Whole genome analysis for plant growth promotion profiling of Pantoea agglomerans CPHN2, a non-rhizobial nodule endophyte

Abstract

Reduced agricultural production as well as issues like nutrient-depleted soils, eutrophication, and groundwater contamination have drawn attention to the use of endophyte-based bioformulations to restore soil fertility. Pantoea agglomerans CPHN2, a non-rhizobial nodule endophyte isolated from Cicer arietinum, exhibited a variety of plant growth-promoting traits. In this study, we used NextSeq500 technology to analyze whole-genome sequence information of this plant growth-promoting endophytic bacteria. The genome of P. agglomerans CPHN2 has a length of 4,839,532 bp and a G + C content of 55.2%. The whole genome comprises three different genomic fractions, comprising one circular chromosome and two circular plasmids. A comparative analysis between P. agglomerans CPHN2 and 10 genetically similar strains was performed using a bacterial pan-genome pipeline. All the predicted and annotated gene sequences for plant growth promotions (PGPs), such as phosphate solubilization, siderophore synthesis, nitrogen metabolism, and indole-3-acetic acid (IAA) of P. agglomerans CPHN2, were identified. The whole-genome analysis of P. agglomerans CPHN2 provides an insight into the mechanisms underlying PGP by endophytes and its potential applications as a biofertilizer.

Phosphate Solubilization and Plant Growth Promotion by Pantoea brenneri Soil Isolates

“…Many microorganisms possess catabolic pathways to degrade these molecules [ 44 ]. Enzymes involved in phosphonate catabolic pathways are conserved and encoded by ortholog genes in bacteria [ 45 ]. P. brenneri possesses phn genes, which are responsible for phosphonate solubilization, making these bacteria universal phosphate solubilizer.…”

Complete genome sequence of Klebsiella variicola subsp. variicola ML.9ba2, an endophytic strain isolated from aerial roots of Philodendron erubescens

“…PGAP predicted numerous genes associated with plant-growth promotion and endophytism ( Table 1 ) ( 24 – 28 ) as well as a chromosomally encoded LEN-19 beta-lactamase (MKK01_RS13570) ( 29 ). Plasmid pML9ba2 was classified as type IncFIB(K)_5(cladeI) by the KpVR web-based tool ( https://bioinfo-mml.sjtu.edu.cn/KpVR/index.php ), which found no antimicrobial resistance genes.…”