Whole-Genome Sequencing of Alcaligenes faecalis HZ01, with Potential to Inhibit Nontuberculous Mycobacterial Growth

Fernandes, Henry Marcel Zalona; Conceição, Emilyn Costa; Silva, Sandro Patroca da; Machado, Edson; Sisco, Maria Carolina; Sharma, Abhinav; Lima, Karla Valéria Batista; Conceição, Marília Lima da; Carvalho, Ana Carolina da Silva; Miranda, Karla Rodrigues; Duarte, Rafael Silva; Alviano, Daniela Sales; Dias, Rubens Clayton da Silva

doi:10.1128/mra.00521-21

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The HA-negative mutant strain, A. ammonioxydans 2-29, showed no antagonism and reconstituting this mutant with the intact dnfR gene restored the ability to inhibit the growth of V4 cells, indicating that HA was the key molecule in this process. Since other Alcaligenes species are potential candidates in the production of antibacterial substances (Felestrino et al, 2020;Fernandes et al, 2021), it is tempting to speculate that other antagonistic secondary metabolites existed. The degradation of HA beyond ∼24 h of culture, while some antagonism could still be observed beyond this time point suggested that this may be the case especially during the late growth stage of HO-1.…”

Section: Discussionmentioning

confidence: 99%

“…Many B. velezensis strains gained practical application in promoting plant growth and antagonizing pathogens (Fan et al, 2018). Alcaligenes species are another group of potential PGPR (Yokoyama et al, 2013;Ray et al, 2016;Gong et al, 2019;Lahlali et al, 2020), with distinct biotechnological potential in the pharmaceutical industry and bioremediation of contaminated environments (Ray et al, 2015;Dixit et al, 2020;Felestrino et al, 2020;Onajobi et al, 2020;Zouari et al, 2020;Fernandes et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Alcaligenes ammonioxydans HO-1 antagonizes Bacillus velezensis via hydroxylamine-triggered population response

et al. 2022

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Antagonism is a common behavior seen between microbes in nature. Alcaligenes ammonioxydans HO-1 converts ammonia to nitrogen under aerobic conditions, which leads to the accumulation of extracellular hydroxylamine (HA), providing pronounced growth advantages against many bacterial genera, including Bacillus velezensis V4. In contrast, a mutant variant of A. ammonioxydans, strain 2-29, that cannot produce HA fails to antagonize other bacteria. In this article, we demonstrate that cell-free supernatants derived from the antagonistic HO-1 strain were sufficient to reproduce the antagonistic behavior and the efficiency of this inhibition correlated strongly with the HA content of the supernatant. Furthermore, reintroducing the capacity to produce HA to the 2-29 strain or supplementing bacterial co-cultures with HA restored antagonistic behavior. The HA-mediated antagonism was dose-dependent and affected by the temperature, but not by pH. HA caused a decline in biomass, cell aggregation, and hydrolysis of the cell wall in exponentially growing B. velezensis bulk cultures. Analysis of differential gene expression identified a series of genes modulating multicellular behavior in B. velezensis. Genes involved in motility, chemotaxis, sporulation, polypeptide synthesis, and non-ribosomal peptide synthesis were all significantly downregulated in the presence of HA, whereas autolysis-related genes showed upregulation. Taken together, these findings indicate that HA affects the population response of coexisting strains and also suggest that A. ammonioxydans HO-1 antagonize other bacteria by producing extracellular HA that, in turn, acts as a signaling molecule.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alcaligenes ammonioxydans HO-1 antagonizes Bacillus velezensis via hydroxylamine-triggered population response

et al. 2022

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Biologically active metabolites of Alcaligenes faecalis: diversity, statistical optimization, and future perspectives

El-Sayed,

Abdelaziz,

Alshahrani

et al. 2024

Future Science OA

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The The ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIA ASSOCIATED WITH SOIL SURROUNDING THE ROOT OF CITRUS (Citrus sinensis L.) TREE

Olahan,

Ajadi

2024

FJS

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Microbiological processes occur in the soil around the roots of land plants. This dynamic area, where plants and microorganisms interact, is crucial for the health and productivity of these plants. This study was aimed at isolating and molecularly identifying bacteria associated with the soil surrounding the root of an orange tree growing in the University of Ilorin, Nigeria to be able to know their roles, whether beneficial or harmful. Securing the soil is a way of having a food-secured world. Soil samples were collected at four different points from an orange tree trunk into separately labeled Ziploc bags using a separate sterile hand trowel for each collection. The labeled ziploc bags were brought to the University of Ilorin's Biology Laboratory for physicochemical analysis of the soil sample and isolation of bacteria using serial dilution method. Results of the physicochemical assessment of the bulked sample indicated that it is a sandy-loam soil with pH value of 6.7 (slightly neutral). Four bacterial species, i.e. Bacillus cereus, Lysinibacillusmacroides, Leucobacterkomogatae, and Alcaligenesfaecalis were isolated and identified molecularly. According to existing literature, the four bacterial species identified in this study are known for their abilities to enhance plant development and solubilize phosphorus, which are crucial for improving supply of nutrient.

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Whole-Genome Sequencing of Alcaligenes faecalis HZ01, with Potential to Inhibit Nontuberculous Mycobacterial Growth

Cited by 3 publications

References 18 publications

Alcaligenes ammonioxydans HO-1 antagonizes Bacillus velezensis via hydroxylamine-triggered population response

Alcaligenes ammonioxydans HO-1 antagonizes Bacillus velezensis via hydroxylamine-triggered population response

Biologically active metabolites of Alcaligenes faecalis: diversity, statistical optimization, and future perspectives

The The ISOLATION AND MOLECULAR IDENTIFICATION OF BACTERIA ASSOCIATED WITH SOIL SURROUNDING THE ROOT OF CITRUS (Citrus sinensis L.) TREE

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