Whole Genome Sequence of Alternaria alternata, the Causal Agent of Black Spot of Kiwifruit

Abstract

Alternaria alternata is a pathogen in a wide range of agriculture crops and causes significant economic losses. A strain of A. alternata (Y784-BC03) was isolated and identified from “Hongyang” kiwifruit and demonstrated to cause black spot infections on fruits. The genome sequence of Y784-BC03 was obtained using Nanopore MinION technology. The assembled genome is composed of 33,869,130bp (32.30Mb) comprising 10 chromosomes and 11,954 genes. A total of 2,180 virulence factors were predicted to be present in the obtained genome sequence. The virulence factors comprised genes encoding secondary metabolites, including non-host-specific toxins, cell wall-degrading enzymes, and major transcriptional regulators. The predicted gene clusters encoding genes for the biosynthesis and export of secondary metabolites in the genome of Y784-BC03 were associated with non-host-specific toxins, including cercosporin, dothistromin, and versicolorin B. Major transcriptional regulators of different mycotoxin biosynthesis pathways were identified, including the transcriptional regulators, polyketide synthase, P450 monooxygenase, and major facilitator superfamily transporters.

Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community

“…The yield and quality of crops are affected by many factors, among which the occurrence of diseases is usually the dominant one (Pan et al, 2021). Alternaria alternata is a facultative saprophytic fungus infecting over 400 species of host plants and ubiquitously causes leaf spot or leaf blight by synthesizing various kinds of mycotoxin, which pose threats to food safety and human health (Liu et al, 2020;Huang et al, 2021). Epidemiological studies also demonstrated that A. alternata released a large number of fungal spores in the atmosphere, making the disease hard to prevent and control (Woudenberg et al, 2015).…”

Genomic Based Analysis of the Biocontrol Species Trichoderma harzianum: A Model Resource of Structurally Diverse Pharmaceuticals and Biopesticides

“…While other Trichoderma sp. are known for the production of a few of the above predicted molecules, such as squalestatin, naphthopyrone, dimethylcoprogen, and clavaric acid [ 59 ], interestingly, our sequence analysis shed light on many more potential bioactive molecules that are most likely first reported in Trichoderma harzianum M10 v1.0.…”

Assembly of high‐quality genomes of the locoweed Oxytropis ochrocephala and its endophyte Alternaria oxytropis provides new evidence for their symbiotic relationship and swainsonine biosynthesis

“…Compared with seven other Alternaria species been sequenced, including A. gaisen , A. tenuissima , A. arborescens (Armitage et al, 2020), A. alternata (Huang et al, 2021), A. brassicicola (Belmas et al, 2018), A. solani (Wolters et al, 2018), A. longipes (Feng et al, 2022), A. panax (Qiu et al, 2022), A. brassicae and A. atra (Gebru et al, 2020), the genome size of A . oxytropis is above twice the size of those ones (70–112 Mb vs. 31–37 Mb, Table S33).…”