Whole Genome Sequencing, Antibiotic Resistance, and Epidemiology Features of Nontyphoidal Salmonella Isolated From Diarrheic Children: Evidence From North China

Abstract

Nontyphoidal Salmonella (NTS) in children remains a growing burden on public health and often causes children to be hospitalized with diarrheic symptoms. In this work, 260 strains of human Salmonella isolated from Jilin, China were characterized by serotypes and antimicrobial resistance using whole genome sequencing (WGS). The most prevalent serotype was Salmonella enteritidis (47.3%), followed by S. I 4,[5],12:i:- (33.1%), and Salmonella Typhimurium (7.3%). Furthermore, the consistency between resistance phenotype and genotype was confirmed. Similarly, strains harbored blaTEM−1B and tetA genes were detected, which verified the level of resistant phenotype in β-lactams and tetracyclines. The presence of a single mutation in parC, gyrA, and qnrS1 genes corresponding to quinolones was also observed. In our work, multilocus sequence typing (MLST) and core genome multilocus sequence typing (cgMLST) were found to have a high resolution to molecular traceability, and the combination of both was conducive to practical application in an actual situation. Taking all of this into account, we suggested that the comprehensive surveillance of Salmonella infection in children should be carried out to monitor antimicrobial-resistant trends from various sources and to alert on outbreaks of foodborne diseases to protect public health.

Antimicrobial resistance and genomic investigation of Salmonella isolated from retail foods in Guizhou, China

“…However, all 102 strains were successfully sequenced. Consequently, all 102 strains were utilized for the further antimicrobial susceptibility testing and whole genomic sequencing ( Zhao et al, 2022 ; Petrin et al, 2023 ). Among these, 50 strains were from the northern region, and 52 were from the southern region.…”

Interpretable machine learning-derived nomogram model for early detection of persistent diarrhea in Salmonella typhimurium enteritis: A propensity score matching based case-control study

“…Generally, S. typhimurium infections are uncomplicated and do not require special treatment; however, they can become serious and even life-threatening in young children and immunocompromised populations. Children younger than ve years of age are especially susceptible to S. typhimurium, with main clinical manifestations of acute fever episodes, vomiting, and diarrhea; furthermore, dehydration and bloody purulent stool can present in severe cases [5,6]. S. typhimurium-caused diarrhea usually lasts one week to ten days [2,7].…”

Salmonella Enteritidis activates inflammatory storm via SPI-1 and SPI-2 to promote intracellular proliferation and bacterial virulence

“…Molecular analysis revealed how S. Typhimurium manipulates host cell signaling through effector proteins. Recent studies show that S. Enteritidis is the most prevalent serovar among Salmonella isolates from children with diarrhea ( Zhao et al., 2022 ). However, the contribution and related signaling pathways of SPI-1 and SPI-2 in inflammation caused by S. Enteritidis during the course of infection remain unclear.…”