2020
DOI: 10.3390/jcm9041055
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Whole-Genome DNA Methylation Profiling of CD14+ Monocytes Reveals Disease Status and Activity Differences in Crohn’s Disease Patients

Abstract: Crohn’s disease (CD) is a multifactorial incurable chronic disorder. Current medical treatment seeks to induce and maintain a state of remission. During episodes of inflammation, monocytes infiltrate the inflamed mucosa whereupon they differentiate into macrophages with a pro-inflammatory phenotype. Here, we sought to characterize the circulating monocytes by profiling their DNA methylome and relate it to the level of CD activity. We gathered an all-female age-matched cohort of 16 CD patients and 7 non-CD volu… Show more

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Cited by 17 publications
(17 citation statements)
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“…Crohn’s disease (CD) is a chronic disorder that belongs to the group of inflammatory bowel diseases (IBD); it is characterized by transmural inflammation that can affect any area along the proximal-distal axis of the gastrointestinal tract (GI) [ 1 , 2 , 3 ]. Symptoms often involve abdominal pain, diarrhea, rectal blood loss, and fatigue, and the disease often leads to weight loss and malnutrition [ 4 ].…”
Section: Introductionmentioning
confidence: 99%
“…Crohn’s disease (CD) is a chronic disorder that belongs to the group of inflammatory bowel diseases (IBD); it is characterized by transmural inflammation that can affect any area along the proximal-distal axis of the gastrointestinal tract (GI) [ 1 , 2 , 3 ]. Symptoms often involve abdominal pain, diarrhea, rectal blood loss, and fatigue, and the disease often leads to weight loss and malnutrition [ 4 ].…”
Section: Introductionmentioning
confidence: 99%
“…DNA methylation studies on blood samples from CD patients with inactive or active disease are diverse and included analyses on whole blood samples [ 54 ], peripheral blood cells [ 55 , 56 ], peripheral leukocytes [ 57 59 ], including peripheral blood mononuclear cells (PBMCs) [ 60 , 61 ], serum samples [ 62 ], or separated B cells [ 63 ], T cells [ 64 ], and monocytes [ 65 ] (Table 1 ). The majority of studies involve adult patients [ 54 56 , 58 , 60 , 62 , 63 , 65 ], while a few articles describe the DNA methylation in pediatric donors [ 57 , 59 , 61 , 64 ]. A comparison between these studies is difficult because DNA methylation is strongly affected by age, or environmental conditions, such as medication or nutrition [ 66 ].…”
Section: Epigenetic Investigation In Crohn's Diseasementioning
confidence: 99%
“…Thus, it is assumed that the epigenetic modifications more likely reflect the disease state. In addition to age, gender also influences methylation status, which is why some studies include only subjects of the same sex [ 54 , 58 , 65 ]. This leads to better comparability of results between patients and control subjects.…”
Section: Epigenetic Investigation In Crohn's Diseasementioning
confidence: 99%
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