Whole-Genome Sequence of Streptococcus agalactiae Strain S13, Isolated from a Fish Eye from a Nile Tilapia Farm in Southern Brazil

Facimoto, César Toshio; Chideroli, Roberta Torres; Gonçalves, Daniela Dib; Carmo, Anderson Oliveira do; Kalaphotakis, Evanguedes; Pereira, Ulisses de Pádua

doi:10.1128/genomea.00917-17

Cited by 16 publications

(7 citation statements)

References 12 publications

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“…The plasma was diluted with phosphate buffered saline (PBS) at 1:1 ratio (50 μL PBS: 50 μL plasma solution) in the first well, after which the plasma was diluted serially in the other wells at a factor ratio of 1:2. Later, 50μL of Streptococcus agalactiae S13 serotype Ib (Facimoto et al, 2017) inactivated at a concentration of 1 × 10 8 colony forming unit (CFU) mL -1 was added to all wells, after which the microplate was incubated at 25°C for 18 h in a humid chamber. The bonding title is considered as the reciprocal of the last dilution for which agglutination is observed, that is, when aggregates can be observed at the well bottom with the naked eye (Silva et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

Nucleotides, β-glucans, ascorbic acid, α-tocoferol, and different concentrations of a vitamin-mineral premix promote growth of Nile tilapia juveniles

Sá,

Pierri,

Pereira

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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Feed additives, such as β-glucans, nucleotides, ascorbic acid (vitamin C), and α-tocopherol (vitamin E) can improve fish immunity and contribute to enhanced zootechnical performance of the fish. This study aimed to evaluate the effect of different concentrations of vitamin- mineral premix with or without the inclusion of an immunostimulant boost (β-glucans, nucleotides and vitamins C and E) on the zootechnical performance, hemato-immunological parameters, histological changes, and survival of Nile tilapia juveniles. To this end, isocaloric and isoproteic diets were prepared with three different concentrations of vitamin-mineral premix (1.0, 1.5, and 2.0 kg ton-1); additionally, the effects of 4% immunostimulant boost in the diet were examined considering six diets (without the immunostimulant boost: 0.1%, 0.15%, and 0.2%, and with the immunostimulant boost: 0.1% I, 0.15% I, and 0.2% I). We used 24 experimental units, each including 15 fish. Nile tilapia juveniles (1.88 g±0.25) were fed for 50 d with supplemented diets. Further, zootechnical indexes; histological changes in the liver, spleen, and intestine; intestinal morphology; and hemato-immunological parameters were evaluated. The fish that received supplementation showed higher zootechnical values, compared to those that did not receive the supplementation of immunostimulant reinforcement. Weight gain (30.45g±3.06), daily weight gain (0.60g±0.06), final weight (32.41g±3.15) and feed conversion (0.95±0.06) were higher in tilapia fed with an inclusion concentration of 0.2%. Hematological parameters were not affected by either the pre-mix concentrations or the booster of the immunostimulant. There was an increase in the number of intestinal folds, length of the fold, total area and number of goblet cells in the group supplemented with immunostimulant reinforcement. Supplementation with the immunostimulant promoted growth and improved intestinal morphology and immunological parameters of Nile tilapia juveniles.

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Section: Methodsmentioning

confidence: 99%

Nucleotides, β-glucans, ascorbic acid, α-tocoferol, and different concentrations of a vitamin-mineral premix promote growth of Nile tilapia juveniles

Sá,

Pierri,

Pereira

et al. 2023

Arq. Bras. Med. Vet. Zootec.

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“…The pathogenic bacteria S. agalactiae (S13) was isolated from moribund tilapia during a mortality outbreak in Paraná State, Brazil. This strain was obtained from the Fish Bacteriology Laboratory (LABBEP) and identified by molecular identification (Facimoto et al, 2017). For infection, bacteria were cultured in a BHI broth culture medium (HiMedia, Mumbai, India) at 28°C for 24 h. Subsequently, the culture was centrifuged at 1500 g for 10 min, and the supernatant was discarded.…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation of Viligen™ to Nile tilapia improves growth and gut morphology

