Whole-Genome Sequencing of a
            <i>Mycobacterium orygis</i>
            Strain Isolated from Cattle in Chennai, India

Refaya, Ahmed Kabir; Kumar, Narender; Raj, Dhinakar; Maroudam, Veerasamy; Balaji, S.; Shanmugam, Sivakumar; Rajendran, Ananthi; Tripathy, Srikanth; Swaminathan, Soumya; Peacock, Sharon J.; Palaniyandi, Kannan

doi:10.1128/mra.01080-19

Cited by 17 publications

(13 citation statements)

References 14 publications

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“…However, recent discoveries have challenged this notion making it necessary to reassess the cause and effect of bTB in humans and animals [ 19 ]. The study by Refaya et al in 2019 reported the isolation of M. orygis strain in comparative intradermal test (CIT)- positive cattle from a farm in Chennai using whole genome sequencing [ 20 ]. A cross-sectional surveillance study done in three farms in Chennai to test for tuberculosis among cattle and animal handlers revealed that four cattle and six animal handlers were infected with the same strain of M. tuberculosis .…”

Section: Need For Btb Control Policies In India- the Human Frontmentioning

confidence: 99%

Bovine tuberculosis in India: The need for One Health approach and the way forward

Ramanujam

Palaniyandi

2023

One Health

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Section: Need For Btb Control Policies In India- the Human Frontmentioning

confidence: 99%

Bovine tuberculosis in India: The need for One Health approach and the way forward

Ramanujam

Palaniyandi

2023

One Health

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“…Hence, molecular detection techniques like PCR and real-time PCR gains importance owing to the rapidity and sensitivity of the tests to detect MTBC. Though M. bovis had been reported to be the predominant cause of bTB, the presence of other organisms were not excluded, as M. tuberculosis (Srivastava et al, 2008;Sweetline Anne et al, 2017), M. orygis (Refaya et al, 2019), M. caprae and M. pinnepedi (OIE, 2009) also had been reported to cause bTB. Hence the positive samples obtained in the present study have to be further analysed for species confirmation.…”

Section: Despitementioning

confidence: 99%

“…. Similarly, tuberculosis in bovines caused by Mycobacterium orygis (M. orygis) and Mycobacterium caprae (M. caprae) of MTBC are also gaining importance (OIE, 2009;Refaya et al, 2019).…”

mentioning

confidence: 99%

Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples

Sarika¹,

Mani²,

Mini³

et al. 2022

Journal of Veterinary and Animal Sciences

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Bovine tuberculosis (bTB), predominantly caused by Mycobacterium bovis, is one of the most neglected zoonotic diseases of cattle. The lack of documented information on bTB is one of the most important hurdles in controlling the disease. The present study was carried out to detect the presence of Mycobacterium tuberculosis complex (MTBC) from blood samples of cattle presented for slaughter, using polymerase chain reaction. The study revealed presence of MTBC in seven out of 50 animals (fourteen per cent) with symptoms suggestive of bTB. The detection of the organism emphasises the need of further prevalence studies in Kerala, to implement proper control strategies.

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“…Mycobacterium orygis is a member of Mycobacterium tuberculosis complex (MTBC) capable of causing disease in both humans and animals as reported by several studies (Refaya et al., 2019; Van Ingen et al., 2012). MTBC is composed of several highly genetically related species that have adapted to both human and animal hosts possessing the ability to propagate and transmit in a variety of wild and domesticated animals such as goats, pigs, buffaloes, dogs, primates, badgers, deer, possums and bison (Malone & Gordon, 2017).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tuberculosis caused by Mycobacterium orygis in wild ungulates in Chennai, South India

Refaya

Ramanujam

Ramalingam³

et al. 2022

Transbounding Emerging Dis

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We report the isolation of Mycobacterium orygis, a member of Mycobacterium tuberculosis complex (MTBC), from two black bucks (Antelope cervicapra) and one spotted deer (Axis axis) from the Guindy National Park forest range in Chennai, India. Lung tissue and lymph node samples collected during post-mortem examination were processed using NaOH method and cultured in solid and liquid media. DNA extracted from the cultured isolates was used to amplify the mpt64 gene by specific primers and the band visualized at 240 bps confirmed the isolates as a member of MTBC. Further examination of these isolates by spoligotyping and whole-genome sequencing confirmed the isolates as M. orygis and the phylogenetic tree revealed their well-clustered position with other M. orygis isolates around the globe. The deletion of RD7-RD10, RDOryx_1, RDOryx_4, RD12Oryx, RD301 and RD315 further substantiated these isolates as M.orygis. The exact source of infection in animals was untraceable and the pairwise comparison of the genomes based on single-nucleotide polymorphisms difference did not detect any events of transmission within the affected animals. Nevertheless, it would be wise to take into account the environment where there exists a high chance of transmission due to the increased human-animal interaction. Since it is well known that the pathogen is capable of causing infection in both human and animal hosts, systematic surveillance and screening of spotted deer, black buck as well as humans in the vicinity is essential for successful implementation of the One Health approach.

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Whole-Genome Sequencing of a Mycobacterium orygis Strain Isolated from Cattle in Chennai, India

Abstract: Here, we report the isolation of Mycobacterium orygis from dairy cattle in Chennai, India. Spoligotyping assigned the isolate to spoligotype 587 (ST587), which belongs to M. orygis. This species was confirmed as M. orygis using whole-genome sequencing.

Cited by 17 publications

References 14 publications

Bovine tuberculosis in India: The need for One Health approach and the way forward

Bovine tuberculosis in India: The need for One Health approach and the way forward

Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples

Tuberculosis caused by Mycobacterium orygis in wild ungulates in Chennai, South India

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