Whole Cell Biosensors

Bousse, L.

doi:10.1109/sensor.1995.721853

Cited by 44 publications

(43 citation statements)

References 41 publications

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“…Compact and portable hybrid devices incorporating whole-cell sensors directly mounted onto integrated circuits enabled bioluminescent quantification outside of the laboratory (9,14). Other examples include devices that mimicked natural biofilms by using protective cellular encapsulation in polymers, or whole-cell biosensing arrays for multiplexing (9,15,16). The trend of hybrid devices has mostly been abandoned for the convenience of plate readers because data can be collected using this platform in an automated and high-throughput fashion.…”

Section: Whole-cell Biosensingmentioning

confidence: 99%

Synthetic biology devices for in vitro and in vivo diagnostics

Slomovic

Pardee

Collins

2015

Proc. Natl. Acad. Sci. U.S.A.

303

228

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There is a growing need to enhance our capabilities in medical and environmental diagnostics. Synthetic biologists have begun to focus their biomolecular engineering approaches toward this goal, offering promising results that could lead to the development of new classes of inexpensive, rapidly deployable diagnostics. Many conventional diagnostics rely on antibody-based platforms that, although exquisitely sensitive, are slow and costly to generate and cannot readily confront rapidly emerging pathogens or be applied to orphan diseases. Synthetic biology, with its rational and short design-to-production cycles, has the potential to overcome many of these limitations. Synthetic biology devices, such as engineered gene circuits, bring new capabilities to molecular diagnostics, expanding the molecular detection palette, creating dynamic sensors, and untethering reactions from laboratory equipment. The field is also beginning to move toward in vivo diagnostics, which could provide near real-time surveillance of multiple pathological conditions. Here, we describe current efforts in synthetic biology, focusing on the translation of promising technologies into pragmatic diagnostic tools and platforms.synthetic biology | diagnostics | biosensing | synthetic gene networks | nanobiotechnology

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Section: Whole-cell Biosensingmentioning

confidence: 99%

Synthetic biology devices for in vitro and in vivo diagnostics

Slomovic

Pardee

Collins

2015

Proc. Natl. Acad. Sci. U.S.A.

303

228

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show abstract

“…Using whole cells as biological recognition elements in bioelectronic noses provides the opportunity to elicit full functional information since it necessarily involves the influence of www.intechopen.com the cellular signaling cascade on sensor response. Whole cell systems have, thus, possible applications in the fields of pharmacology, cell biology, toxicology and environmental measurements (Keusgen, 2002;Bousse, 1996).…”

Section: Bioelectronic Noses Based On Whole Cells Expressing Olfactormentioning

confidence: 99%

Bioelectronic Noses Based on Olfactory Receptors

Vidić¹

2010

Intelligent and Biosensors

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“…They can incorporate the plant or animal isolated cells or subcellular structures as well as the microbial cells. The most of the cell based biosensors developed to date fall into the subclass of microbial biosensors (D'Souza, 2001b;Bousse, 1996). This is due to the simplicity of the microorganisms' cultivation, the rich analytical prospects of the microbial cells (Racek, 1995) and their reliability when using as the base of the immobilized biocatalysts (Cassidy et al, 1996).…”

Section: Biosensor Designmentioning

confidence: 99%

“…The analysis of application of whole cells in biosensors can be found in reviews (Bousse, 1996;Ziegler, 2000;Bentley et al, 2001). Review (Murphy, 2006) presents the analysis of advantages of biosensors (DNA-, immunosensors, enzyme sensors with direct charge transfer, etc.)…”

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confidence: 99%