et al. 2021

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We evaluated the growth, gut histomorphometry, selected immune responses and resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection when fed VILIGEN™, an additive blend containing sodium butyrate, dehydrated hydrolysed yeast and zinc proteinate. Increasing doses (0.00, 0.60, 1.20, 2.40 and 4.80 g kg−1) were added to a basal diet and randomly assigned to six groups, each with 28 fish. The fish were fed to satiation twice daily for 60 days, infected via gavage with S. agalactiae at 1.0 × 109 CFU ml−1, and mortality was recorded for 14 days. Dietary inclusion of VILIGEN™ promoted higher weight gain, gut‐fold perimeter and the number of goblet cells. Through polynomial regression, the optimal inclusion level for these variables was estimated at 2.80, 3.30 and 3.50 g kg−1, respectively. However, there was no clear effect of the additive on the immune responses tested before bacterial infection or in the survival after challenge. This is the first study on VILIGEN™ supplementation in fish diets. Higher growth and altered gut morphology were seen with the inclusion of VILIGEN™ in the range of 2.80 to 3.50 g kg−1 but the response to the bacterial challenge was not clearly affected.

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“…Subsequently, the plasma was serially diluted into the other wells at a ratio of 1:2. Then, 50 µL of inactivated Streptococcus agalactiae S13 serotype Ib [26] at a concentration of 1 × 10 8 colony-forming units (CFU) mL −1 was added to all wells. The microplate was then incubated at 25 • C for 18 h in a humid chamber.…”

Section: Immunological Parametersmentioning

confidence: 99%

The Zootechnical Performance, Health State Modulation, Morphology, and Intestinal Microbiome of Nile Tilapia Juveniles Fed with a Functional Blend of Immunostimulants Associated with a Diet High in Soybean Meal

Sá,

Jerônimo,

Soligo

et al. 2024

Fishes

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The aim of this study was to evaluate the effect of supplementing feed with a functional mixture of immunomodulators, including β-glucans, nucleotides, ascorbic acid, and alpha-tocopherol, associated with a diet with lower levels of animal protein (11.5%) and higher levels of soybean meal (43.5%), on the zootechnical performance, health, hematological and immunological parameters, intestinal morphology, centesimal composition, and intestinal microbiome of juvenile Nile tilapia (initial weight 1.88 g ± 0.25 g, mean ± standard deviation). Two isocaloric and isoproteic diets (35% crude protein) were formulated, one with the inclusion of the immunostimulant functional mixture (40 kg·t−1), composed of 150 mg·kg−1 of nucleotides, 1000 mg·kg−1 of β-glucans, 1000 mg·kg−1 of ascorbic acid (vitamin C), and 20 mg·kg−1 of alpha-tocopherol (vitamin E), and another without. The combined supplementation of nucleotides, β-glucans, ascorbic acid, and alpha-tocopherol resulted in a 59.95% increase in final weight, 64% weight gain, 66% daily gain, a 21.31% decrease in feed conversion rate, and double the retention of body protein. Supplementation also improved intestinal morphology and modulated the intestinal microbiome, increasing Chao-1 diversity. Transmission electron microscopy confirmed that fish fed with both diets exhibited intact intestinal mucosal membranes. Supplementation did not alter the hematological and immunological parameters, suggesting that there was no overstimulation of the fish’s immune system. This work allows us to evaluate the effect of reducing the use of animal protein in the diets of fish, along with the effects of nucleotides, β-glucans, ascorbic acid, and alpha-tocopherol. Together, these compounds can provide fish with the necessary tools to achieve optimal health and growth.

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Whole-Genome Sequence of Streptococcus agalactiae Strain S13, Isolated from a Fish Eye from a Nile Tilapia Farm in Southern Brazil

Abstract: Streptococcus agalactiae is an important pathogen to world aquaculture due to its high mortality rates in fish farms and consequent economic losses. Our study presents the complete genome sequence of strain S13, isolated from a tilapia farm outbreak in southern Brazil.

Cited by 16 publications

References 12 publications

Nucleotides, β-glucans, ascorbic acid, α-tocoferol, and different concentrations of a vitamin-mineral premix promote growth of Nile tilapia juveniles

Nucleotides, β-glucans, ascorbic acid, α-tocoferol, and different concentrations of a vitamin-mineral premix promote growth of Nile tilapia juveniles

Dietary supplementation of Viligen™ to Nile tilapia improves growth and gut morphology

The Zootechnical Performance, Health State Modulation, Morphology, and Intestinal Microbiome of Nile Tilapia Juveniles Fed with a Functional Blend of Immunostimulants Associated with a Diet High in Soybean Meal

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