Whole-Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_15_17439_A144, Representative of a Human Outbreak in 2008

Orsini, Massimiliano; Ordinelli, Alessandra; Cornacchia, Alessandra; Acciari, Vicdalia Aniela; Centorame, Patrizia; Torresi, Marina; Pompei, Antonella; Marcacci, Maurilia; Ancora, Massimo; Domenico, Marco Di; Perticara, Samuel; Cammà, Cesare; Pasquale, Adriano Di; Gattuso, Antonietta; Gianfranceschi, Monica Virginia; Pomilio, Francesco

doi:10.1128/mra.00074-19

Cited by 4 publications

(7 citation statements)

References 11 publications

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“…The complete nucleotide (nt) sequences (50) and partial sequences (9) of plasmids representing each RFLP profile were obtained ( Figure 1 ). This pool includes complete sequences of five plasmids described in our previous studies: pLIS1 (GenBank: NZ_MH382833.1) [ 20 ], pLIS3 (NZ_MT912503.1) [ 24 ] and pLIS4-pLIS6 (NZ_MW124301.1; NZ_CP063072.1; NZ_MW124302.1) [ 23 ], as well as two plasmids that are identical to the previously sequenced L. monocytogenes plasmids pLmA144 (KU513859.1) (residing in a strain responsible for a human outbreak in Italy in 2008) and pMF4545 (CP025444.1) [ 25 , 26 ]. The sequences of 48 novel and unique replicons were obtained and named pLIS2, pLIS7-pLIS44, pLIS46-pLIS52 and pLIS54-pLIS55 (plasmid numbers do not refer to the RFLP groups) ( Figure 1 , Table S1 —including the affiliation of the individual plasmids to RFLP groups).…”

Section: Resultsmentioning

confidence: 99%

“…The sequences of 48 novel and unique replicons were obtained and named pLIS2, pLIS7-pLIS44, pLIS46-pLIS52 and pLIS54-pLIS55 (plasmid numbers do not refer to the RFLP groups) (Figure 1, Table S1-including the affiliation of the individual plasmids to RFLP groups). [25,26]. The sequences of 48 novel and unique replicons were obtained and named pLIS2, pLIS7-pLIS44, pLIS46-pLIS52 and pLIS54-pLIS55 (plasmid numbers do not refer to the RFLP groups) (Figure 1, Table S1-including the affiliation of the individual plasmids to RFLP groups).…”

Section: Identification and Classification Of Plasmids Occurring In 782 Listeria Spp Strainsmentioning

confidence: 99%

“…The following are available online at https://www.mdpi.com/article/ 10.3390/ijms221910320/s1. References [6,7,12,13,19,22,[24][25][26]28,40,41,57,73,[102][103][104][105][106][107][108][109][110][111][112][113][114] are cited in the supplementary materials…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…The following are available online at . References [ 6 , 7 , 12 , 13 , 19 , 22 , 24 , 25 , 26 , 28 , 40 , 41 , 57 , 73 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ] are cited in the supplementary materials.…”

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confidence: 99%

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Plasmidome of Listeria spp.—The repA-Family Business

Chmielowska

Korsak

Chapkauskaitse

et al. 2021

IJMS

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Bacteria of the genus Listeria (phylum Firmicutes) include both human and animal pathogens, as well as saprophytic strains. A common component of Listeria spp. genomes are plasmids, i.e., extrachromosomal replicons that contribute to gene flux in bacteria. This study provides an in-depth insight into the structure, diversity and evolution of plasmids occurring in Listeria strains inhabiting various environments under different anthropogenic pressures. Apart from the components of the conserved plasmid backbone (providing replication, stable maintenance and conjugational transfer functions), these replicons contain numerous adaptive genes possibly involved in: (i) resistance to antibiotics, heavy metals, metalloids and sanitizers, and (ii) responses to heat, oxidative, acid and high salinity stressors. Their genomes are also enriched by numerous transposable elements, which have influenced the plasmid architecture. The plasmidome of Listeria is dominated by a group of related replicons encoding the RepA replication initiation protein. Detailed comparative analyses provide valuable data on the level of conservation of these replicons and their role in shaping the structure of the Listeria pangenome, as well as their relationship to plasmids of other genera of Firmicutes, which demonstrates the range and direction of flow of genetic information in this important group of bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Identification and Classification Of Plasmids Occurring In 782 Listeria Spp Strainsmentioning

confidence: 99%

Section: Supplementary Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

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Plasmidome of Listeria spp.—The repA-Family Business

