Whole genome sequence of Streptomyces humi strain MUSC 119T isolated from intertidal soil

Abstract

Over the past few decades, microorganisms have made major contribution in natural product research, particularly those from the genus Streptomyces. Streptomyces humi MUSC 119T was previously isolated as novel streptomycete from mangrove soil in Malaysia. During the screening programme for bioactive strains, this strain was discovered to possess antioxidant activity – scavenging and reducing accumulation of free radicals in biochemical assays. Consequently, whole genome sequencing was performed to evaluate genomic potential of the strain. Based on our analysis, the genome size of MUSC 119T is described to be 10.01 Mbps with G + C content of 71.80%. Based on antiSMASH analysis, the strain possess great genomic potential, having nine biosynthetic gene clusters displaying high similarities to known gene clusters. These findings indicates that mangrove Streptomyces species like MUSC 119T may potentially play an important role in drug development process, while the availability of its whole genome sequences allows further manipulation to isolate and identify compound of interest.

Anticancer Drug Discovery from Microbial Sources: The Unique Mangrove Streptomycetes

“…Streptomycetes produce a high number of spores that are easily dispersed, and thus, it is projected that they have a cosmopolitan distribution [ 47 , 57 ]. Furthermore, the biosynthetic capacity of streptomycetes remains without rival given the fact that streptomycetes have a large genome size (>7 Mbp) [ 58 , 59 , 60 , 61 ], and some of them have been previously reported to own more than 20 gene clusters encoding predicted/known biosynthetic pathways [ 62 , 63 , 64 ]. Hence, this contributed to the unique capability of streptomycetes in the production of various novel and/or useful secondary metabolites with antioxidant, anticancer, antimicrobial, etc.…”

Critical review of fermentation and extraction of anti-Vibrio compounds from Streptomyces

“…Actinobacteria have been regarded as the most prolific producers in the microbial world [4][5][6][7][8] , especially from the genus Streptomyces [9,10] . The genus Streptomyces has responsible for the production of more than 70% of commercially important antibiotics [2,11] , as well as many bioactive compounds of pharmacological and agricultural interest [12][13][14][15][16][17][18][19][20][21][22] . The discovery of antibiotic from Actinobacteria is highly dependent on the effect of growth conditions on the production of secondary metabolites [23][24][25][26][27][28] .…”

Bioprospecting of Microbes for Valuable Compounds to Mankind

“…These challenges had led to intensely amplified in serious demand for new structures in pharmacology, hence propelled the investigation of new habitats with poorly explored areas and uncommon environments to become vital for the discovery of novel bacteria (e.g. Actinobacteria) and useful metabolites [44][45][46][47][48][49][50][51][52][53][54][55] . Reports from poorly explored areas from these regions (e.g.…”