Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Lloyd, Nicole A.; Nazaret, Sylvie; Barkay, Tamar

doi:10.1016/j.marpolbul.2018.07.051

Cited by 22 publications

(6 citation statements)

References 67 publications

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“…That finding is in line with previously reported studies on mercury's impact on antibiotic-resistance selection (Skurnik et al, 2010). Lloyd et al (2018) showed that mercury-exposed fish microbiomes in the environment were highly susceptible to containing antibiotic-multiresistant bacteria, compared to unexposed controls. Coresistance due to mercury is apparently not new, since Wardwell et al (2009) showed that mercury-exposed indigenous bacterial strains in 2000-year-old sphagnum core samples had a multidrug-resistance phenotype.…”

Section: Heavy Metalssupporting

confidence: 92%

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Henriot

Buelow

Petit

et al. 2021

Preprint

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Antibiotic-resistance emergence and selection have become major public health issues globally. The presence of antibiotic resistant bacteria (ARB) in natural and anthroposophical environments threatens to compromise the sustainability of care in human and animal populations. This study was undertaken to develop a simple model formalizing the selective impact of antibiotics and pollutants on the dynamics of bacterial resistance in water and use the model to analyze longitudinal spatiotemporal data collected in hospital and urban wastewaters. Longitudinal-sampling data were collected between 2012 and 2015 in four different locations in Haute-Savoie, France: hospital and urban wastewaters, before and after water-treatment plants. Concentration in three different types of compounds: 1) heavy metals 2) antibiotics and 3) surfactants; and abundance of 88 individual genes and mobile genetic elements, mostly conferring resistance to antibiotics, were simultaneously collected. A simple hypothesis-driven model describing the weekly ARB dynamics was proposed to fit available data by assuming normalized gene abundance to be proportional to ARB populations in water. Compounds impacts on the dynamics of 17 genes found in multiple sites were estimated. We found that while mercury and vancomycin had relevant effects on ARB dynamics, respectively positively affecting the dynamics of 10 and 12 identified genes, surfactants antagonistically affected genes dynamics (identified for three genes). This simple model enables analyzing the relationship between resistance-gene persistence in aquatic environments and specific compounds inherent to human activities. Applying our model to longitudinal data, we identified compounds that act as co-selectors for antibiotic resistance.HighlightsWe analyzed longitudinal wastewater resistance genes and environmental dataWe developed a simple hypothesis-driven model to assess resistance selectionMercury and vancomycin were key drivers of antibiotic resistance in wastewater

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Section: Heavy Metalssupporting

confidence: 92%

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Henriot

Buelow

Petit

et al. 2021

Preprint

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“… 2017 ). A study assessing the link between antibiotic and metal resistance in marine bacterial isolates found that most resistance genes were chromosomally located and identified homologs of efflux pump genes which are commonly expressed to overcome pressure from heavy metals and may further contribute to resistant phenotypes (Lloyd, Nazaret and Barkay 2018 ). Similar results were obtained among bacterial communities sampled from hot springs (Jardine, Mavumengwana and Ubomba-Jaswa 2019 ).…”

Section: Amr and Pressure From Antibiotic Metabolites And Heavy Metalsmentioning

confidence: 99%

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

Niegowska

Sanseverino

Navarro

et al. 2021

FEMS Microbiology Ecology

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The spread of antibiotic resistance in the water environment has been widely described. However, still many knowledge gaps exist regarding the selection pressure from antibiotics, heavy metals and other substances present in surface waters as a result of anthropogenic activities, as well as the extent and impact of this phenomenon on aquatic organisms and humans. In particular, the relationship between environmental concentrations of antibiotics and the acquisition of ARGs by antibiotic-sensitive bacteria as well as the impact of heavy metals and other selective agents on antimicrobial resistance (AMR) need to be defined. Currently, established safety values are based on the effects of antibiotic toxicity neglecting the question of AMR spread. In turn, risk assessment of antibiotics in waterbodies remains a complex question implicating multiple variables and unknowns reinforced by the lack of harmonized protocols and official guidelines. In the present review, we discussed current state-of-the-art and the knowledge gaps related to pressure exerted by antibiotics and heavy metals on aquatic environments and their relationship to the spread of AMR. Along with this latter, we reflected on i) the risk assessment in surface waters, ii) selective pressures contributing to its transfer and propagation, and iii) the advantages of metagenomics in investigating AMR. Furthermore, the role of microplastics in co-selection for metal and antibiotic resistance, together with the need for more studies in freshwater are highlighted.

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“…Burkholderia cepacia has been associated with lesions of the oral cavity of these animals including ulcerative stomatitis, and traumatic skin lesions. Shewanella putrefaciens has been regarded as a severe opportunistic bacterium [18,[39][40][41][42] and was described in one case of septicemia in green sea turtles (Chelonia mydas) with underlying fibropapillomatosis [12]. Citrobacter sp.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance and Virulence Profiles of Gram-Negative Bacteria Isolated from Loggerhead Sea Turtles (Caretta Caretta) of the Island of Maio, Cape Verde

Fernandes

Grilo

Cunha

et al. 2021

Preprint

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Background: Several studies detected high levels of antibiotic-resistance in loggerhead sea turtles (Caretta caretta) and pointed this species as prime reservoirs of antibiotic-resistant bacteria and carriers of potentially pathogenic bacteria. This study aimed to characterize, for the ﬁrst time, the Gram-negative aerobic microbiota of the Cape Verdean loggerhead subpopulation. Cloacal, oral and egg content swab samples from 33 nesting loggerheads (n = 99) of the Island of Maio were analysed regarding the presence of Gram-negative bacteria and their antibiotic resistance and virulence proﬁles. Results: Shewanella putrefaciens (27.78%), Morganella morganii (22.22%) and Vibrio alginolyticus (22.22%) were the most prevalent species isolated from the animals under study. A low incidence of antibiotic-resistant bacteria (26%) was detected, and no multidrug-resistant isolates were identiﬁed. Non-Enterobacteriaceae isolates presented the most complex virulence proﬁles, revealing the ability to produce hemolysins (100%), DNases (89%), lipases (79%), proteases (53%), lecithinases (21%), gelatinases (16%), and also bioﬁlms (74%). Moreover, higher virulence indices were obtained for turtles with high parasite intensities compared with apparently healthy animals, and a positive correlation between antibiotic resistance and virulence was observed. Conclusions: Results suggest that this loggerhead population may be less exposed to antimicrobial compounds, probably due to the low anthropogenic impact observed in both their nesting (the Island of Maio) and foraging sites. Nevertheless, the presence of potentially pathogenic bacteria expressing virulence factors may threat both sea turtles’ and humans’ health.

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Whole genome sequences to assess the link between antibiotic and metal resistance in three coastal marine bacteria isolated from the mummichog gastrointestinal tract

Cited by 22 publications

References 67 publications

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Modelling the impact of urban and hospital wastewaters eco-exposomes on the antibiotic-resistance dynamics

Knowledge gaps in the assessment of antimicrobial resistance in surface waters

Antibiotic Resistance and Virulence Profiles of Gram-Negative Bacteria Isolated from Loggerhead Sea Turtles (Caretta Caretta) of the Island of Maio, Cape Verde

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