Whole-genome analyses of human adenovirus type 55 emerged in Tibet, Sichuan and Yunnan in China, in 2016

Liu, Yuan; Zhou, Yifan; Gu, Li H.; Zhang, Lin; Zhang, Xuelian; Chen, Maomao; Zou, Ziying; Qiu, Wei; Hu, Xiaobing; Fan, Quanshui

doi:10.1371/journal.pone.0189625

Cited by 19 publications

(24 citation statements)

References 18 publications

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“…In China, an increasing number of outbreaks of HAdV-B55 have been reported since March 2006 [25]. Epidemic outbreaks of HAdV-B55 have occurred in military camps, schools, and even hospitals in Hebei Province (February 2012) [38], Tianjin City (January 2013) [27], Beijing (2015; this study), Guangzhou City (2016) [28], Tibet, Sichuan and Yunnan Provinces (2016) [39].…”

Section: Discussionmentioning

confidence: 75%

An outbreak of acute respiratory infection at a training base in Beijing, China due to human adenovirus type B55

Peng

et al. 2020

BMC Infect Dis

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Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were coinfected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55.

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Section: Discussionmentioning

confidence: 75%

An outbreak of acute respiratory infection at a training base in Beijing, China due to human adenovirus type B55

Peng

et al. 2020

BMC Infect Dis

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“…Outbreaks of HAdV-associated ARI (HAdV-ARI) in adults have been reported in communal living populations, including school, hospital, and military populations [3][4][5][6][7][8]. HAdV-55, a novel recombinant strain of HAdV-11 and HAdV-14 that causes ARI in adults [9], emerged as an epidemic strain in Asian and European countries from 2004 [6,8,[10][11][12][13][14][15][16][17][18]. In the Republic of Korea, fatal cases of HAdV-55 pneumonia among military personnel have been reported since 2012 [19], and a large military outbreak associated with HAdV-55 was observed in the winter of 2014 [20].…”

Section: Introductionmentioning

confidence: 99%

Ongoing outbreak of human adenovirus-associated acute respiratory illness in the Republic of Korea military, 2013 to 2018

Woo

et al. 2021

Korean J Intern Med

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0000-0002-7464-9780 *These authors contributed equally to this work. Background/Aims: Human adenovirus type 55 (HAdV-55), an emerging epidemic strain, has caused several large outbreaks in the Korean military since 2014, and HAdV-associated acute respiratory illness (HAdV-ARI) has been continuously reported thereafter. Methods: To evaluate the epidemiologic characteristics of HAdV-ARI in the Korean military, we analyzed respiratory virus polymerase chain reaction (RV-PCR) results, pneumonia surveillance results, and severe HAdV cases from all 14 Korean military hospitals from January 2013 to May 2018 and compared these data with nationwide RV surveillance data for the civilian population. Results: A total of 14,630 RV-PCRs was performed at military hospitals. HAdV (45.4%) was the most frequently detected RV, followed by human rhinovirus (12.3%) and influenza virus (6.3%). The percentage of the military positive for HAdV was significantly greater than the percentage of civilians positive for HAdV throughout the study period, with a large outbreak occurring during the winter to spring of 2014 to 2015. The outbreak continued until the end of the study, and non-seasonal detections increased over time. The reported number of pneumonia patients also increased during the outbreak. Case fatality rate was 0.075% overall but 15.6% in patients with respiratory failure. The proportion of severe patients did not change significantly during the study period. Conclusions: A large HAdV outbreak is currently ongoing in the Korean military, with a trend away from seasonality, and HAdV-55 is likely the predominant strain. Persistent efforts to control the outbreak, HAdV type-specific surveillance, and vaccine development are required.

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“…Epidemic outbreaks of HAdV-B55 have occurred in military camps, schools, and even hospitals in Hebei Province (February 2012) [38], Tianjin City (January 2013) [27], Beijing (2015; this study), Guangzhou City (2016) [28], Tibet, and Sichuan and Yunnan Provinces (2016) [39].…”

Section: Discussionmentioning

confidence: 76%

An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

Peng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Twelve students experienced symptoms of acute respiratory infection (ARI) at a training base in Beijing from August 26 to August 30, 2015. We investigated the cause of this ARI outbreak. Methods: In partnership with the local center for disease control, we collected a total of twelve pharyngeal swab specimens as well as demographic information for the affected patients. We used multiplex real-time PCR to screen for sixteen common respiratory viruses in these samples. To isolate HAdV, we inoculated Hep-2 cells with the human adenovirus (HAdV)-positive samples and then carried out sequencing and phylogenetic analysis of the hexon, fiber, and penton genes of the isolated adenoviruses. In addition, we analyzed the entire genome of one strain isolated from the index case to identify single-nucleotide substitutions. Results: We identified ten HAdV-positive students using multiplex real-time PCR. None of the students were co-infected with other viruses. We successfully isolated seven HAdV strains from the pharyngeal swab specimens. The coding sequences of the hexon, fiber, and penton genes of these seven HAdV strains were identical, suggesting that they represented seven strains from a single virus clone. One HAdV isolate obtained from the index case, BJDX-01-2015, was selected for whole genome analysis. From this isolate, we obtained a 34,774-nucleotide sequence. The genome of BJDX-01-2015 clustered with HAdV-B55 in phylogenetic analyses and had 99.97% identity with human adenovirus 55 isolate HAdV-B/CHN/BJ01/2011/55 (GenBank accession no. JX491639). Conclusions: We identified HAdV-B55 as the strain associated with the August 2015 ARI outbreak at a training base in Beijing. This was the first reported outbreak in Beijing due to HAdV-B55. Continuous surveillance of respiratory adenoviruses is urgently needed to understand the epidemiological and evolutionary features of HAdV-B55, and an epidemiological modeling approach may provide further insights into this emerging public health threat. Furthermore, the clinical laboratory data from this outbreak provides important reference for the clinical diagnosis and may ultimately aid in informing the development of strategies to control and prevent respiratory tract infections caused by HAdV-B55.

show abstract

Whole-genome analyses of human adenovirus type 55 emerged in Tibet, Sichuan and Yunnan in China, in 2016

Cited by 19 publications

References 18 publications

An outbreak of acute respiratory infection at a training base in Beijing, China due to human adenovirus type B55

An outbreak of acute respiratory infection at a training base in Beijing, China due to human adenovirus type B55

Ongoing outbreak of human adenovirus-associated acute respiratory illness in the Republic of Korea military, 2013 to 2018

An Outbreak of Acute Respiratory Infection at a Training Base in Beijing, China Due to Human Adenovirus Type B55

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