Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

Dadar, Maryam; Brangsch, Hanka; Alamian, Saeed; Neubauer, Heinrich; Wareth, Gamal

doi:10.1016/j.onehlt.2023.100483

Cited by 7 publications

(4 citation statements)

References 58 publications

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“…As a result, resistance and virulence mechanisms at the genomic, proteomic, and transcriptome levels trigger the alert about an increase in the number of residence genes. The stealthy Brucella pathogen has different AMR mechanisms such as enzymatic activation and inactivation of the antimicrobial agent, efflux pumps, replacement of protein or alteration occurring in cell wall charge via protein, drug target modification and resistance genes regulating the gene expression (Biswas et al 2008 ; Dadar et al 2023 ). DNA gyrase enzyme (gyrA and gyrB) has the primary role in DNA supercoiling, and inhibition of this activity using quinolones or fluoroquinolones generation is combined with rapid killing of the pathogenic Brucella (Spencer and Panda 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt

Elrashedy,

Nayel,

Salama

et al. 2024

J Mol Evol

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Brucellosis is a notifiable disease induced by a facultative intracellular Brucella pathogen. In this study, eight Brucella abortus and eighteen Brucella melitensis strains from Egypt were annotated and compared with RB51 and REV1 vaccines respectively. RAST toolkit in the BV-BRC server was used for annotation, revealing genome length of 3,250,377 bp and 3,285,803 bp, 3289 and 3323 CDS, 48 and 49 tRNA genes, the same number of rRNA (3) genes, 583 and 586 hypothetical proteins, 2697 and 2726 functional proteins for B. abortus and B. melitensis respectively. B. abortus strains exhibit a similar number of candidate genes, while B. melitensis strains showed some differences, especially in the SRR19520422 Faiyum strain. Also, B. melitensis clarified differences in antimicrobial resistance genes (KatG, FabL, MtrA, MtrB, OxyR, and VanO-type) in SRR19520319 Faiyum and (Erm C and Tet K) in SRR19520422 Faiyum strain. Additionally, the whole genome phylogeny analysis proved that all B. abortus strains were related to vaccinated animals and all B. melitensis strains of Menoufia clustered together and closely related to Gharbia, Dameitta, and Kafr Elshiek. The Bowtie2 tool identified 338 (eight B. abortus) and 4271 (eighteen B. melitensis) single nucleotide polymorphisms (SNPs) along the genomes. These variants had been annotated according to type and impact. Moreover, thirty candidate genes were predicted and submitted at GenBank (24 in B. abortus) and (6 in B. melitensis). This study contributes significant insights into genetic variation, virulence factors, and vaccine-related associations of Brucella pathogens, enhancing our knowledge of brucellosis epidemiology and evolution in Egypt.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt

Elrashedy,

Nayel,

Salama

et al. 2024

J Mol Evol

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“…Brucellosis is one of the most important zoonotic diseases prevailing in the large and small ruminants of the Middle East, including Iran [ 10 , 16 , 26 ]. Vaccination is vital for preventing and eradicating infections and is the most economical control component [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Abnaroodheleh¹,

Emadi²,

Dashtipour³

et al. 2023

Heliyon

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“…The species B. suis belongs to core clade of Brucella phylogeny 70 and in contrast to other Brucella species, e.g. B. melitensis 37 , 71 , strains of the same biovars form distinct clusters in the polytomy. From the beginnings of Brucella genotyping, allele-based typing methods already indicated that B. suis bv.…”

Section: Discussionmentioning

confidence: 99%

“…individual strains may differ in as little as 0.15% of their genome from each other. Therefore, single nucleotide substitutions have to be employed for the reconstruction of strain similarities, that help identifying the infection source 36 , 37 . This analysis heavily depends on the availability of genome sequence data, with which an isolate’s sequence can be compared.…”

Section: Introductionmentioning

confidence: 99%

Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5

Brangsch,

Horstkotte,

Melzer

2023

Sci Rep

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Human brucellosis cases are rare in non-endemic countries, such as Germany, where infections are predominantly caused by Brucella melitensis. The German National Reference Laboratory for Bovine, Porcine, Ovine and Caprine Brucellosis received a suspected Brucella sp. isolate from a patient for identification. Bacteriological tests and PCR-based diagnostics showed the isolate to be B. suis, but did not yield cohesive results regarding the biovar. Whole genome sequencing and subsequent genotyping was employed for a detailed characterization of the isolate and elucidating the reason for failure of the diagnostic PCR to correctly identify the biovar. The isolate was found to be B. suis bv. 5, a rare biovar with limited geographical distribution primarily found in the Northern Caucasus. Due to a deletion in one of the target regions of the diagnostic PCR, the isolate could not be correctly typed. Based on in silico genotyping it could be excluded that the isolate was identical to one of the B. suis bv. 5 reference strains. Here, we report a rare case of a B. suis bv. 5 field isolate. Furthermore, by reporting this finding, we want to make practitioners aware of possible misinterpretation of PCR results, as it cannot be excluded that the detected deletion is common among the B. suis bv. 5 community, as there is currently a lack of field isolates.

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Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

Cited by 7 publications

References 58 publications

Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt

Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt

Shedding rate of Brucella spp. in the milk of seropositive and seronegative dairy cattle

Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5

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