Whole-cell MALDI-TOF: Rapid screening method in environmental microbiology

Microbial-Enhanced Heavy Oil Recovery under Laboratory Conditions by Bacillus firmus BG4 and Bacillus halodurans BG5 Isolated from Heavy Oil Fields

“…There are reports for the ability of native bacteria to mineralize crude oil hydrocarbons in oil contaminated sites [47,48]. Identification of the isolates were done using protein profiling by Bruker's MALDI Biotyper [36] and by 16S rRNA gene analysis showing >97% sequence identity with respective genes in the National Center for Biotechnology Information (NCBI) database [49].…”

“…Among the 40 isolates studied, the ones which showed maximum growth on agar plates were identified using a MALDI Biotyper (Bruker Daltonik GmbH, Bremen, Germany) [36] and 16S rDNA sequencing. For the Biotyper identification, a direct smearing method was used where 24 h-grown pure cultures were smeared on the target plate and layered with 1 µL sinapinic acid.…”

Plant-Microbe Partnerships for Enhanced Biodegradation of Polychlorinated Biphenyls

“…In a recent variation of the technique, whole-cell instead of extracted proteins or other molecules from environmental samples is used. Koubek et al ( 2012 ) in an interesting study compared three different methods, viz., NEFERM test 24, MALDI-TOF MS, and sequencing of 16S rRNA for taxonomic identifi cation of bacterial isolates recovered from sediments contaminated with PCB. Authors found that each method has its own limitation and advantage for resolution.…”

“…Authors found that each method has its own limitation and advantage for resolution. However, MALDI-TOF MS appeared as the most reliable method with immense potential for quick screening of clinical and environmental samples (Koubek et al 2012 ). Restriction patterns of PCR-amplifi ed fragments of particular gene can also be analyzed with MALDI-TOF MS (Taranenko et al 2002 ).…”

Plant–microorganism interactions in bioremediation of polychlorinated biphenyl-contaminated soil

“…This step was repeated two times. Eventually, samples were applied on MALDI target, drizzled with 1 ml of the saturated solution of a-cyano-4-hydroxycinnamic acid (SigmaAldrich, USA) in organic solution (50% acetonitrile, 2.5% trifluoroacetic acid), and directly screened as described earlier [19]. Flex Control software (Bruker Daltonics, Bremen, Germany) was used to record spectra in a linear positive mode at an accelerated voltage of 19 kV in the range from 2 to 20 kDa.…”