Whole genome sequence of Oscheius sp. TEL-2014 entomopathogenic nematodes isolated from South Africa

Lephoto, Tiisetso E.; Mpangase, Phelelani T.; Aron, Shaun; Gray, Vincent M.

doi:10.1016/j.gdata.2016.01.017

Cited by 8 publications

(6 citation statements)

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“…genomes, along with stage-specific transcriptomes [15] represents a valuable resource, alongside the previously published genomes of Oscheius sp. TEL-2014 [16] and Heterorhabditis bacteriophora [17]. The genome of Steinernema carpocapsae is the most complete, at an estimated 85.6 Mb, with predicted coverage of 98% [15].…”

Section: Introductionmentioning

confidence: 99%

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

et al. 2017

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Entomopathogenic nematodes (EPNs) employ a sophisticated chemosensory apparatus to detect potential hosts. Understanding the molecular basis of relevant host-finding behaviours could facilitate improved EPN biocontrol approaches, and could lend insight to similar behaviours in economically important mammalian parasites. FMRFamide-like peptides are enriched and conserved across the Phylum Nematoda, and have been linked with motor and sensory function, including dispersal and aggregating behaviours in the free living nematode Caenorhabditis elegans. The RNA interference (RNAi) pathway of Steinernema carpocapsae was characterised in silico, and employed to knockdown the expression of the FMRFamide-like peptide 21 (GLGPRPLRFamide) gene (flp-21) in S. carpocapsae infective juveniles; a first instance of RNAi in this genus, and a first in an infective juvenile of any EPN species. Our data show that 5 mg/ml dsRNA and 50 mM serotonin triggers statistically significant flp-21 knockdown (-84%***) over a 48 h timecourse, which inhibits host-finding (chemosensory), dispersal, hyperactive nictation and jumping behaviours. However, whilst 1 mg/ml dsRNA and 50 mM serotonin also triggers statistically significant flp-21 knockdown (-51%**) over a 48 h timecourse, it does not trigger the null sensory phenotypes; statistically significant target knockdown can still lead to false negative results, necessitating appropriate experimental design. SPME GC-MS volatile profiles of two EPN hosts, Galleria mellonella and Tenebrio molitor reveal an array of shared and unique compounds; these differences had no impact on null flp-21 RNAi phenotypes for the behaviours assayed. Localisation of flp-21 / FLP-21 to paired anterior neurons by whole mount in situ hybridisation and immunocytochemistry corroborates the RNAi data, further suggesting a role in sensory modulation. These data can underpin efforts to study these behaviours in other economically important parasites, and could facilitate molecular approaches to EPN strain improvement for biocontrol.

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Section: Introductionmentioning

confidence: 99%

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

et al. 2017

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“…genomes, along with stage-specific transcriptomes (15) represents a valuable resource, alongside the previously published genomes of Oscheius sp. TEL-2014 (16) and Heterorhabditis bacteriophora (17). The genome of Steinernema carpocapsae is the most complete, at an estimated 85.6 Mb, with predicted coverage of 98% (15).…”

Section: Introductionmentioning

confidence: 99%

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

Morris

Wilson

Warnock

et al. 2016

Preprint

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16Entomopathogenic nematodes (EPNs) employ a sophisticated chemosensory apparatus to 17 detect potential hosts. Understanding the molecular basis of relevant host-finding behaviours 18 could facilitate improved EPN biocontrol approaches, and could lend insight to similar 19 behaviours in economically important mammalian parasites. FMRFamide-like peptides are 20 enriched and conserved across the Phylum Nematoda, and have been linked with motor and 21 sensory function, including dispersal and aggregating behaviours in the free living nematode 22Caenorhabditis elegans. The RNA interference (RNAi) pathway of Steinernema 23 . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/061101 doi: bioRxiv preprint first posted online Jun. 28, 2016; carpocapsae was characterised in silico, and employed to knockdown the expression of the 24 FMRFamide-like protein 21 (GLGPRPLRFamide) gene (flp-21) in S. carpocapsae infective 25 juveniles; a first instance of RNAi in this genus, and a first in an infective juvenile of any EPN 26 species. Our data show that 5 mg/ml dsRNA and 50 mM serotonin triggers statistically 27 significant flp-21 knockdown (-84%***) over a 48 h timecourse, which inhibits host-finding 28 (chemosensory), dispersal, hyperactive nictation and jumping behaviours. However, whilst 1 29 mg/ml dsRNA and 50 mM serotonin also triggers statistically significant flp-21 knockdown (-30 51%**) over a 48 h timecourse, it does not trigger the null sensory phenotypes; statistically 31 significant target knockdown can still lead to false negative results, necessitating appropriate 32 experimental design. SPME GC-MS volatile profiles of two EPN hosts, Galleria mellonella 33and Tenebrio molitor reveal an array of shared and unique compounds; these differences 34 28, 2016; basis of behaviour. Here we have characterised the RNAi pathway of Steinernema 51 carpocapsae through analysing the genome sequence for relevant genes, and have 52 successfully knocked down the neuropeptide gene flp-21 in S. carpocapsae infective 53 juveniles. We find that it is involved in the regulation of behaviours which rely on sensory 54 perception and relate to host-finding. We have localised the gene and mature neuropeptide, 55 and find them to be expressed in paired anterior neurons, which is in broad agreement with 56 our behavioural observations following RNAi. Our observations are relevant to interactions 57 of S. carpocapsae with two insect hosts, the waxworm Galleria mellonella, and the 58 meelworm, Tenebrio molitor. We identified the volatile compounds relating to both insects, 59is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . http://dx.doi.org/10.1101/061101 doi: bioRxiv preprint first po...

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“…A Blast search of the entomopathogenic Rhabditidid Oscheius sp . (Lephoto et al 2016) against the G. legneri genome assembly revealed two hits, upon which we removed one contig and one partial contig from the assembly. A. bilineata contained Wolbachia , but no other co-sequenced genomes.…”

Section: Resultsmentioning

confidence: 99%

Genomic Resources forGoniozus legneri,Aleochara bilineataandPaykullia maculata, Representing Three Independent Origins of the Parasitoid Lifestyle in Insects

Kraaijeveld

Neleman

Mariën

et al. 2019

G3 Genes|Genomes|Genetics

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Parasitoid insects are important model systems for a multitude of biological research topics and widely used as biological control agents against insect pests. While the parasitoid lifestyle has evolved numerous times in different insect groups, research has focused almost exclusively on Hymenoptera from the Parasitica clade. The genomes of several members of this group have been sequenced, but no genomic resources are available from any of the other, independent evolutionary origins of the parasitoid lifestyle. Our aim here was to develop genomic resources for three parasitoid insects outside the Parasitica. We present draft genome assemblies for Goniozus legneri , a parasitoid Hymenopteran more closely related to the non-parasitoid wasps and bees than to the Parasitica wasps, the Coleopteran parasitoid Aleochara bilineata and the Dipteran parasitoid Paykullia maculata . The genome assemblies are fragmented, but complete in terms of gene content. We also provide preliminary structural annotations. We anticipate that these genomic resources will be valuable for testing the generality of findings obtained from Parasitica wasps in future comparative studies.

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Whole genome sequence of Oscheius sp. TEL-2014 entomopathogenic nematodes isolated from South Africa

Cited by 8 publications

References 12 publications

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

A neuropeptide modulates sensory perception in the entomopathogenic nematode Steinernema carpocapsae

Genomic Resources forGoniozus legneri,Aleochara bilineataandPaykullia maculata, Representing Three Independent Origins of the Parasitoid Lifestyle in Insects

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