genetic engineering to transform Y. lipolytica to produce nootkatone.

show abstract

“…Botryosphaeria dothidea , Botryodiplodia theobromae , Phanerochaete chrysosporium , Mucor sp., and Chaetomium globosum could convert (+)-valencene into (+)-nootkatone. − In addition, the yeast Komagataella phaffii can be used as a whole-cell biocatalyst for the formation of (+)-nootkatone from (+)-valencene . Other reports of (+)-nootkatone production in Yarrowia lipolytica 2.2ab showed that the yield was up to 852.3 mg L –1 . Additionally, the edible fungus Pleurotus sapidus was reported to oxidize (+)-valencene to (+)-nootkatone. , …”

Section: Production Of Valencene and Nootkatonementioning

confidence: 99%

Production, Function, and Applications of the Sesquiterpenes Valencene and Nootkatone: a Comprehensive Review

Zhang

Chen

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Valencene and nootkatone, two sesquiterpenes, extracted from natural sources, have great market potential with diverse applications. This paper aims to comprehensively review the recent advances in valencene and nootkatone, including source, production, physicochemical and biological properties, safety and pharmacokinetics evaluation, potential uses, and their industrial applications as well as future research directions. Microbial biosynthesis offers a promising alternative approach for sustainable production of valencene and nootkatone. Both compounds exert various beneficial activities, including antimicrobial, insecticidal, antioxidant, anti-inflammatory, anticancer, cardioprotective, neuroprotective, hepatoprotective, and nephroprotective and other activities. However, most of the studies are performed in animals and in vitro, making it difficult to give a conclusive description about their health benefits and extend their application. Hence, more attention should be paid to in vivo and long-term clinical studies in the future. Moreover, valencene and nootkatone are considered safe for consumption and show great promise in the applications of food, cosmetic, pharmaceutical, chemical, and agricultural industries.

show abstract

Whole cell bioconversion of (+)‐valencene to (+)‐nootkatone by Yarrowia lipolytica using a three phase partitioning bioreactor

Cited by 25 publications

References 48 publications

Systematic Optimization of HPO-CPR to Boost (+)-Nootkatone Synthesis in Engineered Saccharomyces cerevisiae

Systematic Optimization of HPO-CPR to Boost (+)-Nootkatone Synthesis in Engineered Saccharomyces cerevisiae

Catalytic condition optimization in the conversion of nootkatone from valencene byYarrowia lipolytica

Production, Function, and Applications of the Sesquiterpenes Valencene and Nootkatone: a Comprehensive Review

Contact Info

Product

Resources

About