Whole genome sequence analysis of Geitlerinema sp. FC II unveils competitive edge of the strain in marine cultivation system for biofuel production

Abstract

A filamentous cyanobacteria, Geitlerinema sp. FC II, was isolated from marine algae culture pond at Reliance Industries Limited (RIL), India. The 6.7 Mb draft genome of FC II encodes for 6697 protein coding genes. Analysis of the whole genome sequence revealed presence of nif gene cluster, supporting its capability to fix atmospheric nitrogen. FC II genome contains two variants of sulfide:quinone oxidoreductases (SQR), which is a crucial elector donor in cyanobacterial metabolic processes. FC II is characterized by the presence of multiple CRISPR- Cas (Clustered Regularly Interspaced Short Palindrome Repeats - CRISPR associated proteins) clusters, multiple variants of genes encoding photosystem reaction centres, biosynthetic gene clusters of alkane, polyketides and non-ribosomal peptides. Presence of these pathways will help FC II in gaining an ecological advantage over other strains for biomass production in large scale cultivation system. Hence, FC II may be used for production of biofuel and other industrially important metabolites.

Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects

“…Filamentous marine cyanobacteria such as Geitlerinema sp. FC II and Lyngbya confervoides Strain BDU141951 contain multiple CRISPR/Cas gene clusters [ 62 , 63 ]. The type III-B CRISPR/Cas systems exhibited a widespread distribution within Trichodesmium thiebautii while being absent in Trichodesmium erythraeum [ 64 ].…”

Stromatolites as Biosignatures of Atmospheric Oxygenation: Carbonate Biomineralization and UV-C Resilience in a Geitlerinema sp. - Dominated Culture

“…Many cyanobacteria including Geitlerinema sp. (Batchu et al, 2019), however, are able to simultaneously take up inorganic HCO 3 − , which is assimilated in the cytoplasm and converted into CO 2 in the carboxysome. Such CCMs are not only activated in hypersaline or hot environments, but especially in alkaline conditions when CO 3 2− dominates and allow the cells to survive CO 2 limitation (Kamennaya et al, 2012).…”