Whole-cell cascade for the preparation of enantiopure β-O-4 aryl ether compounds with glutathione recycling

Laccases in the Context of Potentially Cooperating Enzymes

“…In some cases, the basic functioning of related enzyme cooperations has already convincingly been demonstrated at lab scale. However, the successful implementation of such novel biocatalytic systems into practical applications mostly still necessitates to overcome issues related to, e.g., operational stability under real process conditions, product yield and purity, and economic as well as legal constraints (Ba et al 2018;Ba and Vinoth Kumar 2017;Husarcikova and Schallmey 2019;Picart et al 2017;Roth and Spiess 2015;Schlosser and Hofer 2002). Other potential cooperations between laccases and further enzymes remain hypothetical yet and still have to be proven and further explored with regard to their usability for practical applications.…”

“…Possible practical applications of intracellular β-etherases necessitate the regeneration of the essential cofactors GSH and NAD + (Husarcikova and Schallmey 2019;Husarcikova et al 2018;Roth and Spiess 2015). Picart et al (2017) used a laccaseredox mediator system (LMS) instead of a Cα-acting alcohol dehydrogenase in order to produce Cα carbonyl groups required for β-etherase activity, finally resulting in the successful depolymerization of natural lignin upon subsequent treatment with βetherases and a glutathione lyase.…”