Whole-Genome Sequences of Chlamydia psittaci Strain HJ, Isolated from Meat Pigeons with Severe Respiratory Distress and High Mortality

Zhang, Qiang; Wu, Zongxue; Sun, Ruixia; Chen, Jun; Han, Er; Zhang, Yinxin; Ling, Yong; Gong, Yanping; Li, Dongfang; Wu, Honglong; He, Cheng; Ni, Peixiang

doi:10.1128/genomea.00035-15

Cited by 10 publications

(6 citation statements)

References 11 publications

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“…A highly virulent HJ strain of C. psittaci was isolated from diseased pigeons 11 while avian influenza virus (H9N2/chicken/Shandong/2011) 3 and Ornithobacterium rhinotracheale (ORT/chicken/Shandong/2011) 3 were isolated from the broilers with hemorrhagic pneumonia.…”

Section: Methodsmentioning

confidence: 99%

“…These pathogens should be considered when developing prevention strategies for respiratory disease in broilers 7 , 8 . Moreover, high seroprevalence of C. psittaci has been determined in Belgium, France and China with seropositive rates of 96%, 90% and 77.8%, respectively in broiler flocks 9 – 11 . A virulent C. psittaci strain has been isolated from meat pigeon farms with an outbreak involving severe respiratory signs 11 .…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, high seroprevalence of C. psittaci has been determined in Belgium, France and China with seropositive rates of 96%, 90% and 77.8%, respectively in broiler flocks 9 – 11 . A virulent C. psittaci strain has been isolated from meat pigeon farms with an outbreak involving severe respiratory signs 11 . Importantly, C. psittaci is also a zoonotic pathogen in humans and poses a potentially serious human health hazard 12 .…”

Section: Introductionmentioning

confidence: 99%

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Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens

Chen

Qiang

Zuo

et al. 2017

Sci Rep

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Since 2007, most areas of China have seen outbreaks of poultry airsacculitis, which causes hugely economic losses to the poultry industry. However, there are no effective measures to combat the problem. In this study, 105 rations were collected to isolate Aspergillus spp. from the diseased farms. In subsequent experiments, SPF chickens were inoculated with Ornithobacterium rhinotracheale (ORT), Chlamydia psittaci (C. psittaci) and Aspergillus fumigatus (A. fumigatus), and mortality rate, body weight gain and lesion score were evaluated. Of these ration samples, 63 (60.0%) were A. fumigates, 21 (20.0%) were Aspergillus niger (A. niger) and 11 (10.5%) were Aspergillus candidus (A. candidus). Furthermore, SPF birds infected with C. psittaci, ORT, H9N2 virus and A. fumigatus conidia exhibited a mortality rate of 40%, while simultaneous co-infection with C. psittaci, ORT and A. fumigatus resulted in a mortality rate of 20%. The avian airsacculitis was manifested in the C. psittaci + ORT/A. fumigatus, C. psittaci + H9N2 + ORT/A. fumigatus and C. psittaci + H9N2/A. fumigatus groups while others had transient respiratory diseases without mortality. Our survey indicates that feed-borne A. fumigatus is prevalent in poultry rations. The combination of C. psittaci, ORT, H9N2 and A. fumigatus conidia contributes to the replication of avian airsacculitis by aggravating the severe damage to the air sacs and lungs of chickens.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens

Chen

Qiang

Zuo

et al. 2017

Sci Rep

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“…Low virulence strain CB3 and high virulence HJ strain of C. psittaci were isolated from free-living birds 27 and diseased pigeons 28 . AIV H9N2/chicken/Shandong/2011 was isolated from broilers as described previously 11 .…”

Section: Methodsmentioning

confidence: 99%

Chlamydia psittaci infection increases mortality of avian influenza virus H9N2 by suppressing host immune response

Chen

Qiang

Zhang

et al. 2016

Sci Rep

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Avian influenza virus subtype H9N2 (H9N2) and Chlamydia psittaci (C. psittaci) are frequently isolated in chickens with respiratory disease. However, their roles in co-infection remain unclear. We tested the hypothesis that C. psittaci enhances H9N2 infection through suppression of host immunity. Thus, 10-day-old SPF chickens were inoculated intra-tracheally with a high or low virulence C. psittaci strain, and were simultaneously vaccinated against Newcastle disease virus (NDV). Significant decreases in body weight, NDV antibodies and immune organ indices occurred in birds with the virulent C. psittaci infection, while the ratio of CD4+/CD8+ T cells increased significantly compared to that of the lower virulence strain. A second group of birds were inoculated with C. psittaci and H9N2 simultaneously (C. psittaci+H9N2), C. psittaci 3 days prior to H9N2 (C. psittaci/H9N2), or 3 days after H9N2 (H9N2/C. psittaci), C. psittaci or H9N2 alone. Survival rates were 65%, 80% and 90% in the C. psittaci/H9N2, C. psittaci+H9N2 and H9N2/C. psittaci groups, respectively and respiratory clinical signs, lower expression of pro-inflammatory cytokines and higher pathogen loads were found in both C. psittaci/H9N2 and C. psittaci+H9N2 groups. Hence, virulent C. psittaci infection suppresses immune response by inhibiting humoral responses and altering Th1/Th2 balance, increasing mortality in H9N2 infected birds.

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“…MLST provides higher discrimination and is robust, but requires time-consuming sequencing of seven gene targets. With the increase in number of whole genome sequences (WGS) available for C. psittaci strains [ 12 , 13 , 14 , 15 , 16 ], it is now possible to apply single nucleotide polymorphisms (SNPs) for the establishment of a phylogenetic tree pointing out true relationship between C. psittaci isolates. In our study, we aimed to develop a highly discriminative and user-friendly method where the work comprises three parts: (i) to identify clusters, based on SNP analysis, (ii) to compare those with MLST- and omp A-based topologies and (iii) to develop a rapid typing tool based on the identification of relevant SNPs by PCR/high-resolution melting (HRM) technology for straightforward genotyping of C. psittaci from isolates and clinical samples.…”

Section: Introductionmentioning

confidence: 99%

A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification

et al. 2021

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Chlamydia (C.) psittaci is the causative agent of avian chlamydiosis and human psittacosis. In this study, we extracted single-nucleotide polymorphisms (SNPs) from the whole genome sequences of 55 C. psittaci strains and identified eight major lineages, most of which are host-related. A combined PCR/high-resolution melting (HRM) assay was developed to screen for eight phylogenetically informative SNPs related to the identified C. psittaci lineages. The PCR-HRM method was validated on 11 available reference strains and with a set of 118 field isolates. Overall, PCR-HRM clustering was consistent with previous genotyping data obtained by ompA and/or MLST analysis. The method was then applied to 28 C. psittaci-positive samples from animal or human cases. As expected, PCR-HRM typing results from human samples identified genotypes linked to ducks and pigeons, a common source of human exposure, but also to the poorly described Mat116-like genotype. The new genotyping method does not require time-consuming sequencing and allows a quick identification of the source of infection.

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Whole-Genome Sequences of Chlamydia psittaci Strain HJ, Isolated from Meat Pigeons with Severe Respiratory Distress and High Mortality

Cited by 10 publications

References 11 publications

Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens

Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens

Chlamydia psittaci infection increases mortality of avian influenza virus H9N2 by suppressing host immune response

A New SNP-Based Genotyping Method for C. psittaci: Application to Field Samples for Quick Identification

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