Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values

Huo, Jinxi; Su, Zhong; Du, Xin; Cao, Yanwei; Wang, Wen-Qiong; Sun, Yan; Tian, Yu; Zhu, Jianxun; Chen, Jine; Liu, Xuan; Wu, Chongming; Li, Yougui

doi:10.1016/j.jare.2020.04.011

Cited by 31 publications

(31 citation statements)

References 65 publications

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“…Accumulating evidence acknowledged that SH extracts possessed medical usage against in ammation or cancer; for example, the whole-genome sequencing of Phellinus gilvus (mulberry Sanghuang) reveals the unique medicinal values [15], SH impacted growth, immunity, and fecal microbiota in pigs [18].…”

Section: Discussionmentioning

confidence: 99%

“…After centrifugation, the supernatant was collected and concentrated using rotary evaporation and lyophilized in a freeze vacuum and then collected, weighed, and stored at -20 o C until use. Our procedure was bale to make one kilogram of P. gilvus fruiting body to approximately 125.7 grams of SH, while the polyphenol content could yield 47.77% as determined by a colorimetric method 15 .…”

Section: Sh Preparationmentioning

confidence: 99%

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Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Li¹,

Sun²,

Su³

et al. 2020

Preprint

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Background: The gut microbiota plays crucial roles in various diseases and mediates the therapeutic effect of many drugs. Results: In this study, we found that ingestion of Phellinus gilvus extract (SH), a traditional Chinese medical mushroom, effectively ameliorated dextran sodium sulfate (DSS)-induced colits in mice, and the gut microbes were essential for its therapeutic effect. The enrichment of Alistipes spp. especially A. onderdonkii was highly associated with the amelioration of colitis by SH, and oral gavage of live A. onderdonkii potently and strain-specifically mitigated DSS-induced colitis in mice. 5-Hydroxyindole-3-acetic acid (5HIAA), a structural analogue of a known aromatic hydrocarbon receptor (AhR) activator indole-3-acetic acid (IAA), was significantly enriched after SH treatment and exhibited more potent anti-colitis efficiency than IAA in mice.Conclusion: Our results demonstrated the gut microbiota-derived 5HIAA could be an effective candidate in treatment of colitis, and modulation of the gut microbiota-indole derives-AhR pathway may be a novel strategy in development of next-generational anti-colitis drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Sh Preparationmentioning

confidence: 99%

Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Li¹,

Sun²,

Su³

et al. 2020

Preprint

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“…The total content of polyphenols was measured using Folin‐Ciocalteu method (Ma et al., 2019). The major compounds were identified by high‐performance liquid chromatography (HPLC) and high resolution electrospray ionization mass spectroscopy (HR‐ESI‐MS) method (Huo et al., 2020). The aqueous extracts treated with three volumes of ethanol, the supernatant was collected after centrifugation for HPLC and HR‐ESI‐MS analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Sanghuang have been used in Chinese traditional medicine for over 2000 years to treat various diseases. In recent years, modern pharmacological researches display that Sanghuang have multifunction such as anti‐carcinogenesis, anti‐inflammatory, anti‐oxidative, anti‐fungal, and immunomodulatory activities (Cai et al., 2019; Chen et al., 2019; Huo et al., 2020; Zhu & Cui, 2016). These active effects provided great potential for their application in medicinal and food industries.…”

Section: Introductionmentioning

confidence: 99%

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Safety evaluation of aqueous extracts of Sanghuangporus vaninii fruiting body in Sprague–Dawley rats

Huo

Sun

et al. 2020

Food Science & Nutrition

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Sanghuangporus vaninii, called “Sanghuang,” is orally used for health care, tumor, and inflammation treatment in Asia. However, the safety of S. vaninii has not been evaluated. The major compounds analysis showed that aqueous extracts of S. vaninii fruiting body were rich in polysaccharides, nucleotides, and polyphenols. Then, the aqueous was given orally to Sprague–Dawley rats for toxical test. In acute toxicity study, the maximum tolerated dose was 21 g/kg. In 17‐week repeated dose toxicity experiment, all rats had no abnormal reaction among control group, low dose group (0.15 g/kg) and middle dose group (1.00 g/kg). At high dose group (6.00 g/kg), the feces began to darken on 16th day (D16), and turned to drug stained stool on D21, all rats recovered on the 3rd day (D92) of recovery period. During the whole experiment, there were no animal death and no treatment‐related changes in any of the parameters under the all doses. These results indicated the No‐Observed Adverse Effect Level of aqueous extract of S. vaninii fruiting body was 1.0 g/kg.

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Development of RP HPLC‐PDA method for simultaneous quantitative analysis of Inoscavin A and Meshimakobnol A and application on some Phellinus mushroom species

Le,

Nguyen,

Dong

2024

Food Science & Nutrition

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Phellinus igniarius, a medicinal mushroom containing many active ingredients with health benefits, can be applied in functional food. At present, the quantification of the main active ingredients from higher fungi (Ganoderma, Phellinus…) materials from different growing sources is a mandatory requirement to standardize the input resources of pharmaceutical and food production. Our study's aims are to perfect the RP HPLC‐PDA method for quantitative analysis of Inoscavin A and Meshimakobnol A which are two main active ingredients present in Phellinus mushroom. In this analytical method, a C18‐HPLC column and the mixture of methanol and formic acid solutions (pH = 2.2) are used to analyze and elute the active substances with the column activity parameters being the concentration gradient. This perfect method was tested for system suitability, repeatability, intermediate precision, recovery, and linear curve calibration to validate the method. After validation, the perfected RP HPLC‐PDA method was applied to analyze eight samples of Phellinus and three samples of Ganoderma mushroom category. This method can be the basis for classifying between Phellinus and some other medicinal mushrooms.

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Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values

Cited by 31 publications

References 65 publications

Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Gut microbiota-derived 5-hydroxyindole-3-acetic acid mediates the anti-colitis effect of Phellinus gilvus in mice

Safety evaluation of aqueous extracts of Sanghuangporus vaninii fruiting body in Sprague–Dawley rats

Development of RP HPLC‐PDA method for simultaneous quantitative analysis of Inoscavin A and Meshimakobnol A and application on some Phellinus mushroom species

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