Chmielowska

Korsak

Chapkauskaitse

et al. 2021

IJMS

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“…The preliminary assembly of the entire genome showed 99.38% sequence identity, calculated by the OrthoANI v1.2 tool (6), to an L. monocytogenes 1/2a isolate (GenBank accession no. CP013919) identified by our group during an Italian foodborne outbreak investigation in 2008 (7) and 99.93% to 99.95% sequence identity, calculated by the OrthoANI v1.2 tool, to a group of isolates detected in Canada (i.e., CP007019, CP018685, CP001604, CP007008, CP007017, CP006861, CP007007, CP007011, CP009258, and CP008836) from clinical patients and food samples collected from 1998 to 2010. The closest reference genome was chosen by submitting raw contigs to the KmerFinder Web server (https://cge.cbs.dtu.dk/services/KmerFinder/).…”

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confidence: 99%

Draft Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_14-16064, Isolated from an Italian Cooked Ham in 2014

Lollo

Orsini

Acciari

et al. 2020

Microbiol Resour Announc

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Genomic Determinants of Pathogenicity and Antimicrobial Resistance for 60 Global Listeria monocytogenes Isolates Responsible for Invasive Infections

Shi

Anwar

Pan

et al. 2021

Front. Cell. Infect. Microbiol.

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Listeria monocytogenes remains a significant public health threat, causing invasive listeriosis manifested as septicemia, meningitis, and abortion, with up to 30% of cases having a fatal outcome. Tracking the spread of invasive listeriosis requires an updated knowledge for virulence factors (VFs) and antimicrobial resistance features, which is an essential step toward its clinical diagnosis and treatment. Taking advantage of high-throughput genomic sequencing, we proposed that the differential genes based on the pathogenomic composition could be used to evaluate clinical observations and therapeutic options for listeriosis. Here, we performed the comparative genomic analysis of 60 strains from five continents with a diverse range of sources, representing serotypes 1/2a, 1/2b, 1/2c, and 4b, comprising lineage I and lineage II and including 13 newly contributed Chinese isolates from clinical cases. These strains were associated with globally distributed clonal groups linked with confirmed foodborne listeriosis outbreak and sporadic cases. We found that L. monocytogenes strains from clonal complex (CC) CC8, CC7, CC9, and CC415 carried most of the adherence and invasive genes. Conversely, CC1, CC2, CC4, and CC6 have the least number of adherence and invasive genes. Additionally, Listeria pathogenicity island-1 (LIPI-1), LIPI-2, intracellular survival, surface anchoring, and bile salt resistance genes were detected in all isolates. Importantly, LIPI-3 genes were harbored in CC3, CC224, and ST619 of the Chinese isolates and in CC1, CC4, and CC6 of other worldwide isolates. Notably, Chinese isolates belonging to CC14 carried antibiotic resistance genes (ARGs) against β-lactams (blaTEM-101, blaTEM-105) and macrolide (ermC-15), whereas CC7 and CC8 isolates harbored ARGs against aminoglycoside (aadA10_2, aadA6_1), which may pose a threat to therapeutic efficacy. Phylogenomic analysis showed that CC8, CC7, and CC5 of Chinese isolates, CC8 (Swiss and Italian isolates), and CC5 and CC7 (Canadian isolates) are closely clustered together and belonged to the same CC. Additionally, CC381 and CC29 of Chinese isolates shared the same genomic pattern as CC26 of Swiss isolate and CC37 of Canadian isolate, respectively, indicating strong phylogenomic relation between these isolates. Collectively, this study highlights considerable clonal diversity with well-recognized virulence and antimicrobial-resistant determinants among Chinese and worldwide isolates that stress to design improved strategies for clinical therapies.

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Whole-Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_15_17439_A144, Representative of a Human Outbreak in 2008

Abstract: Here, we report the genome sequence of Listeria monocytogenes serovar 1/2a strain IZSAM_Lm_15_17439_A144, isolated in Italy from a patient during a Listeria monocytogenes outbreak in 2008. This strain showed 98.9% sequence identity to a strain isolated in Canada in the same year.

Cited by 4 publications

References 11 publications

Plasmidome of Listeria spp.—The repA-Family Business

Plasmidome of Listeria spp.—The repA-Family Business

Draft Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_14-16064, Isolated from an Italian Cooked Ham in 2014

Genomic Determinants of Pathogenicity and Antimicrobial Resistance for 60 Global Listeria monocytogenes Isolates Responsible for Invasive Infections

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