Whole-genome sequencing of a biocontrol Myxococcus xanthus R31 isolate and comparative genomic analysis

Dong, Honghong; Gao, Ruixiang; Dong, Yijie; Yao, Qing; Zhu, Honghui

doi:10.1016/j.gene.2023.147286

Cited by 10 publications

(6 citation statements)

References 86 publications

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“…CAZymes include glycosyltransferases (GTs), glycoside hydrolases (GHs), carbohydrate esterases (CEs), auxiliary activities (AAs), carbohydrate-binding modules (CBMs), and polysaccharide lyases (PLs). CAZymes can modify the glycosidic bonds of carbohydrates and are important basic functional units in carbohydrate metabolism pathways ( Dong et al, 2023 ). GHs hydrolyze glycosidic bonds and play an important role in the hydrolysis and synthesis of sugars and glycoconjugates in organisms ( Kaushal and Singh, 2020 ).…”

Section: Enzymesmentioning

confidence: 99%

Active substances of myxobacteria against plant diseases and their action mechanisms

Zhang,

Bao,

et al. 2024

Front. Microbiol.

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Myxobacteria have a complex life cycle and unique social behavior, and obtain nutrients by preying on bacteria and fungi in soil. Chitinase, β-1,3 glucanase and β-1,6 glucanase produced by myxobacteria can degrade the glycosidic bond of cell wall of some plant pathogenic fungi, resulting in a perforated structure in the cell wall. In addition, isooctanol produced by myxobacteria can lead to the accumulation of intracellular reactive oxygen species in some pathogenic fungi and induce cell apoptosis. Myxobacteria can also perforate the cell wall of some plant pathogenic oomycetes by β-1,3 glucanase, reduce the content of intracellular soluble protein and protective enzyme activity, affect the permeability of oomycete cell membrane, and aggravate the oxidative damage of pathogen cells. Small molecule compounds such as diisobutyl phthalate and myxovirescin produced by myxobacteria can inhibit the formation of biofilm and lipoprotein of bacteria, and cystobactamids can inhibit the activity of DNA gyrase, thus changing the permeability of bacterial cell membrane. Myxobacteria, as a new natural compound resource bank, can control plant pathogenic fungi, oomycetes and bacteria by producing carbohydrate active enzymes and small molecular compounds, so it has great potential in plant disease control.

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Section: Enzymesmentioning

confidence: 99%

Active substances of myxobacteria against plant diseases and their action mechanisms

Zhang,

Bao,

et al. 2024

Front. Microbiol.

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“…A protein with lysozyme-like-activity (LlpM) able to induce cell lysis has been identified, although it is not essential for this process, reinforcing the idea that cell-wall lytic activity of the M. xanthus secretome is a multifactorial process with several hydrolytic enzymes involved ( Arend et al, 2021 ). In fact, secretion of hydrolytic enzymes has shown to drive predation in natural isolates with genomes specially enriched in this type of enzymes ( Dong et al, 2022a , 2023 ).…”

Section: Hydrolytic Enzymesmentioning

confidence: 99%

Myxococcus xanthus predation: an updated overview

Contreras-Moreno,

Pérez,

Muñoz-Dorado

et al. 2024

Front. Microbiol.

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Bacterial predators are widely distributed across a variety of natural environments. Understanding predatory interactions is of great importance since they play a defining role in shaping microbial communities in habitats such as soils. Myxococcus xanthus is a soil-dwelling bacterial predator that can prey on Gram-positive and Gram-negative bacteria and even on eukaryotic microorganisms. This model organism has been studied for many decades for its unusual lifecycle, characterized by the formation of multicellular fruiting bodies filled with myxospores. However, less is known about its predatory behavior despite being an integral part of its lifecycle. Predation in M. xanthus is a multifactorial process that involves several mechanisms working synergistically, including motility systems to efficiently track and hunt prey, and a combination of short-range and contact-dependent mechanisms to achieve prey death and feed on them. In the short-range attack, M. xanthus is best known for the collective production of secondary metabolites and hydrolytic enzymes to kill prey and degrade cellular components. On the other hand, contact-dependent killing is a cell-to-cell process that relies on Tad-like and type III secretion systems. Furthermore, recent research has revealed that metals also play an important role during predation, either by inducing oxidative stress in the prey, or by competing for essential metals. In this paper, we review the current knowledge about M. xanthus predation, focusing on the different mechanisms used to hunt, kill, and feed on its prey, considering the most recent discoveries and the transcriptomic data available.

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“…Myxobacteria produce extracellular enzymes with carbohydrate-active enzymes (CAZymes), mainly glycosyltransferases (GTs), glycoside hydrolases (GHs), carbohydrate-binding modules (CBMs) and polysaccharide (PLs), etc. CAZymes can modify the glycosidic bonds of carbohydrates and are important basic functional units in carbohydrate metabolism pathways, while GHs can degrade the glycosidic bonds of peptidoglycan in the cell wall (Dong et al, 2023 ). When myxobacteria prey on prey, they can kill microorganisms and lyse cells by producing metabolites such as antibiotics, cell wall degrading enzymes, lipases, nucleases, polysaccharases, and proteases, thereby clearing biological macromolecules.…”

mentioning

confidence: 99%

“…CAZymes include glycosyltransferases (GTs), glycoside hydrolases (GHs), carbohydrate esterases (CEs), auxiliary activities (AAs), carbohydrate-binding modules (CBMs), and polysaccharide lyases (PLs). CAZymes can modify the glycosidic bonds of carbohydrates and are important basic functional units in carbohydrate metabolism pathways (Dong et al, 2023 ). GHs hydrolyze glycosidic bonds and play an important role in the hydrolysis and synthesis of sugars and glycoconjugates in organisms (Kaushal and Singh, 2020 ).…”

mentioning

confidence: 99%

“…The MEROPS database of strain R31 genome had annotated 274 proteins, including 132 metalloproteases and 107 serine proteases. Three M36 metalloproteases were identified in the R31 genome that may contribute to the extracellular digestion of proteins during predatory behavior (Dong et al, 2023 ). Proteins of the M23 family were endopeptidases that cleaved bacterial cell wall peptidoglycan by degrading the peptide bonds of cross-linked peptides (Odintsov et al, 2004 ).…”

mentioning

confidence: 99%

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Corrigendum: Active substances of myxobacteria against plant diseases and their action mechanisms

Zhang,

Bao,

et al. 2024

Front. Microbiol.

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Whole-genome sequencing of a biocontrol Myxococcus xanthus R31 isolate and comparative genomic analysis

Cited by 10 publications

References 86 publications

Active substances of myxobacteria against plant diseases and their action mechanisms

Active substances of myxobacteria against plant diseases and their action mechanisms

Myxococcus xanthus predation: an updated overview

Corrigendum: Active substances of myxobacteria against plant diseases and their action mechanisms

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