Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm

Milanez, Guilherme Paier; Nascimento, Leandro Costa do; Tirabassi, Adriane Holtz; Zuanaze, Marcelo; Rodrigues, Dália P.; Pereira, Gonçalo Amarante Guimarães; Brocchi, Marcelo

doi:10.1128/genomea.00340-16

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…Salmonella Enteritidis is known to cause human diseases with pandemic proportions (39).Salmonella Enteritidis ST11 was identified as a stable lineage and highly clonal ST isolated from humans (n=27), bovines (n=1), liquid eggs (n=1), and eggshells (n=1) in Japan (40) and from clinical samples, beef, and pork in Korea (41). It is the most prevalent ST (44/46) isolated from clinical samples and poultry in Brazil (42,43) and the only ST common to human, poultry, and seabirds in Chile (44). In addition, ST11 was also reported in chicken meat (n=76) in Iran (45) and beef meat in Morocco (46) and it is the common ST harboring in the genome extended-spectrum β-lactamases (ESBL) such as CTX-M-14 and CTX-M15 that have been isolated from stool and blood samples of people in Korea (47).…”

Section: Discussionmentioning

confidence: 99%

A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia

Fandiño

Verján

2019

biomedica

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Introducción. Salmonella Enteritidis es una de las mayores causas de salmonelosis en el mundo, siendo los huevos contaminados y la carne de pollo cruda sus principales fuentes de infección. En Ibagué, Colombia, se identificaron los principales serovares circulando en granjas, superficies de huevos y canales de pollo, sin embargo, se desconoce si esos serovares son responsables de gastroenteritis. Objetivo. Evaluar la relación genética entre aislamientos de Salmonella Enteritidis de aves de corral y humanos con gastroenteritis mediante multilocus sequence typing (MLST). Materiales y métodos. Se aisló Salmonella spp., de casos clínicos de gastroenteritis (n=110). Se realizó test de sensibilidad antibiótica, seguido de serotipificación y tipificación por medio de MLST y se comparó S. Enteritidis de humanos frente a S. Enteritidis de granjas ponedoras y de huevo comercializado (n=6). Resultados. Se aislaron 10 cepas de Salmonella spp., a partir de heces de humanos con gastroenteritis. Se obtuvo una prevalencia de Salmonella spp. de 9.09%, siendo S. Enteritidis (n=4), S. Typhymurium (n=2), S. Newport (n=1), S. Grupensis (n=1), S. Uganda (n=1) y S. Braenderup (n=1) los serotipos presentes en pacientes con gastroenteritis. El MLST indico que un tipo de secuencia común (ST11) de S. Enteritidis estuvo presente en todas las tres fuentes y mostraron el mismo patrón de resistencia antibiótica. Conclusión. S. Enteritidis ST11 constituye un vínculo entre el consumo/manipulación de huevos contaminados y gastroenteritis humana en Ibagué. Son necesarios estudios complementarios para conocer si otros serovares de Salmonella aislados de carne de pollo cruda también se asocian con la gastroenteritis humana.

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Section: Discussionmentioning

confidence: 99%

A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia

Fandiño

Verján

2019

biomedica

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“…HU–DNA interactions seem to be non-specific; therefore, we hypothesized that the differences in the genomic architecture between the serovars Typhimurium and Enteritidis may be significant for the different phenotypes. Differences among the genetic backgrounds of strains may also be linked to these results; a recently published genomic sequence of S. Enteritidis PT4 ( Milanez et al, 2016 ) can be used to elucidate these differences. Thus, further studies are necessary to clarify these differences.…”

Section: Discussionmentioning

confidence: 99%

“…This strain belongs to the Enteritidis serovar and is classified as phage type 4, designated as SEnPT4. It was previously isolated from a poultry farm in São Paulo state, and its genome sequence is deposited in GenBank under the accession ID: LTDW00000000 ( Milanez et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection

et al. 2018

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Salmonella enterica infection is a major public health concern worldwide, particularly when associated with other medical conditions. The serovars Typhimurium and Enteritidis are frequently associated with an invasive illness that primarily affects immunocompromised adults and children with HIV, malaria, or malnutrition. These serovars can also cause infections in a variety of animal hosts, and they are the most common isolates in poultry materials. Here, we described S. Enteritidis mutants, where hupA and hupB genes were deleted, and evaluated their potential use as live-attenuated vaccine candidates. In vitro, the mutants behaved like S. Typhimurium described previously, but there were some particularities in macrophage invasion and survival experiments. The virulence and immunogenicity of the mutant lacking both hupA and hupB (PT4ΔhupAB) were evaluated in a BALB/c mice model. This mutant was highly attenuated and could, therefore, be administrated at doses higher than 109 CFU/treatment, which was sufficient to protect all treated mice challenged with the wild-type parental strain with a single dose. Additionally, the PT4ΔhupAB strain induced production of specific IgG and IgA antibodies against Salmonella and TH1-related cytokines (IFN-γ and TNF-α), indicating that this strain can induce systemic and mucosal protection in the murine model. Additional studies are needed to better understand the mechanisms that lead to attenuation of the double-mutant PT4ΔhupAB and to elucidate the immune response induced by immunization using this strain. However, our data allow us to state that hupAB mutants could be potential candidates to be explore as live-attenuated vaccines.

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“…China (34)(35)(36)(37). Also, ST34 (Salmonella ser.4,5,12:i:-) was shared between chickens, farmers, and…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Spread of Antimicrobial-Resistant <i>Salmonella</i> from Poultry to Humans in Thailand

2021

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Food animal production is important for every country. Several antibiotic agents are used in poultry farming to reduce the economic losses arising from mostly untested, infectious diseases. This continued study was performed to determine the prevalence of antibiotic resistant Salmonella in broiler chickens, poultry farmers, and Salmonella-bacteremia patients. A total of 121 Salmonella isolates were collected from the Thai provinces of Khon Kaen (65 isolates), Ratchaburi (43 isolates), and Phayao (13 isolates). Salmonella from chicken showed a high rate of resistance to nalidixic acid and tetracycline. Sixty-four percent of Salmonella carried class 1 integron (intI1 gene positive). Among 121 Salmonella isolates, there were 15 serotypes, with Enteritidis the most common. A clonal relationship between the chicken and human isolates was demonstrated by three molecular typing methods: Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction; Pulsed-Field Gel Electrophoresis; and High-Throughput, Multilocus Sequence Typing. A spread of the sequence type 11 (ST11) clone was found between chickens and humans. This study revealed a large-scale Salmonella outbreak in Thailand, a link between resistant bacteria from poultry farms and vertical transmission through the food chain, and a horizontal transmission of resistance genes. These results can be used for future surveillance and monitoring. A c c e p t e d M a n u s c r i p t The study was approved by the Siriraj Institutional Review Board. Sample collection There were 121 Salmonella isolates from cloacal swabs of chickens, rectal swabs of chicken farmers, and blood isolates of patients, with 65 isolates from Khon Kaen, 43 from Ratchaburi, and 13 from Phayao, in 2012-2013. Only 81 isolates could be tested for their phenotype: 40 Salmonella chicken samples of the Ratchaburi Veterinary Research and Development Center only had total DNAs and plasmids for genotypic tests due to an agreement with this site. We additionally included 37 additional Salmonella isolated from bacteremic patients in Bangkok for High-Throughput, Multilocus Sequence Typing.

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Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm

Cited by 4 publications

References 14 publications

A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia

A common Salmonella Enteritidis sequence type from poultry and human gastroenteritis in Ibagué, Colombia

HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection

Spread of Antimicrobial-Resistant <i>Salmonella</i> from Poultry to Humans in Thailand

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