Whole-Genome Sequence of Pseudomonas xanthomarina Strain UASWS0955, a Potential Biological Agent for Agricultural and Environmental Uses

Crovadore, Julien; Cochard, Bastien; Calmin, Gautier; Chablais, Romain; Schulz, Torsten; Lefort, François

doi:10.1128/genomea.01136-16

Cited by 6 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CR isolates from seawater and stormwater samples often belonged to the same species (see Fig. S3 and Table S2) and included Pseudomonas asplenii, P. fulva, P. monteilii, P. taiwanensis, P. xanthomarina, Microbacterium liquefaciens, and Stenotrophomonas maltophilia, which are typically found in soil, freshwater, and sewage (53)(54)(55)(56). The stormwater outfalls in our local setting discharged directly into the ocean and hence clearly have a major influence on the CRB diversity in the coastal seawater, similar to what has been found in other coastal settings (57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

Diversity and Genetic Basis for Carbapenem Resistance in a Coastal Marine Environment

Dewi

Götz

Thomas

2020

Appl Environ Microbiol

View full text Add to dashboard Cite

Resistance to the “last-resort” antibiotics, such as carbapenems, has led to very few antibiotics being left to treat infections by multidrug-resistant bacteria. Spread of carbapenem resistance (CR) has been well characterized for the clinical environment. However, there is a lack of information about its environmental distribution. Our study reveals that CR is present in a wide range of Gram-negative bacteria in the coastal seawater environment, including four phyla, eight classes, and 30 genera. These bacteria were likely introduced into seawater via stormwater flows. Some CR isolates found here, such as Acinetobacter junii, Acinetobacter johnsonii, Brevundimonas vesicularis, Enterococcus durans, Pseudomonas monteilii, Pseudomonas fulva, and Stenotrophomonas maltophilia, are further relevant to human health. We also describe a novel metallo-β-lactamase (MBL) for marine Rheinheimera isolates with CR, which has likely been horizontally transferred to Citrobacter freundii or Enterobacter cloacae. In contrast, another MBL of the New Delhi type was likely acquired by environmental Variovorax isolates from Escherichia coli, Klebsiella pneumoniae, or Acinetobacter baumannii utilizing a plasmid. Our findings add to the growing body of evidence that the aquatic environment is both a reservoir and a vector for novel CR genes. IMPORTANCE Resistance against the “last-resort” antibiotics of the carbapenem family is often based on the production of carbapenemases, and this has been frequently observed in clinical samples. However, the dissemination of carbapenem resistance (CR) in the environment has been less well explored. Our study shows that CR is commonly found in a range of bacterial taxa in the coastal aquatic environment and can involve the exchange of novel metallo-β-lactamases from typical environmental bacteria to potential human pathogens or vice versa. The outcomes of this study contribute to a better understanding of how aquatic and marine bacteria can act as reservoirs and vectors for CR outside the clinical setting.

show abstract

Section: Discussionmentioning

confidence: 99%

Diversity and Genetic Basis for Carbapenem Resistance in a Coastal Marine Environment

Dewi

Götz

Thomas

2020

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In silico methods can be used to characterise various phytases, 3 for phytogenetic analysis, 4 to determine further necessary genetic manipulations when developing new phytases 5,6 and to predict the stability of these enzymes. 7,8 However, such studies are not able to predict phytase efficacy, since gene expression will not always correlate with phytase activity.…”

Section: Introductionmentioning

confidence: 99%

In vitro assays for evaluating phytate degradation in non‐ruminants: chances and limitations

Sommerfeld

Santos

2021

J Sci Food Agric

View full text Add to dashboard Cite

The positive effects of phytases on the environment, animal welfare and animal feed costs have resulted in the continuous development and improvement of these enzymes in the non-ruminant feed market. To test the efficacy of these phytases, a large number of experimental animals are necessary, antagonising the animal welfare aspect of these enzymes. In the present review, we summarise the most prominent available in vitro assays for evaluating phytase enzymes and how far they can reduce the number of in vivo experiments. Several in vitro assays exist that differ in their setup, extent and conditions depending on the animal of interest and the research question. With the in vitro assays described, it is not possible to fully replace in vivo trials. However, for the investigation of phytase effects in feedstuffs, the use of an in vitro assay has several advantages. In vitro assays have the potential to be used for ranking feed enzymes and as screening tools. Applying in vitro protocols will result in a reduction in the number of animals or treatments usually necessary for an in vivo trial, thus acting towards the three Rs.

show abstract

“…These species all had a higher relative abundance in the wet season, which was consistent with that of their genera. Among these species, P. xanthomarina has been considered a promising resource for agriculture and bioremediation, which could effectively degrade numerous hazardous substances such as polycyclic aromatic hydrocarbons (PAHs) and malathion [55][56][57]. These researches indicated that P. xanthomarina has the potential as a probiotic in aquaculture, but further research is needed to draw firm conclusions.…”

Section: Comparison Of Gut Bacterial Communities Among Different Seasonsmentioning

confidence: 99%

Comparative Analysis of Gut Bacterial Community Composition in Two Tropical Economic Sea Cucumbers under Different Seasons of Artificial Environment

Jia,

Wang,

Zheng

et al. 2024

IJMS

View full text Add to dashboard Cite

With the continuous rise of the sea cucumber aquaculture industry in China, the tropical sea cucumber aquaculture industry is also improving. However, research on the gut microorganisms of tropical sea cucumbers in captivity is scarce. In this study, high-throughput sequencing methods were used to analyze the gut microbial composition of Stichopus monotuberculatus and Holothuria scabra in the dry season and wet season of artificial environments. The results showed that 66 phyla were obtained in all samples, of which 59 phyla were obtained in the dry season, and 45 phyla were obtained in the wet season. The Tax4Fun analysis showed that certain gut bacterial communities affect the daily metabolism of two sea cucumber species and are involved in maintaining gut microecological balance in the gut of two sea cucumber species. In addition, compared with differences between species, PCoA and UPGMA clustering analysis showed the gut prokaryotes of the same sea cucumber species varied more in different seasons, indicating that the influence of environment was higher than the feeding choices of sea cucumbers under relatively closed conditions. These results revealed the gut bacterial community composition of S. monotuberculatus and H. scabra and the differences in gut bacterial structure between two sea cucumber species in different seasons were compared, which would provide the foundation for tropical sea cucumber aquaculture in the future.

show abstract

Whole-Genome Sequence of Pseudomonas xanthomarina Strain UASWS0955, a Potential Biological Agent for Agricultural and Environmental Uses

Cited by 6 publications

References 19 publications

Diversity and Genetic Basis for Carbapenem Resistance in a Coastal Marine Environment

Diversity and Genetic Basis for Carbapenem Resistance in a Coastal Marine Environment

In vitro assays for evaluating phytate degradation in non‐ruminants: chances and limitations

Comparative Analysis of Gut Bacterial Community Composition in Two Tropical Economic Sea Cucumbers under Different Seasons of Artificial Environment

Contact Info

Product

Resources

About