Whole-genome sequence data and analysis of type strains ‘Pusillimonas nitritireducens’ and ‘Pusillimonas subterraneus’ isolated from nitrate- and radionuclide-contaminated groundwater in Russia

Abstract

Two strains, ‘Pusillimonas nitritireducens’ JR1/69-2-13T and ‘Pusillimonas subterraneus’ JR1/69-3-13T, of aerobic, motile, Gram-negative, non-spore-forming, organotrophic, psychrotolerant bacteria were isolated from a sample of nitrate- and radionuclide-contaminated groundwater in Russia. Here we describe the draft genomes of these strains. The sequenced and annotated genome of the strain JR1/69-2-13T contained 4.3 Mbp with 4108 protein-coding genes. The genome of the strain JR1/69-3-13T contained 4.5 Mbp with 4260 protein-coding genes. Genome analysis of both strains provides an insight into the genomic basis of their resistance to nitrate, heavy metals and metalloids. The draft genome sequences of strains ‘Pusillimonas nitritireducens’ JR1/69-2-13T and ‘Pusillimonas subterraneus’ JR1/69-3-13T are available at DDBJ/EMBL/GenBank under the accession nos. https://www.ncbi.nlm.nih.gov/nuccore/PDNV00000000 and https://www.ncbi.nlm.nih.gov/nuccore/PDNW00000000, respectively.

Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater

“…Thus, analysis of genomes of the strains JR1/69-2-13 and JR1/69-3-13 testifies to their belonging to two novel species within the Pusillimonas genus with proposed names 'Pusillimonas nitritireducens' and 'Pusillimonas subterraneus', respectively [42]. The genome sequences of both strains provide an insight into the genomic basis of their resistance to heavy metals and metalloid and ability to survive in nitrate-contaminated groundwater.…”

“…Our previous study [42] demonstrated that average nucleotide identity (ANI) values of JR1/69-2-13 and JR1/69-3-13 genomes with genomes of Pusillimonas strains were within the range of 77.6%-84.5%. These ANI values were below the species border, proposed to be 95%-96% [51].…”

“…JR1/69-2-13, Pusillimonas sp. JR1/69-3-13 and Roseomonas aestuarii JR1/69-1-13 were sequenced and annotated as described previously [42,43]. Identification of protein-coding sequences and primary annotation was performed using the NCBI Prokaryotic Genome Automatic Annotation Pipeline (PGAAP) [44].…”

“…JR1/69-2-13 and Pusillimonas sp. JR1/69-3-13 were sequenced and annotated with the goal of elucidation of their taxonomic position and identification of the genetic determinants providing for their occurrence in the environment contaminated with radioactive waste [42].…”

Dynamics of Functional Genes and Bacterial Community during Bioremediation of Diesel-Contaminated Soil Amended with Compost

“…Halomonas has also been shown to contribute to the denitrifying process in an expanded granular sludge bioreactor(Miao et al 2018). Meanwhile, Pusillimonas was isolated from nitrate and radionuclide-contaminated groundwater, and shown to possess denitrifying functional genes(Grouzdev et al, 2018). Truepera has been reported as one of the denitri ers in a sequencing batch bio lm reactor used for land ll leachate treatment(Yin et al 2018), and exhibited a high dominance of over 20% in a similar denitri cation sequencing batch reactor(Shi et al, 2022).…”